FastQCFastQC Report
Thu 26 May 2016
SRR1545846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545846_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1959396
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195250.9964805480872677No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36010.1837811243873112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA207.857842E-444.041
GTCGAAT207.857842E-444.030
GCTATCG207.857842E-444.011
GCACGTA302.5286336E-644.026
AAGCGTA207.857842E-444.024
TACGGAC302.5286336E-644.031
CGACACG207.857842E-444.015
GCGCATC207.857842E-444.015
CCCGTAG351.4467878E-744.028
CACTCGA408.3182385E-944.010
GTATCGC254.4439934E-544.015
CGTGTCA502.7284841E-1144.035
CGTCGGT207.857842E-444.042
CGTCCGA207.857842E-444.038
TGTCGAT254.4439934E-544.029
TGCAACG302.5286336E-644.01
CGTTTTT150700.042.9927021
TATTACG900.041.5555571
TGTTACG900.041.5555571
CGTTAGG4100.040.780492
Produced by FastQC (version 0.11.5)