##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545844_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1401997
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486047402383885	31.0	31.0	33.0	30.0	34.0
2	31.89619663950779	31.0	31.0	34.0	30.0	34.0
3	31.954209602445655	31.0	31.0	34.0	30.0	34.0
4	35.719247616079066	37.0	35.0	37.0	33.0	37.0
5	35.651343048522925	37.0	35.0	37.0	33.0	37.0
6	35.69444941750945	37.0	35.0	37.0	33.0	37.0
7	35.9780056590706	37.0	35.0	37.0	35.0	37.0
8	35.95929235226609	37.0	35.0	37.0	35.0	37.0
9	37.746311867999715	39.0	38.0	39.0	35.0	39.0
10	37.246653880143825	39.0	37.0	39.0	34.0	39.0
11	36.99584449895399	39.0	37.0	39.0	33.0	39.0
12	36.41431543719423	38.0	35.0	39.0	32.0	39.0
13	36.14941401443798	38.0	35.0	39.0	32.0	39.0
14	37.122413956663245	39.0	35.0	41.0	32.0	41.0
15	37.295113327631945	39.0	35.0	41.0	32.0	41.0
16	37.44052804677899	39.0	35.0	41.0	32.0	41.0
17	37.367313196818536	39.0	35.0	41.0	32.0	41.0
18	37.244119637916484	39.0	35.0	41.0	32.0	41.0
19	37.11454304110494	38.0	35.0	41.0	32.0	41.0
20	36.91567599645363	38.0	35.0	41.0	32.0	41.0
21	36.78250452747046	38.0	35.0	41.0	32.0	41.0
22	36.71125330510693	38.0	35.0	41.0	31.0	41.0
23	36.62653343766071	38.0	35.0	40.0	31.0	41.0
24	36.66944365786803	38.0	35.0	41.0	31.0	41.0
25	36.630522747195606	38.0	35.0	41.0	31.0	41.0
26	36.394467320543484	38.0	35.0	40.0	31.0	41.0
27	36.372340311712506	38.0	35.0	40.0	31.0	41.0
28	36.3454044480837	38.0	35.0	40.0	31.0	41.0
29	36.271317271007	38.0	35.0	40.0	31.0	41.0
30	36.146369072116414	38.0	35.0	40.0	30.0	41.0
31	35.9507473981756	38.0	35.0	40.0	30.0	41.0
32	35.747274066920255	38.0	35.0	40.0	29.0	41.0
33	35.48444326200413	38.0	34.0	40.0	28.0	41.0
34	35.18716231204489	38.0	34.0	40.0	26.0	41.0
35	34.91862821389775	38.0	34.0	40.0	24.0	41.0
36	34.72441167848433	37.0	34.0	40.0	23.0	41.0
37	34.62874742242673	37.0	34.0	40.0	23.0	41.0
38	34.52138841951873	37.0	34.0	40.0	23.0	41.0
39	34.39637032033592	37.0	34.0	40.0	23.0	41.0
40	34.258118954605465	37.0	33.0	40.0	22.0	41.0
41	34.19954108318349	37.0	33.0	40.0	22.0	41.0
42	34.16199678030695	37.0	33.0	40.0	22.0	41.0
43	34.04625473520985	36.0	33.0	40.0	21.0	41.0
44	33.922636781676424	36.0	33.0	40.0	21.0	41.0
45	33.826528872743665	36.0	33.0	40.0	21.0	41.0
46	33.78632336588452	36.0	33.0	40.0	21.0	41.0
47	33.71273262353628	36.0	33.0	40.0	21.0	41.0
48	33.58152763522318	35.0	33.0	40.0	20.0	41.0
49	33.51400181312799	36.0	33.0	40.0	20.0	41.0
50	33.35399719114948	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	8.0
14	23.0
15	44.0
16	131.0
17	306.0
18	679.0
19	1411.0
20	2422.0
21	3908.0
22	5913.0
23	8412.0
24	12116.0
25	17908.0
26	26207.0
27	32171.0
28	34185.0
29	34109.0
30	37239.0
31	42291.0
32	50894.0
33	65262.0
34	110609.0
35	168227.0
36	98632.0
37	126514.0
38	192812.0
39	329559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.222320732497998	22.38178826345563	28.96767967406492	17.428211329981448
2	34.4027840287818	23.191775731331806	29.465968900076106	12.939471339810286
3	27.669674043525056	23.16559878516145	35.96755199904137	13.197175172272125
4	25.303620478503163	27.019815306309503	34.04507998233947	13.63148423284786
5	22.997267469188593	30.907412783336913	32.047857449053026	14.047462298421465
6	20.071583605385747	40.09887325008542	29.14457020949403	10.684972935034812
7	84.86466090868954	3.5411630695358123	9.885898471965348	1.708277549809308
8	85.75531901994084	2.7204052505105216	9.715356024299624	1.808919705249013
9	81.16764871822122	5.049083557240137	11.251949897182376	2.531317827356264
10	41.52540982612659	31.93102410347526	16.33869402003	10.204872050368154
11	38.691951551964806	21.324368026465105	25.849056738352505	14.134623683217582
12	34.61854768590803	21.10104372548586	28.415538692308186	15.864869896297924
13	24.105686388772586	29.544642392244775	29.659335932958484	16.69033528602415
14	17.766942439962424	32.0157603760921	32.658843064571464	17.558454119374005
15	17.073431683520006	22.825726445919642	43.218637415058666	16.882204455501686
16	20.84398183448324	20.406534393440214	39.87447904667414	18.87500472540241
17	21.67715052172009	19.899614621143982	31.406771911780123	27.0164629453558
18	23.241989818808456	21.85040338888029	34.66355491488213	20.244051877429126
19	26.98622036994373	22.80275920704538	29.573886392053623	20.63713403095727
20	28.18971795232087	23.419236988381574	28.799419684920867	19.591625374376694
21	23.609109006652655	24.80875494027448	31.81682985056316	19.765306202509706
22	23.250976999237515	21.928149632274536	31.25862608835825	23.5622472801297
23	20.85396759051553	25.902266552638842	30.652633350855957	22.59113250598967
24	20.97172818486773	23.37066341796737	36.26819458244204	19.389413814722857
25	20.06673338102721	24.150978925061896	34.318190409822556	21.46409728408834
26	19.34105422479506	28.083512304234603	30.54528647350886	22.03014699746148
27	19.472866204421265	27.909403515128776	32.090296912190254	20.527433368259704
28	18.535132386160598	25.289069805427545	36.55314526350627	19.622652544905588
29	19.631211764361836	23.024015029989364	36.086382495825596	21.258390709823203
30	22.188635211059655	25.162464684303888	33.10192532508985	19.546974779546602
31	26.056831790652907	24.179081695609906	29.725669883744406	20.03841662999279
32	28.05847658732508	23.91417385343906	29.324884432705634	18.702465126530228
33	27.26853195834228	25.061537221548974	27.705194804268483	19.964736015840263
34	21.72151580923497	25.665461481016006	30.89985213948389	21.713170570265127
35	21.568163127310545	25.68143869066767	31.345359512181552	21.405038669840234
36	26.881155951118295	25.060324665459344	28.587436349721145	19.471083033701213
37	21.80717933062624	29.637723903831464	29.09264427812613	19.462452487416165
38	22.778936046225493	28.13501027462969	27.178374846736475	21.907678832408344
39	21.656394414538692	28.103412489470376	27.95105838314918	22.289134712841754
40	23.842989678294604	25.270310849452603	28.167250001248217	22.719449471004573
41	21.088276223130293	24.23885357814603	28.724526514678704	25.94834368404497
42	22.9246567574681	24.88528862757909	27.405336815984626	24.784717798968188
43	22.27650986414379	25.694491500338444	28.361544282905026	23.667454352612737
44	21.74648019931569	27.239002651218225	29.343928695995785	21.670588453470298
45	20.223367097076526	30.338296016325284	26.807190029650563	22.631146856947627
46	22.09163072388885	28.283298751709168	28.081943114000957	21.54312741040102
47	21.89056039349585	26.58807401157064	29.146068072898874	22.37529752203464
48	22.80832269969194	24.481079488757825	31.038154860531087	21.672442951019153
49	22.333500000356636	24.39891098197785	31.27175022485783	21.99583879280769
50	20.939417131420395	28.85469797724246	28.791502406923836	21.414382484413306
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	478.0
1	1464.0
2	2450.0
3	14729.5
4	27009.0
5	19118.0
6	11227.0
7	11077.0
8	10927.0
9	10843.0
10	10759.0
11	10594.5
12	10430.0
13	9650.0
14	8870.0
15	8337.0
16	7804.0
17	7405.5
18	7007.0
19	6786.5
20	6566.0
21	6708.5
22	6851.0
23	7418.0
24	7985.0
25	8555.0
26	9125.0
27	10807.5
28	12490.0
29	13954.5
30	15419.0
31	20019.0
32	24619.0
33	27288.0
34	29957.0
35	34728.0
36	39499.0
37	43266.0
38	47033.0
39	53920.5
40	60808.0
41	72808.0
42	84808.0
43	99889.0
44	114970.0
45	116524.5
46	118079.0
47	111778.5
48	105478.0
49	101818.0
50	98158.0
51	93332.5
52	88507.0
53	83467.5
54	78428.0
55	73512.5
56	68597.0
57	66173.5
58	63750.0
59	61056.0
60	58362.0
61	53207.5
62	48053.0
63	43280.5
64	38508.0
65	32329.5
66	26151.0
67	22153.0
68	18155.0
69	15303.0
70	12451.0
71	10598.0
72	8745.0
73	7051.0
74	5357.0
75	4604.0
76	3851.0
77	2898.0
78	1945.0
79	1587.5
80	1230.0
81	965.5
82	701.0
83	459.0
84	217.0
85	147.5
86	78.0
87	59.5
88	41.0
89	39.5
90	38.0
91	26.5
92	15.0
93	10.5
94	6.0
95	5.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1401997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.39025574276441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.09511427515592	16.704744666059966
2	6.659686369495045	2.849047892202025
3	2.461881622164706	1.5798083251954431
4	1.4192243566198306	1.2143028777783382
5	0.9759992814344403	1.0438437117318484
6	0.7204230686068916	0.9246020208293113
7	0.6207044479877956	0.9293918818191249
8	0.4991563107331639	0.8541664913757725
9	0.4222340871674338	0.8128525597041681
>10	5.78155713576141	29.79662469980111
>50	1.8038447838966944	27.270998274501718
>100	0.5394990431504382	14.811308360141764
>500	0.0	0.0
>1k	3.3760891311028284E-4	0.10774539160758789
>5k	0.0	0.0
>10k+	3.3760891311028284E-4	1.1005628472517692
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15240	1.0870208709433757	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1492	0.10641962857267169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.132682880205878E-5	0.0	0.0	0.029457980295250274	0.0
2	7.132682880205878E-5	0.0	0.0	0.10948668221116023	0.0
3	7.132682880205878E-5	0.0	0.0	0.15321002826682226	0.0
4	7.132682880205878E-5	0.0	0.0	0.22503614487049545	0.0
5	7.132682880205878E-5	0.0	0.0	0.37632034875966214	0.0
6	7.132682880205878E-5	0.0	0.0	0.5699726889572517	0.0
7	7.132682880205878E-5	0.0	0.0	0.7040671271051222	0.0
8	7.132682880205878E-5	0.0	0.0	1.1243248024068526	0.0
9	7.132682880205878E-5	0.0	0.0	1.3462938936388593	0.0
10	7.132682880205878E-5	0.0	0.0	1.6911591108968136	0.0
11	7.132682880205878E-5	0.0	0.0	1.9412309726768318	0.0
12	7.132682880205878E-5	0.0	0.0	2.1642699663408695	0.0
13	7.132682880205878E-5	0.0	0.0	2.2518593121097976	0.0
14	7.132682880205878E-5	0.0	0.0	2.2878080338260354	0.0
15	7.132682880205878E-5	0.0	0.0	2.3349550676641964	0.0
16	7.132682880205878E-5	0.0	0.0	2.4315315938621835	0.0
17	7.132682880205878E-5	0.0	0.0	2.543871349225426	0.0
18	7.132682880205878E-5	0.0	0.0	2.712131338369483	0.0
19	7.132682880205878E-5	0.0	0.0	2.7905908500517476	0.0
20	7.132682880205878E-5	0.0	0.0	2.886739415276923	0.0
21	7.132682880205878E-5	0.0	0.0	2.997295999920114	0.0
22	7.132682880205878E-5	0.0	0.0	3.1119895406338243	0.0
23	7.132682880205878E-5	0.0	0.0	3.230606056931648	0.0
24	7.132682880205878E-5	0.0	0.0	3.323830222175939	0.0
25	7.132682880205878E-5	0.0	0.0	3.4057847484695047	0.0
26	7.132682880205878E-5	0.0	0.0	3.4892371381679133	0.0
27	7.132682880205878E-5	0.0	0.0	3.5898792936076185	0.0
28	7.132682880205878E-5	0.0	0.0	3.6873117417512304	0.0
29	7.132682880205878E-5	0.0	0.0	3.7975116922504113	0.0
30	7.132682880205878E-5	0.0	0.0	3.9215490475371917	0.0
31	7.132682880205878E-5	0.0	0.0	4.026827446849031	0.0
32	7.132682880205878E-5	0.0	0.0	4.13039400226962	0.0
33	7.132682880205878E-5	0.0	0.0	4.238382821075937	0.0
34	7.132682880205878E-5	0.0	0.0	4.353575649591262	0.0
35	7.132682880205878E-5	0.0	0.0	4.484460380443039	0.0
36	7.132682880205878E-5	0.0	0.0	4.601008418705604	0.0
37	7.132682880205878E-5	0.0	0.0	4.728326808117278	0.0
38	7.132682880205878E-5	0.0	0.0	4.884675216851392	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTAA	20	7.857074E-4	44.000004	25
ATACTCG	25	4.4433426E-5	44.0	44
CGTTTTT	9595	0.0	42.555496	1
TACGGGA	485	0.0	41.27835	4
ACGGGTA	105	0.0	39.80952	5
TACGGGT	95	0.0	39.368423	4
CGGTCTA	185	0.0	39.24324	31
TTACGGG	450	0.0	38.622223	3
CGGGTAC	110	0.0	38.000004	6
AGGGATC	1405	0.0	37.893238	6
TTAGCGG	355	0.0	37.80282	2
TATTGCG	165	0.0	37.333332	1
AACGGGA	525	0.0	37.295235	4
CGACGGT	195	0.0	37.23077	28
ATAGGGC	385	0.0	37.142853	4
AAGGGAT	1885	0.0	37.11406	5
GACCGAT	600	0.0	37.033333	9
AGTACGG	185	0.0	36.864864	2
CGCATGG	90	0.0	36.666668	2
GCGAATC	30	1.3012414E-4	36.666664	18
>>END_MODULE