##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545843_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2363278
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519309619943147	31.0	31.0	33.0	30.0	34.0
2	31.925952850235987	31.0	31.0	34.0	30.0	34.0
3	32.00694839963813	33.0	31.0	34.0	30.0	34.0
4	35.7838472663817	37.0	35.0	37.0	35.0	37.0
5	35.72709304618416	37.0	35.0	37.0	33.0	37.0
6	35.76595347648478	37.0	35.0	37.0	33.0	37.0
7	35.972605000342746	37.0	35.0	37.0	35.0	37.0
8	35.94309810356632	37.0	35.0	37.0	35.0	37.0
9	37.73402917473103	39.0	38.0	39.0	35.0	39.0
10	37.29763362583665	39.0	37.0	39.0	34.0	39.0
11	37.08577196588806	39.0	37.0	39.0	33.0	39.0
12	36.624656515230114	39.0	35.0	39.0	33.0	39.0
13	36.41932096012403	39.0	35.0	39.0	32.0	39.0
14	37.49281125622969	40.0	35.0	41.0	32.0	41.0
15	37.588518151482816	40.0	35.0	41.0	33.0	41.0
16	37.68689125866699	40.0	35.0	41.0	33.0	41.0
17	37.61147947892715	40.0	35.0	41.0	33.0	41.0
18	37.47291897102245	39.0	36.0	41.0	33.0	41.0
19	37.34092053495188	39.0	36.0	41.0	32.0	41.0
20	37.15945182919656	39.0	35.0	41.0	32.0	41.0
21	37.05001654481614	39.0	35.0	41.0	32.0	41.0
22	36.963400412477924	38.0	35.0	41.0	32.0	41.0
23	36.86112594455667	38.0	35.0	41.0	32.0	41.0
24	36.91310290198614	38.0	35.0	41.0	32.0	41.0
25	36.87390226625899	38.0	35.0	41.0	32.0	41.0
26	36.63775611671585	38.0	35.0	41.0	31.0	41.0
27	36.62610450399826	38.0	35.0	41.0	31.0	41.0
28	36.5723512003243	38.0	35.0	41.0	31.0	41.0
29	36.49239488540916	38.0	35.0	41.0	31.0	41.0
30	36.36785558025759	38.0	35.0	41.0	31.0	41.0
31	36.18920710978565	38.0	35.0	41.0	30.0	41.0
32	35.97478417689328	38.0	35.0	41.0	30.0	41.0
33	35.69588173714646	38.0	35.0	41.0	28.0	41.0
34	35.40242493688851	38.0	35.0	41.0	26.0	41.0
35	35.143484600626756	38.0	34.0	40.0	24.0	41.0
36	34.978174383208405	38.0	34.0	40.0	24.0	41.0
37	34.88985595431431	38.0	34.0	40.0	23.0	41.0
38	34.77027459317101	38.0	34.0	40.0	23.0	41.0
39	34.651582251432124	38.0	34.0	40.0	23.0	41.0
40	34.519897362900174	38.0	34.0	40.0	23.0	41.0
41	34.45579022019415	38.0	34.0	40.0	22.0	41.0
42	34.42173921138351	37.0	34.0	40.0	22.0	41.0
43	34.329397133980855	37.0	33.0	40.0	22.0	41.0
44	34.18622861973919	37.0	33.0	40.0	21.0	41.0
45	34.082110949283155	37.0	33.0	40.0	22.0	41.0
46	34.037970987755145	37.0	33.0	40.0	22.0	41.0
47	33.97329429715844	36.0	33.0	40.0	21.0	41.0
48	33.85153418260568	36.0	33.0	40.0	20.0	41.0
49	33.779846044350265	36.0	33.0	40.0	20.0	41.0
50	33.633776898020464	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	5.0
13	9.0
14	24.0
15	83.0
16	207.0
17	479.0
18	1072.0
19	2113.0
20	3787.0
21	5792.0
22	9153.0
23	13248.0
24	19516.0
25	29023.0
26	42364.0
27	53059.0
28	55409.0
29	55660.0
30	59216.0
31	67751.0
32	80297.0
33	102997.0
34	171610.0
35	255618.0
36	165100.0
37	218015.0
38	337969.0
39	613698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.92126444709425	22.092915010421965	27.363517961069327	17.62230258141446
2	33.958213972287645	23.933197871769636	28.878109134854213	13.230479021088504
3	28.30043693547691	23.990956628885808	34.47469997181881	13.233906463818476
4	25.253863489610616	26.600467655519154	33.49635548589713	14.649313368973097
5	23.22655227188676	31.07260339240665	32.01675807924417	13.684086256462422
6	20.371069336743286	39.908550750271445	28.817980787702506	10.902399125282763
7	84.34314541073881	3.8059001099320517	10.247249794565008	1.6037046847641285
8	85.12891839216546	3.078816795992685	10.035383056923477	1.7568817549183804
9	79.91230824304209	5.939419738177227	11.413765117772856	2.7345069010078373
10	38.164320913578514	34.91002751263288	16.54223498039587	10.383416593392738
11	36.08191672752846	21.788422690855665	26.747763064692347	15.381897516923527
12	32.72213425589372	21.322925191196298	29.601299550878057	16.353641002031925
13	23.399320773941955	28.243947601594055	31.1671331091814	17.189598515282587
14	17.87216738784011	30.431206146716548	33.977213006679705	17.719413458763633
15	18.249863113861338	23.15444903223404	40.989422319337805	17.606265534566816
16	21.58192984490187	21.492223936413744	38.321687080402725	18.60415913828166
17	21.562803868186474	21.08871660464829	31.810984573122585	25.53749495404265
18	23.5860529315637	22.573941787635647	33.969638781387545	19.8703664994131
19	26.22052928178572	24.04224132751204	29.450280500220455	20.286948890481778
20	27.089237914456106	23.995145725555776	29.524457131154268	19.39115922883385
21	24.269129573414556	24.714485557771877	31.351537990875382	19.664846877938185
22	23.401944248624158	22.88351180013524	30.567330631436505	23.147213319804102
23	21.137293200376764	25.677131509708122	30.99169881833623	22.193876471578882
24	21.47030522858504	23.396528042828646	35.64383876970885	19.48932795887746
25	20.87651135414454	24.65918101890679	33.00056108506913	21.46374654187954
26	20.37187330479106	27.86371302910618	30.374251357648145	21.390162308454613
27	20.35951758532005	26.552821970161784	32.32802065605485	20.759639788463314
28	18.862867593232792	25.740771927805362	35.03946636832399	20.356894110637853
29	20.211799035069085	23.875735313407905	34.41330220143377	21.499163450089238
30	21.77924052946797	25.402174437370466	32.543314836426354	20.275270196735214
31	25.77178816880621	25.064127030336675	29.27945844712302	19.88462635373409
32	26.233350456442277	24.902360196303608	29.723629636462572	19.140659710791535
33	24.983518655020696	26.040525067300592	29.147142232103036	19.82881404557568
34	21.185108142165245	26.682599338715125	31.261535883632817	20.870756635486813
35	21.345436296533883	26.626702402341156	31.320479435766764	20.7073818653582
36	25.50715573876624	27.230524720324905	27.752003784573798	19.510315756335057
37	22.099981466420793	30.09070452143167	27.989216672774003	19.820097339373532
38	22.09625782493638	29.836481361904948	26.615023708594588	21.452237104564084
39	22.969028611953394	28.204680109576614	27.535482495076753	21.290808783393235
40	23.95427029744279	26.1045886264756	27.703257932414218	22.237883143667396
41	20.58682897230034	25.878842861483076	28.33598078600994	25.19834738020664
42	22.200477472392162	26.71992038177481	26.928359676686362	24.15124246914667
43	22.473911236849833	25.594280486679942	28.38447275352286	23.547335522947364
44	22.20919417859431	27.372869378888137	28.453614005631163	21.964322436886395
45	20.509732667929885	29.888696970902277	26.882998953148974	22.71857140801886
46	21.920738905875652	28.528721546936076	28.35413353824645	21.196406008941818
47	21.49852873847258	26.790246428900875	29.337936544071415	22.373288288555134
48	22.25683986395168	25.493826794816353	30.2583530164458	21.990980324786165
49	22.11834578919619	25.546761743645902	30.478682575642814	21.856209891515093
50	20.515233501940948	28.541246522838193	29.26050172683874	21.68301824838212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	899.0
1	2509.5
2	4120.0
3	27324.5
4	50529.0
5	35556.0
6	20583.0
7	19659.5
8	18736.0
9	18418.0
10	18100.0
11	17332.0
12	16564.0
13	15703.0
14	14842.0
15	13830.0
16	12818.0
17	12069.0
18	11320.0
19	11119.0
20	10918.0
21	11155.0
22	11392.0
23	12791.5
24	14191.0
25	15900.0
26	17609.0
27	21488.0
28	25367.0
29	30933.5
30	36500.0
31	41999.0
32	47498.0
33	51885.0
34	56272.0
35	64723.5
36	73175.0
37	77354.5
38	81534.0
39	92584.0
40	103634.0
41	120846.5
42	138059.0
43	154796.5
44	171534.0
45	177142.5
46	182751.0
47	177077.0
48	171403.0
49	162652.5
50	153902.0
51	151541.5
52	149181.0
53	141449.5
54	133718.0
55	127465.0
56	121212.0
57	116954.5
58	112697.0
59	108878.0
60	105059.0
61	93695.5
62	82332.0
63	72476.5
64	62621.0
65	53309.0
66	43997.0
67	36627.5
68	29258.0
69	25641.0
70	22024.0
71	18165.0
72	14306.0
73	11893.0
74	9480.0
75	7672.5
76	5865.0
77	4700.5
78	3536.0
79	2714.0
80	1892.0
81	1440.5
82	989.0
83	654.0
84	319.0
85	321.5
86	324.0
87	209.0
88	94.0
89	81.5
90	69.0
91	50.0
92	31.0
93	23.0
94	15.0
95	10.5
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2363278.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.947342893926535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53780877784459	14.880827930514547
2	6.51632144344519	2.4693395359200463
3	2.3327097668932364	1.3259595547601275
4	1.3465421926405952	1.0205358658040415
5	0.9441611510758454	0.8944672538279208
6	0.6699506330734071	0.7616270620107002
7	0.5431789630090184	0.7204258645429517
8	0.4283993925321929	0.6493624148685833
9	0.397232228782228	0.6773845722529992
>10	5.502003681514246	25.6043282658747
>50	1.8505802551825281	24.874893811078234
>100	0.9277141238734696	24.418049618222387
>500	0.002264926755580211	0.2659831032914269
>1k	9.059707022320843E-4	0.3044772697406702
>5k	0.0	0.0
>10k+	2.2649267555802108E-4	1.1323378772906727
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26386	1.1165000478149416	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3174	0.13430497808552358	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02378052857090871	0.0
2	0.0	0.0	0.0	0.06947976497051976	0.0
3	0.0	0.0	0.0	0.1048120449646635	0.0
4	0.0	0.0	0.0	0.15025739671761004	0.0
5	0.0	0.0	0.0	0.2557041532989348	0.0
6	0.0	0.0	0.0	0.3980911259699451	0.0
7	0.0	0.0	0.0	0.5041725941679311	0.0
8	0.0	0.0	0.0	0.7659276648790366	0.0
9	0.0	0.0	0.0	0.9138577856688888	0.0
10	0.0	0.0	0.0	1.135879909176999	0.0
11	0.0	0.0	0.0	1.3019204680955858	0.0
12	0.0	0.0	0.0	1.4663530909186309	0.0
13	0.0	0.0	0.0	1.5339710351469442	0.0
14	4.2314107777417635E-5	0.0	0.0	1.5635909105911365	0.0
15	4.2314107777417635E-5	0.0	0.0	1.6019698063452543	0.0
16	4.2314107777417635E-5	0.0	0.0	1.674284616536861	0.0
17	4.2314107777417635E-5	0.0	0.0	1.758574319229477	0.0
18	4.2314107777417635E-5	0.0	0.0	1.887843918489488	0.0
19	4.2314107777417635E-5	0.0	0.0	1.9508073108622854	0.0
20	4.2314107777417635E-5	0.0	0.0	2.0195254218928116	0.0
21	4.2314107777417635E-5	0.0	0.0	2.0999645407776826	0.0
22	4.2314107777417635E-5	0.0	0.0	2.1937749177202175	0.0
23	4.2314107777417635E-5	0.0	0.0	2.288177692171636	0.0
24	4.2314107777417635E-5	0.0	0.0	2.366289535128749	0.0
25	4.2314107777417635E-5	0.0	0.0	2.4324264855848527	0.0
26	4.2314107777417635E-5	0.0	0.0	2.5003829426753854	0.0
27	4.2314107777417635E-5	0.0	0.0	2.575405855764747	0.0
28	4.2314107777417635E-5	0.0	0.0	2.6529676153207538	0.0
29	4.2314107777417635E-5	0.0	0.0	2.7418272416533305	0.0
30	4.2314107777417635E-5	0.0	0.0	2.8511245820424005	0.0
31	4.2314107777417635E-5	0.0	0.0	2.9438347921827224	0.0
32	4.2314107777417635E-5	0.0	0.0	3.039506989867464	0.0
33	4.2314107777417635E-5	0.0	0.0	3.134459847719989	0.0
34	4.2314107777417635E-5	0.0	0.0	3.237917841235775	0.0
35	4.2314107777417635E-5	0.0	0.0	3.358343791970306	0.0
36	4.2314107777417635E-5	0.0	0.0	3.4648484012460656	0.0
37	4.2314107777417635E-5	0.0	0.0	3.578546408843987	0.0
38	4.2314107777417635E-5	0.0	0.0	3.7227528881494263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	30	2.5288573E-6	44.0	34
CGTTTTT	17065	0.0	42.63346	1
CGTAAGG	335	0.0	40.059704	2
TACGGGA	1060	0.0	40.056606	4
CGGGATA	640	0.0	39.1875	6
CGTTCGA	75	0.0	38.13333	14
GCGGGAT	1565	0.0	37.814697	5
ATAGGGC	1055	0.0	37.744076	4
ACGACTA	35	7.2934727E-6	37.714287	21
GGGCGAT	3350	0.0	37.62985	7
ACGGGAC	620	0.0	37.612904	5
CGGTCTA	275	0.0	37.6	31
TAAGGGA	3200	0.0	37.46875	4
TATTACG	65	1.0913936E-11	37.23077	1
AGGGATC	2290	0.0	37.17904	6
GTTTTTT	20580	0.0	36.998055	2
GACCGAT	810	0.0	36.93827	9
CGACGGT	280	0.0	36.928574	28
GCGTAAG	155	0.0	36.903225	1
CGCTAAT	30	1.3015115E-4	36.666664	18
>>END_MODULE