FastQCFastQC Report
Thu 26 May 2016
SRR1545844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545844_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1401997
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152401.0870208709433757No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14920.10641962857267169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAA207.857074E-444.00000425
ATACTCG254.4433426E-544.044
CGTTTTT95950.042.5554961
TACGGGA4850.041.278354
ACGGGTA1050.039.809525
TACGGGT950.039.3684234
CGGTCTA1850.039.2432431
TTACGGG4500.038.6222233
CGGGTAC1100.038.0000046
AGGGATC14050.037.8932386
TTAGCGG3550.037.802822
TATTGCG1650.037.3333321
AACGGGA5250.037.2952354
CGACGGT1950.037.2307728
ATAGGGC3850.037.1428534
AAGGGAT18850.037.114065
GACCGAT6000.037.0333339
AGTACGG1850.036.8648642
CGCATGG900.036.6666682
GCGAATC301.3012414E-436.66666418
Produced by FastQC (version 0.11.5)