##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545845_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1946288
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.499499046389847	31.0	31.0	33.0	30.0	34.0
2	31.905054647616385	31.0	31.0	34.0	30.0	34.0
3	31.948249179977473	33.0	31.0	34.0	30.0	34.0
4	35.71028491158554	37.0	35.0	37.0	33.0	37.0
5	35.6561290004357	37.0	35.0	37.0	33.0	37.0
6	35.69705819488174	37.0	35.0	37.0	33.0	37.0
7	35.98110454362355	37.0	35.0	37.0	35.0	37.0
8	35.96854576506663	37.0	35.0	37.0	35.0	37.0
9	37.74948466002976	39.0	38.0	39.0	35.0	39.0
10	37.264547179040306	39.0	37.0	39.0	34.0	39.0
11	37.022282416579664	39.0	37.0	39.0	33.0	39.0
12	36.411054273571025	38.0	35.0	39.0	32.0	39.0
13	36.14514963869684	38.0	35.0	39.0	32.0	39.0
14	37.18151321900972	39.0	35.0	41.0	32.0	41.0
15	37.3368766595694	39.0	35.0	41.0	32.0	41.0
16	37.45204769283888	39.0	35.0	41.0	33.0	41.0
17	37.37674177716762	39.0	35.0	41.0	32.0	41.0
18	37.24908184194734	39.0	35.0	41.0	32.0	41.0
19	37.09352829591509	38.0	35.0	41.0	32.0	41.0
20	36.874865898572054	38.0	35.0	41.0	32.0	41.0
21	36.72378702432528	38.0	35.0	41.0	32.0	41.0
22	36.63645719441316	38.0	35.0	41.0	31.0	41.0
23	36.54274290341409	38.0	35.0	40.0	31.0	41.0
24	36.58447876162212	38.0	35.0	41.0	31.0	41.0
25	36.549885217398455	38.0	35.0	41.0	31.0	41.0
26	36.31675887638417	38.0	35.0	40.0	31.0	41.0
27	36.286150867703036	38.0	35.0	40.0	31.0	41.0
28	36.267394136941704	38.0	35.0	40.0	31.0	41.0
29	36.21092818740084	38.0	35.0	40.0	31.0	41.0
30	36.074716588706295	38.0	35.0	40.0	30.0	41.0
31	35.858335970832684	38.0	35.0	40.0	30.0	41.0
32	35.61074568614717	38.0	35.0	40.0	29.0	41.0
33	35.29237450983616	38.0	34.0	40.0	27.0	41.0
34	34.982949080506074	38.0	34.0	40.0	25.0	41.0
35	34.68024773312069	37.0	34.0	40.0	23.0	41.0
36	34.501509540211934	37.0	34.0	40.0	23.0	41.0
37	34.417363206267524	37.0	34.0	40.0	23.0	41.0
38	34.31704763118305	37.0	33.0	40.0	22.0	41.0
39	34.208600166059696	37.0	33.0	40.0	22.0	41.0
40	34.0566709551721	37.0	33.0	40.0	21.0	41.0
41	33.98985710234046	37.0	33.0	40.0	20.0	41.0
42	33.94853485198491	36.0	33.0	40.0	20.0	41.0
43	33.84478864381839	36.0	33.0	40.0	20.0	41.0
44	33.72736768659109	36.0	33.0	40.0	19.0	41.0
45	33.63287447695305	36.0	33.0	40.0	20.0	41.0
46	33.5806745969764	36.0	33.0	40.0	20.0	41.0
47	33.52944682390273	35.0	33.0	40.0	20.0	41.0
48	33.431062617659876	35.0	33.0	40.0	19.0	41.0
49	33.3784337158735	36.0	33.0	40.0	18.0	41.0
50	33.212108382726505	35.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	3.0
13	12.0
14	16.0
15	77.0
16	165.0
17	492.0
18	1069.0
19	1968.0
20	3471.0
21	5679.0
22	8588.0
23	12423.0
24	18023.0
25	26628.0
26	39135.0
27	48629.0
28	50289.0
29	48854.0
30	51883.0
31	58152.0
32	69018.0
33	88586.0
34	151678.0
35	246807.0
36	129361.0
37	165106.0
38	259245.0
39	460929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.102237695551736	22.107211265753065	27.87372680713235	17.916824231562853
2	35.061820244485915	23.4691371472259	28.810124709190006	12.658917899098181
3	28.03089779107717	23.3648360365989	35.54941509170277	13.054851080621162
4	23.740011755711386	26.711874090576522	35.270576605312264	14.277537548399827
5	22.225847356609094	30.456592241230485	33.333864258527	13.98369614363342
6	20.08084106771454	38.634518632391504	30.842660490122736	10.441979809771215
7	83.14386154567053	3.7830475243129484	11.419532977647705	1.653557952368817
8	84.17546632358624	2.963487418100507	11.050625601144331	1.8104206571689287
9	79.36867513954769	5.32552222487114	12.623465797456493	2.682336838124676
10	41.143653971046426	30.94983887276703	17.31634783752456	10.590159318661986
11	39.04309125884761	20.092041876639016	27.07065963516191	13.794207229351462
12	34.214771914536804	20.617555058655245	29.852365117598218	15.315307909209736
13	21.790608584135544	31.403060595348688	31.400183323331387	15.406147497184383
14	16.926066440321268	32.88706501812682	34.21240844109402	15.974460100457897
15	16.18599097358664	22.03486842646104	46.23123607605863	15.547904523893688
16	19.77317848129362	18.95603322838141	43.153274335555686	18.117513954769286
17	19.29549994656495	19.817416538559556	32.749161480726386	28.13792203414911
18	22.30774684938714	20.940169183594616	36.42133127265852	20.330752694359724
19	26.88153037988211	23.243117154295767	30.599942043520794	19.275410422301327
20	28.111101748559307	22.797705170046775	30.073555403927887	19.01763767746603
21	22.934632490155618	25.34609471979481	32.563937094612925	19.155335695436644
22	23.002608041564248	21.874460511496757	31.29685842999597	23.826073016943024
23	19.992416333040126	26.286140591731545	31.222460396405875	22.498982678822454
24	20.3523836143469	22.596964066982892	38.674594921203855	18.376057397466354
25	19.23533413348898	23.699729947469233	36.45847890959612	20.606457009445673
26	18.83554746265712	29.201125424397624	31.462044671703264	20.501282441241994
27	18.21462188535304	28.425135437304245	33.220109254128886	20.14013342321383
28	17.088169890581455	25.225608954070516	39.145645454321254	18.540575701026775
29	18.70797127660449	22.715651537696374	37.61354948496831	20.962827700730827
30	20.138694787205182	25.680372072375725	34.45091373938492	19.730019401034173
31	26.432573185468954	23.717815657292242	30.24413653066761	19.605474626571194
32	27.727396973109837	23.934484516166158	30.173283707241684	18.16483480348232
33	25.860715372031272	25.02651200644509	29.278863148722078	19.833909472801558
34	20.887658969278956	25.714488297723666	31.220096922963098	22.17775581003428
35	20.926091102652844	25.963372327219815	32.638592027490276	20.47194454263706
36	26.80867374201557	25.304220135971654	28.838383630788456	19.04872249122432
37	21.734810058942973	30.15915424644246	29.29828473483883	18.80775095977574
38	21.96499182032669	30.171896451090486	27.02775745418972	20.8353542743931
39	21.96971876721225	29.415173910541505	28.132475769259223	20.48263155298702
40	24.60920480422219	25.552641746750737	27.62910730580469	22.20904614322238
41	19.88056238336772	24.83666343316097	28.673043249508805	26.609730933962496
42	22.486754272748946	25.309101222429568	26.79382496321202	25.410319541609468
43	22.532379586166076	25.15860961995347	28.0407627237079	24.268248070172554
44	20.95095895365948	28.005516141496017	29.670788701363826	21.372736203480677
45	19.064701626891807	31.69782683754922	27.169514480899025	22.06795705465995
46	21.757571335794086	29.68882303132938	28.167414072326398	20.386191560550134
47	21.336564783834664	26.627302845210988	29.135667486004124	22.90046488495022
48	22.363596754437165	24.9657296350797	31.269781245118917	21.40089236536422
49	21.91895546804995	24.422952820959694	31.92759756007333	21.73049415091703
50	20.152413209144793	30.267462985950694	28.60486217867037	20.975261626234143
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	872.0
1	2090.0
2	3308.0
3	22642.5
4	41977.0
5	29934.0
6	17891.0
7	17819.0
8	17747.0
9	17899.5
10	18052.0
11	17446.5
12	16841.0
13	15886.0
14	14931.0
15	13893.5
16	12856.0
17	12188.0
18	11520.0
19	10426.0
20	9332.0
21	9358.0
22	9384.0
23	10181.0
24	10978.0
25	12425.5
26	13873.0
27	16102.5
28	18332.0
29	21999.5
30	25667.0
31	28979.0
32	32291.0
33	37837.5
34	43384.0
35	48418.0
36	53452.0
37	58623.5
38	63795.0
39	74770.0
40	85745.0
41	109779.5
42	133814.0
43	150998.0
44	168182.0
45	167360.0
46	166538.0
47	159917.0
48	153296.0
49	143354.5
50	133413.0
51	127377.0
52	121341.0
53	115125.0
54	108909.0
55	99858.5
56	90808.0
57	86391.5
58	81975.0
59	76132.0
60	70289.0
61	65887.5
62	61486.0
63	53488.0
64	45490.0
65	38026.0
66	30562.0
67	25487.5
68	20413.0
69	17243.0
70	14073.0
71	12115.0
72	10157.0
73	7949.0
74	5741.0
75	4480.5
76	3220.0
77	2526.0
78	1832.0
79	1501.5
80	1171.0
81	920.5
82	670.0
83	487.0
84	304.0
85	248.0
86	192.0
87	150.5
88	109.0
89	73.0
90	37.0
91	27.5
92	18.0
93	18.5
94	19.0
95	10.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1946288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.204770302041183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.43433741435649	15.447229744395937
2	6.579417512468051	2.527124040963522
3	2.2539412900957427	1.2985927425172534
4	1.2633709782655187	0.9705099777541741
5	0.7442347685432785	0.7146428890333224
6	0.5560564294473251	0.640736160150543
7	0.47544402477472564	0.6391555301093609
8	0.37444116988918386	0.5752845327479484
9	0.31223822752061114	0.5396817095144829
>10	4.224265551326069	19.979121444594377
>50	1.611294943934572	22.244419454166632
>100	1.1687912551709383	32.870770655996054
>500	0.001624825655665449	0.21026567223680687
>1k	2.708042759442415E-4	0.16257494222415492
>5k	0.0	0.0
>10k+	2.708042759442415E-4	1.179890503595458
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22687	1.1656548260072508	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3126	0.16061343439408762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023943013572503145	0.0
2	0.0	0.0	0.0	0.08010119776723691	0.0
3	0.0	0.0	0.0	0.11827643185386746	0.0
4	0.0	0.0	0.0	0.1905165114309907	0.0
5	0.0	0.0	0.0	0.3382849814621474	0.0
6	0.0	0.0	0.0	0.5049098591780867	0.0
7	0.0	0.0	0.0	0.6269370206259299	0.0
8	0.0	0.0	0.0	0.9630126682176533	0.0
9	0.0	0.0	0.0	1.136625206547027	0.0
10	0.0	0.0	0.0	1.410890885624327	0.0
11	0.0	0.0	0.0	1.6027432733490625	0.0
12	0.0	0.0	0.0	1.786940062313491	0.0
13	0.0	0.0	0.0	1.8557890712988006	0.0
14	0.0	0.0	0.0	1.8799889841585624	0.0
15	0.0	0.0	0.0	1.9169824815238032	0.0
16	0.0	0.0	0.0	1.9966212605739746	0.0
17	0.0	0.0	0.0	2.0964523236026733	0.0
18	0.0	0.0	0.0	2.2471494455085783	0.0
19	0.0	0.0	0.0	2.3153305163470153	0.0
20	0.0	0.0	0.0	2.39476377596738	0.0
21	0.0	0.0	0.0	2.4886347755316787	0.0
22	0.0	0.0	0.0	2.5856399464005326	0.0
23	0.0	0.0	0.0	2.6930238484746347	0.0
24	0.0	0.0	0.0	2.7726626275248063	0.0
25	0.0	0.0	0.0	2.8368874493394607	0.0
26	0.0	0.0	0.0	2.9064043964716424	0.0
27	0.0	0.0	0.0	2.9764865220357932	0.0
28	0.0	0.0	0.0	3.0496000591895958	0.0
29	0.0	0.0	0.0	3.1379734140065603	0.0
30	0.0	0.0	0.0	3.2390375936141003	0.0
31	0.0	0.0	0.0	3.3330627327507543	0.0
32	0.0	0.0	0.0	3.4162467219650945	0.0
33	0.0	0.0	0.0	3.50554491421619	0.0
34	0.0	0.0	0.0	3.6036290620915303	0.0
35	0.0	0.0	0.0	3.718514423353584	0.0
36	0.0	0.0	0.0	3.82065757996761	0.0
37	3.0827914471034094E-4	0.0	0.0	3.9353888016573086	0.0
38	3.0827914471034094E-4	0.0	0.0	4.047448270759517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGATA	20	7.85783E-4	44.0	26
TATCGAC	20	7.85783E-4	44.0	12
ATCGACG	35	1.4467696E-7	43.999996	1
CGTTTTT	15720	0.0	43.1743	1
TCACGAC	215	0.0	41.953487	25
ACGTAAG	100	0.0	41.800003	1
ATTAGCG	85	0.0	41.411766	1
TAACGCG	70	0.0	40.85714	1
TTGCGAG	250	0.0	40.48	1
ACTACGG	45	2.3523171E-8	39.11111	2
ATAGCGG	285	0.0	37.824562	2
GGTACGA	35	7.2928124E-6	37.714283	7
CTATACG	35	7.2928124E-6	37.714283	1
CCCGTAT	35	7.2928124E-6	37.714283	34
ACCCGTA	35	7.2928124E-6	37.714283	33
TAGGACG	70	0.0	37.714283	1
TACGGGA	640	0.0	37.46875	4
TGTTACG	100	0.0	37.4	1
TCGATTG	65	1.0913936E-11	37.230766	1
CATACGA	95	0.0	37.05263	18
>>END_MODULE