##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545847_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5620197
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.497063359166948	31.0	31.0	33.0	30.0	34.0
2	31.884360103391394	31.0	31.0	34.0	30.0	34.0
3	31.860939749976737	31.0	31.0	34.0	30.0	34.0
4	35.66272036371679	37.0	35.0	37.0	33.0	37.0
5	35.61364343634218	37.0	35.0	37.0	33.0	37.0
6	35.661421298933114	37.0	35.0	37.0	33.0	37.0
7	35.994985585024864	37.0	35.0	37.0	35.0	37.0
8	35.97992917330122	37.0	35.0	37.0	35.0	37.0
9	37.73954204808123	39.0	38.0	39.0	35.0	39.0
10	37.233463702428935	39.0	37.0	39.0	34.0	39.0
11	36.99654585061698	39.0	37.0	39.0	33.0	39.0
12	36.47929814559881	39.0	35.0	39.0	32.0	39.0
13	36.230035530783	39.0	35.0	39.0	32.0	39.0
14	37.18879249250516	39.0	35.0	41.0	32.0	41.0
15	37.367774652739044	40.0	35.0	41.0	32.0	41.0
16	37.49899087167229	40.0	35.0	41.0	32.0	41.0
17	37.42516872629198	39.0	35.0	41.0	32.0	41.0
18	37.252951987270194	39.0	35.0	41.0	32.0	41.0
19	37.059921742956696	38.0	35.0	41.0	32.0	41.0
20	36.80621497787355	38.0	35.0	41.0	32.0	41.0
21	36.67673357357402	38.0	35.0	41.0	32.0	41.0
22	36.616416292880835	38.0	35.0	40.0	31.0	41.0
23	36.51736762964003	38.0	35.0	40.0	31.0	41.0
24	36.54334750187582	38.0	35.0	40.0	31.0	41.0
25	36.502227590954554	38.0	35.0	40.0	31.0	41.0
26	36.27509267023914	38.0	35.0	40.0	31.0	41.0
27	36.256651679647526	38.0	35.0	40.0	31.0	41.0
28	36.22227103427157	38.0	35.0	40.0	31.0	41.0
29	36.13539756702479	38.0	35.0	40.0	31.0	41.0
30	35.96359593800715	38.0	35.0	40.0	30.0	41.0
31	35.70855114153472	38.0	35.0	40.0	30.0	41.0
32	35.430586685840375	38.0	34.0	40.0	28.0	41.0
33	35.07389118210625	38.0	34.0	40.0	25.0	41.0
34	34.67844596906478	37.0	34.0	40.0	23.0	41.0
35	34.34561368578361	37.0	34.0	40.0	21.0	41.0
36	34.124800429593485	37.0	33.0	40.0	20.0	41.0
37	33.98368615904389	37.0	33.0	40.0	19.0	41.0
38	33.842733626597074	37.0	33.0	40.0	18.0	41.0
39	33.681714181905015	37.0	33.0	40.0	18.0	41.0
40	33.51968979023333	37.0	33.0	40.0	18.0	41.0
41	33.4415478674502	36.0	33.0	40.0	16.0	41.0
42	33.429808065446814	36.0	33.0	40.0	17.0	41.0
43	33.33625956527858	36.0	33.0	40.0	16.0	41.0
44	33.1934821857668	36.0	33.0	40.0	15.0	41.0
45	33.1072170601849	36.0	32.0	40.0	15.0	41.0
46	33.06999452154435	35.0	32.0	40.0	15.0	41.0
47	32.984860139244226	35.0	32.0	40.0	15.0	41.0
48	32.86917576020912	35.0	32.0	40.0	15.0	41.0
49	32.81441130978149	35.0	32.0	40.0	15.0	41.0
50	32.65268993239917	35.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	3.0
12	5.0
13	20.0
14	47.0
15	156.0
16	581.0
17	1396.0
18	3113.0
19	6334.0
20	11180.0
21	17912.0
22	27163.0
23	40291.0
24	59428.0
25	89833.0
26	128955.0
27	157366.0
28	158771.0
29	152046.0
30	159094.0
31	176100.0
32	207875.0
33	263047.0
34	430009.0
35	635973.0
36	388784.0
37	482215.0
38	734660.0
39	1287832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.452580754731553	21.167603199674318	27.680986983196497	19.698829062397635
2	36.890770910699395	23.135149888874	27.757941581051345	12.216137619375264
3	27.321782492677748	23.27578552851439	36.988116252864444	12.414315725943414
4	24.109243857466208	25.807796417100683	36.14311028599175	13.939849439441357
5	22.24375764764118	29.83043121086325	34.29886888306584	13.62694225842973
6	20.22717353146162	37.705546620518824	32.108251721425425	9.959028126594138
7	79.86123973946108	4.008453796192554	14.158880907555377	1.9714255567909809
8	80.40748393695097	3.370255526630116	13.971360790377988	2.250899746040931
9	75.34243016748346	5.891519460972631	15.677920186783489	3.0881301847604274
10	38.588184720215324	30.394948789161663	20.78338179248877	10.233484698134248
11	35.33931283903393	21.361849059739367	29.625634119231055	13.673203981995648
12	31.665313511252364	20.67856340267076	32.33680242881166	15.319320657265218
13	22.26601665386462	28.449732278067835	34.02215260425925	15.262098463808297
14	17.105699319792528	31.021563834862015	36.20899765613198	15.663739189213475
15	16.312292967666437	22.420014814427326	45.48673293836497	15.780959279541268
16	19.76802592506989	20.522554636430005	42.03567953222992	17.67373990627019
17	20.192423859875376	19.73238660495353	34.699317479440666	25.37587205573043
18	21.81272293480104	21.80263432046955	37.634570460786335	18.750072283943073
19	24.927506989523675	23.210076088080186	32.84986273612829	19.012554186267845
20	25.476526890427508	23.87763275913638	32.51136926339059	18.134471087045526
21	22.08860294398933	24.383184432858847	35.334704459647945	18.19350816350388
22	21.219202814420917	22.20352774110943	34.4963174778393	22.08095196663035
23	19.05463456174223	25.39781078848304	33.999075121388096	21.54847952838664
24	19.081466361410463	23.96261198673285	38.76892571559324	18.186995936263443
25	19.069900930518983	24.022503125780112	36.861537059999854	20.04605888370105
26	18.092408504541744	27.402260098711846	33.61597467135049	20.889356725395924
27	18.22042892802512	27.04451463178248	35.221505580676265	19.513550859516133
28	16.629167981122368	25.702355273311596	38.586423216125695	19.082053529440337
29	17.977127848009598	23.93494391744631	37.89966792978965	20.18826030475444
30	20.30346978940418	25.50570736221524	35.66979947500061	18.521023373379972
31	23.863433968595764	24.74818943179394	32.85244271686562	18.53593388274468
32	25.414981005114235	24.325410657313256	32.62419448997962	17.635413847592886
33	24.435638110194358	25.590277351487856	31.174298694511954	18.79978584380583
34	19.81386061734135	25.788829110438655	34.004466391480584	20.392843880739413
35	19.983160732622007	26.72831575120943	32.974502495197235	20.314021020971328
36	25.41515893482026	25.407223269931638	30.114371435734373	19.063246359513734
37	20.7609626495299	29.522844128061703	30.742801364436158	18.97339185797224
38	21.857098603483117	29.217036342320384	28.614744287433343	20.311120766763157
39	21.18363466618697	27.651913269232374	29.388275891396688	21.776176173183963
40	22.801958721375783	26.034781343073917	29.34655137533435	21.81670856021595
41	19.374623345053564	25.151467110494526	29.998076579877893	25.475832964574018
42	21.242867465321947	25.90807759941511	29.75141262841854	23.097642306844403
43	21.19105433492812	25.76925684277615	30.8142579343749	22.225430887920833
44	20.76051782526484	27.713957357722514	30.451815123206533	21.073709693806105
45	18.895102787322223	29.894592662855057	28.632377121300195	22.577927428522525
46	21.61004320667051	27.9483441594663	30.159779096711382	20.28183353715181
47	20.625077733040317	26.87135344188113	31.468843529862035	21.034725295216518
48	20.831921016291776	26.31971797429877	31.29409876557708	21.55426224383238
49	21.398876231562703	24.65845948104666	31.996796553572764	21.945867733817874
50	19.9831073537102	28.029800378883518	30.536474077332166	21.45061819007412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3874.0
1	7746.0
2	11618.0
3	92105.5
4	172593.0
5	117895.0
6	63197.0
7	62285.5
8	61374.0
9	62009.5
10	62645.0
11	61008.5
12	59372.0
13	56614.0
14	53856.0
15	50508.0
16	47160.0
17	44204.5
18	41249.0
19	39711.5
20	38174.0
21	37441.0
22	36708.0
23	39210.5
24	41713.0
25	44838.5
26	47964.0
27	53469.5
28	58975.0
29	68738.0
30	78501.0
31	93104.0
32	107707.0
33	118416.5
34	129126.0
35	145680.5
36	162235.0
37	181448.5
38	200662.0
39	226476.0
40	252290.0
41	296362.5
42	340435.0
43	396795.0
44	453155.0
45	467086.5
46	481018.0
47	461355.5
48	441693.0
49	416528.5
50	391364.0
51	360566.5
52	329769.0
53	304928.5
54	280088.0
55	266886.0
56	253684.0
57	239549.5
58	225415.0
59	207580.5
60	189746.0
61	171620.0
62	153494.0
63	132596.0
64	111698.0
65	96744.0
66	81790.0
67	67817.0
68	53844.0
69	46840.0
70	39836.0
71	32630.5
72	25425.0
73	21146.5
74	16868.0
75	12488.5
76	8109.0
77	6843.0
78	5577.0
79	4135.5
80	2694.0
81	2419.0
82	2144.0
83	1385.5
84	627.0
85	502.5
86	378.0
87	278.5
88	179.0
89	135.5
90	92.0
91	69.5
92	47.0
93	39.5
94	32.0
95	16.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5620197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.961459661352762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.73616187589761	15.119140169609265
2	8.669021836695991	3.287546157197946
3	3.0214261505364157	1.718719502194578
4	1.5028322003359043	1.1398356857780505
5	0.9098373167481522	0.8625921789956761
6	0.6255347570908686	0.7116631238011564
7	0.42283877032794115	0.5612348200780484
8	0.3216400901708194	0.4879012476198286
9	0.26473808004501403	0.45178383830377616
>10	2.4758327119174837	10.395776897106058
>50	0.7602834059964576	10.520601067741246
>100	1.271983402631685	49.906435129525995
>500	0.014620419495553488	1.7712652081064928
>1k	0.0030578655153991603	0.9320722366908383
>5k	0.0	0.0
>10k+	1.9111659471244752E-4	2.1334327372509487
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107167	1.9068192805341166	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	10577	0.18819625006027368	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1000320807259959	0.0
2	0.0	0.0	0.0	0.2537455537590586	0.0
3	0.0	0.0	0.0	0.35770988098815754	0.0
4	1.779297060227604E-5	0.0	0.0	0.5068683535470376	0.0
5	1.779297060227604E-5	0.0	0.0	0.8155763934965269	0.0
6	1.779297060227604E-5	0.0	0.0	1.1317574810989721	0.0
7	1.779297060227604E-5	0.0	0.0	1.3652368413420384	0.0
8	1.779297060227604E-5	0.0	0.0	1.9221390282226762	0.0
9	1.779297060227604E-5	0.0	0.0	2.1973962834398866	0.0
10	1.779297060227604E-5	0.0	0.0	2.6527184011521303	0.0
11	1.779297060227604E-5	0.0	0.0	3.0567433846180125	0.0
12	1.779297060227604E-5	0.0	0.0	3.432637681561696	0.0
13	1.779297060227604E-5	0.0	0.0	3.582490079974065	0.0
14	1.779297060227604E-5	0.0	0.0	3.6435555550810763	0.0
15	1.779297060227604E-5	0.0	0.0	3.735563006065446	0.0
16	1.779297060227604E-5	0.0	0.0	3.9210013456823667	0.0
17	1.779297060227604E-5	0.0	0.0	4.141064806091316	0.0
18	1.779297060227604E-5	0.0	0.0	4.480038689035277	0.0
19	1.779297060227604E-5	0.0	0.0	4.628965852976328	0.0
20	1.779297060227604E-5	0.0	0.0	4.796130811784711	0.0
21	1.779297060227604E-5	0.0	0.0	4.980216885635859	0.0
22	1.779297060227604E-5	0.0	0.0	5.177309620997271	0.0
23	1.779297060227604E-5	0.0	0.0	5.379829212392377	0.0
24	1.779297060227604E-5	0.0	0.0	5.540054912665873	0.0
25	1.779297060227604E-5	0.0	0.0	5.672576957711625	0.0
26	1.779297060227604E-5	0.0	0.0	5.812109433174673	0.0
27	1.779297060227604E-5	0.0	0.0	5.972086031859737	0.0
28	1.779297060227604E-5	0.0	0.0	6.124429446156425	0.0
29	1.779297060227604E-5	0.0	0.0	6.309725442008528	0.0
30	1.779297060227604E-5	0.0	0.0	6.515714662671077	0.0
31	1.779297060227604E-5	0.0	0.0	6.700156595934271	0.0
32	1.779297060227604E-5	0.0	0.0	6.872641652952734	0.0
33	1.779297060227604E-5	0.0	0.0	7.044539541941323	0.0
34	1.779297060227604E-5	0.0	0.0	7.233999092914359	0.0
35	1.779297060227604E-5	0.0	0.0	7.448173080053955	0.0
36	1.779297060227604E-5	0.0	0.0	7.6349103065248425	0.0
37	1.779297060227604E-5	0.0	0.0	7.835081225800448	0.0
38	1.779297060227604E-5	0.0	0.0	8.06727949216015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCGA	30	2.529483E-6	44.0	21
CGTTTTT	57575	0.0	42.914806	1
CGGTCTA	580	0.0	42.862072	31
CGACGGT	590	0.0	42.135593	28
CGTACTA	60	3.6379788E-12	40.333332	21
TACGCGG	390	0.0	40.05128	2
TACGGGA	1725	0.0	38.133335	4
TCGATAG	435	0.0	37.931038	1
GTTTTTT	67410	0.0	37.906837	2
TTCGATC	35	7.295266E-6	37.714287	23
AGGGCGA	4380	0.0	37.42009	6
ACGGGAC	1385	0.0	36.851986	5
TGCGTAG	335	0.0	36.776115	1
CGACAAT	865	0.0	36.369946	20
CGACAGG	835	0.0	36.35928	2
GCGATAT	565	0.0	36.21239	9
ACGGTCT	670	0.0	36.1194	30
CGAATAT	495	0.0	36.0	14
GGGCGAT	8945	0.0	35.760757	7
AACGGGA	2005	0.0	35.660847	4
>>END_MODULE