FastQCFastQC Report
Thu 26 May 2016
SRR1545843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545843_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2363278
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263861.1165000478149416No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31740.13430497808552358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG302.5288573E-644.034
CGTTTTT170650.042.633461
CGTAAGG3350.040.0597042
TACGGGA10600.040.0566064
CGGGATA6400.039.18756
CGTTCGA750.038.1333314
GCGGGAT15650.037.8146975
ATAGGGC10550.037.7440764
ACGACTA357.2934727E-637.71428721
GGGCGAT33500.037.629857
ACGGGAC6200.037.6129045
CGGTCTA2750.037.631
TAAGGGA32000.037.468754
TATTACG651.0913936E-1137.230771
AGGGATC22900.037.179046
GTTTTTT205800.036.9980552
GACCGAT8100.036.938279
CGACGGT2800.036.92857428
GCGTAAG1550.036.9032251
CGCTAAT301.3015115E-436.66666418
Produced by FastQC (version 0.11.5)