FastQCFastQC Report
Thu 26 May 2016
SRR1545842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545842_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2185736
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA153730.703332881921696No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83760.3832118792022458No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC50690.23191272871014612No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT30230.1383058155239242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG408.3182385E-944.01
ACGCGAA254.4441622E-544.030
TATCATC551.8189894E-1244.041
TATTTCG351.4468787E-744.01
TCTTGTC302.5287682E-644.034
AACCGAG800.044.01
ATCTCGA254.4441622E-544.012
ACCGGTT207.858041E-444.034
AGTCCAT254.4441622E-544.027
GTACCAT502.7284841E-1144.038
AAATCCG351.4468787E-744.021
CGTATTG207.858041E-444.038
GCGACGG1650.044.02
ACGCCAT302.5287682E-644.034
GCGACAG351.4468787E-744.01
TACTTGC950.044.019
ATCTACG302.5287682E-644.01
GTCGACG254.4441622E-544.01
GTCTGGC254.4441622E-544.021
AGTCAAT950.044.014
Produced by FastQC (version 0.11.5)