FastQCFastQC Report
Thu 26 May 2016
SRR1545845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545845_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1946288
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226871.1656548260072508No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31260.16061343439408762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATA207.85783E-444.026
TATCGAC207.85783E-444.012
ATCGACG351.4467696E-743.9999961
CGTTTTT157200.043.17431
TCACGAC2150.041.95348725
ACGTAAG1000.041.8000031
ATTAGCG850.041.4117661
TAACGCG700.040.857141
TTGCGAG2500.040.481
ACTACGG452.3523171E-839.111112
ATAGCGG2850.037.8245622
GGTACGA357.2928124E-637.7142837
CTATACG357.2928124E-637.7142831
CCCGTAT357.2928124E-637.71428334
ACCCGTA357.2928124E-637.71428333
TAGGACG700.037.7142831
TACGGGA6400.037.468754
TGTTACG1000.037.41
TCGATTG651.0913936E-1137.2307661
CATACGA950.037.0526318
Produced by FastQC (version 0.11.5)