##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545842_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2185736
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6835034057178	31.0	31.0	33.0	30.0	34.0
2	32.109077674522446	33.0	31.0	34.0	30.0	34.0
3	32.152498746417685	33.0	31.0	34.0	30.0	34.0
4	35.82833928708682	37.0	35.0	37.0	35.0	37.0
5	35.78726570820996	37.0	35.0	37.0	35.0	37.0
6	35.849243916008156	37.0	35.0	37.0	35.0	37.0
7	36.08440269090137	37.0	35.0	37.0	35.0	37.0
8	36.046959010603295	37.0	35.0	37.0	35.0	37.0
9	37.870353052701695	39.0	39.0	39.0	35.0	39.0
10	37.59434625224638	39.0	37.0	39.0	35.0	39.0
11	37.26965150411578	39.0	37.0	39.0	34.0	39.0
12	36.06347701643748	37.0	35.0	39.0	33.0	39.0
13	35.638547839263296	37.0	35.0	39.0	32.0	39.0
14	36.49508266323106	38.0	35.0	41.0	32.0	41.0
15	36.84159203124256	38.0	35.0	41.0	33.0	41.0
16	36.99181282643467	38.0	35.0	41.0	33.0	41.0
17	36.90147346248586	37.0	35.0	41.0	33.0	41.0
18	36.87608521797692	37.0	35.0	41.0	33.0	41.0
19	36.825213566505745	37.0	35.0	41.0	33.0	41.0
20	36.64626103060937	36.0	35.0	41.0	33.0	41.0
21	36.47828649022572	36.0	35.0	41.0	32.0	41.0
22	36.444661660877614	36.0	35.0	41.0	32.0	41.0
23	36.45500646006654	35.0	35.0	40.0	32.0	41.0
24	36.44375533001241	35.0	35.0	41.0	32.0	41.0
25	36.36943299648265	35.0	35.0	40.0	32.0	41.0
26	36.26313973874247	35.0	35.0	40.0	32.0	41.0
27	36.146290311364226	35.0	35.0	40.0	32.0	41.0
28	36.185641358334216	36.0	35.0	40.0	32.0	41.0
29	36.17220606697241	36.0	35.0	40.0	32.0	41.0
30	36.11175686359194	36.0	35.0	40.0	32.0	41.0
31	35.909197176603215	36.0	35.0	40.0	31.0	41.0
32	35.659904947349546	35.0	35.0	40.0	31.0	41.0
33	35.4605556206239	35.0	35.0	40.0	30.0	41.0
34	35.249467913782816	35.0	35.0	40.0	30.0	41.0
35	35.06707397416705	35.0	35.0	40.0	29.0	41.0
36	34.84684792673955	35.0	34.0	40.0	27.0	41.0
37	34.72862093134761	35.0	34.0	40.0	27.0	41.0
38	34.71211207574931	35.0	34.0	40.0	27.0	41.0
39	34.771581288865626	35.0	34.0	40.0	27.0	41.0
40	34.62214009377162	35.0	34.0	40.0	26.0	41.0
41	34.576208197147324	35.0	34.0	40.0	26.0	41.0
42	34.5494744104503	35.0	34.0	40.0	26.0	41.0
43	34.482538147333436	35.0	34.0	40.0	26.0	41.0
44	34.38622505188184	35.0	34.0	40.0	26.0	41.0
45	34.20923341153735	35.0	34.0	40.0	24.0	41.0
46	33.98223527452538	35.0	34.0	39.0	24.0	41.0
47	33.908999531507924	35.0	34.0	39.0	23.0	41.0
48	34.04279519576015	35.0	34.0	39.0	24.0	41.0
49	34.12380452168057	35.0	34.0	39.0	24.0	41.0
50	34.02171122221531	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	8.0
14	22.0
15	40.0
16	103.0
17	296.0
18	741.0
19	1565.0
20	2792.0
21	4519.0
22	7034.0
23	10401.0
24	15287.0
25	23129.0
26	32392.0
27	41828.0
28	44065.0
29	43511.0
30	48573.0
31	58322.0
32	73436.0
33	99703.0
34	210952.0
35	508124.0
36	103793.0
37	124156.0
38	209416.0
39	521525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.69396304036718	23.164782938104146	30.831262329942867	15.30999169158581
2	29.01388822803852	26.477031077861184	31.595673036450876	12.913407657649415
3	26.85415805019453	26.363247894530723	32.940940717451696	13.841653337823049
4	24.632023263559734	27.97465018648181	31.783939139951027	15.60938741000743
5	22.698669921710582	32.702805828334256	30.90414395883126	13.694380291123906
6	20.60655998711647	39.714906100279265	28.06903486971894	11.609499042885325
7	85.79274898706889	4.995022271674164	7.557591584711054	1.6546371565458957
8	88.14870597363999	3.386410801670467	6.710233989832258	1.7546492348572746
9	85.28706120043775	4.189344001288353	8.104363930502128	2.419230867771771
10	59.949280242444644	22.299902641490096	10.799840419886024	6.950976696179227
11	51.61378135328329	17.538119882730577	14.885146239070043	15.962952524916094
12	45.423921278690564	19.61041955661617	22.30068041154101	12.664978753152257
13	17.048765267168587	46.249318307425966	28.063178718747366	8.638737706658077
14	9.018243740323625	45.919635308198245	31.949604160795264	13.112516790682863
15	7.987698422865341	17.522518730532873	64.02484106040254	10.46494178619925
16	16.199852132187967	10.942538348638628	56.70122100747758	16.156388511695834
17	14.198146528217498	15.602433230728687	28.33251591226022	41.866904328793595
18	16.59029269774575	18.91436111222947	43.73675503354477	20.758591156480012
19	27.391871662451457	17.838430624741505	36.09887012887192	18.670827583935115
20	29.054744031301126	16.885525058836016	28.78357679060966	25.276154119253196
21	17.467114052200266	24.496233762906407	35.69786104085763	22.338791144035692
22	25.371682581976962	21.481688547930766	26.456671802999082	26.68995706709319
23	17.704196664190004	35.87066324569847	22.324608278401417	24.100531811710106
24	14.792225593575804	23.25893886544395	41.01469710889147	20.93413843208878
25	10.560012737128362	28.90957553885739	39.00585432092439	21.524557403089855
26	14.928243850126458	37.38571355369542	28.88450389251035	18.80153870366778
27	15.973612549731534	41.75440217848816	27.05198615020295	15.219999121577354
28	12.647135793160746	29.691920707715845	39.165708941976526	18.495234557146883
29	11.363677955617696	21.5821581380368	45.51052826141858	21.543635644926926
30	13.301926673669648	30.098968951419568	36.19485610339034	20.40424827152044
31	29.707842118169808	28.08248571648177	23.70149002441283	18.508182140935595
32	36.30712949779845	24.264778545990914	27.280055779838	12.148036176372628
33	37.65436447951628	22.53899830537631	23.395734892045517	16.410902323061887
34	20.957151275359877	28.513873587661088	28.235294655896233	22.293680481082802
35	17.597093153061486	27.73866560279924	33.72790675543615	20.936334488703118
36	38.58906107599454	22.18991680605526	24.461417115333234	14.759605002616969
37	21.467963194091144	38.00770083852762	26.10470797937171	14.41962798800953
38	16.658141696892944	41.877930363044754	19.659922332797738	21.804005607264557
39	16.660612260584077	38.324482005146095	26.34243110787396	18.672474626395868
40	21.425551850726713	25.405355450063503	22.769584249881962	30.399508449327822
41	12.67737732278738	23.50137436543114	23.204220454803327	40.617027856978154
42	18.11508800696882	26.875935611620065	21.51101505396809	33.49796132744302
43	18.466914577057796	27.66500620385994	26.424783230911693	27.443295988170576
44	20.233504869755542	31.976185596064667	27.790867698569272	19.999441835610522
45	19.297298484354926	39.57682904065267	18.54876343712141	22.577109037870997
46	21.892122378914927	32.25298023183038	25.557203614709188	20.297693774545507
47	23.830142341069553	23.42638818228734	26.23642562505261	26.507043851590495
48	22.38019596145189	19.33312165787634	29.486269156018842	28.80041322465293
49	18.147205334953533	21.02010489830428	36.11117719614811	24.72151257059407
50	19.409571878763035	33.534059008041226	24.61784039792546	22.43852871527028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2619.0
1	3055.0
2	3491.0
3	9485.5
4	15480.0
5	12472.5
6	9465.0
7	10752.0
8	12039.0
9	13225.5
10	14412.0
11	14100.5
12	13789.0
13	13354.5
14	12920.0
15	11465.0
16	10010.0
17	9094.5
18	8179.0
19	7633.0
20	7087.0
21	6710.0
22	6333.0
23	6167.5
24	6002.0
25	5648.5
26	5295.0
27	6863.5
28	8432.0
29	10461.0
30	12490.0
31	13895.5
32	15301.0
33	18490.5
34	21680.0
35	27427.5
36	33175.0
37	33345.0
38	33515.0
39	59097.0
40	84679.0
41	143014.0
42	201349.0
43	249702.5
44	298056.0
45	329980.0
46	361904.0
47	336005.5
48	310107.0
49	272206.0
50	234305.0
51	193711.0
52	153117.0
53	124742.5
54	96368.0
55	78495.5
56	60623.0
57	55421.5
58	50220.0
59	40153.0
60	30086.0
61	25897.0
62	21708.0
63	17518.0
64	13328.0
65	10712.0
66	8096.0
67	6174.0
68	4252.0
69	3663.0
70	3074.0
71	2220.5
72	1367.0
73	1065.5
74	764.0
75	518.0
76	272.0
77	236.5
78	201.0
79	142.5
80	84.0
81	60.5
82	37.0
83	24.5
84	12.0
85	11.0
86	10.0
87	5.0
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2185736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.235239505024548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81219331194839	10.563334939049554
2	9.237905338608018	2.44531779362444
3	3.1728561989816857	1.259805351255733
4	1.4809798163096246	0.7840449028386056
5	0.8059090500385665	0.5333199648263621
6	0.47753796268786775	0.37921975853492434
7	0.3596052702252739	0.3331623315090401
8	0.24394345463000774	0.2582920038168994
9	0.20086648144734012	0.23926643914384052
>10	1.7443751134082155	5.024989538860235
>50	0.5387328060207623	5.1655475994822115
>100	1.7344397565422804	56.35491729963512
>500	0.1819097768055638	13.94696915385294
>1k	0.007696182864850777	1.3775287365896682
>5k	6.99652987713707E-4	0.6225085326515223
>10k+	3.498264938568535E-4	0.7117756543288846
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	15373	0.703332881921696	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8376	0.3832118792022458	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	5069	0.23191272871014612	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	3023	0.1383058155239242	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06501242602034281	0.0
2	0.0	0.0	0.0	0.2855788622230681	0.0
3	0.0	0.0	0.0	0.3948326787864591	0.0
4	0.0	0.0	0.0	0.6091769545818891	0.0
5	0.0	0.0	0.0	1.1313809170000402	0.0
6	0.0	0.0	0.0	1.4630312169447728	0.0
7	0.0	0.0	0.0	1.7257344894351376	0.0
8	0.0	0.0	0.0	2.203056544797725	0.0
9	0.0	0.0	0.0	2.3576040290318683	0.0
10	0.0	0.0	0.0	2.847782165824235	0.0
11	0.0	0.0	0.0	3.092642478323091	0.0
12	0.0	0.0	0.0	3.417796110783736	0.0
13	0.0	0.0	0.0	3.526043401398888	0.0
14	0.0	0.0	0.0	3.5635593685605214	0.0
15	0.0	0.0	0.0	3.62811428278621	0.0
16	0.0	0.0	0.0	3.7483026312418333	0.0
17	0.0	0.0	0.0	3.900242298246449	0.0
18	0.0	0.0	0.0	4.187605456468668	0.0
19	0.0	0.0	0.0	4.267715771712595	0.0
20	4.5751179465406616E-5	0.0	0.0	4.360133154232716	0.0
21	4.5751179465406616E-5	0.0	0.0	4.495785401347646	0.0
22	4.5751179465406616E-5	0.0	0.0	4.605679734423553	0.0
23	4.5751179465406616E-5	0.0	0.0	4.74037120676971	0.0
24	4.5751179465406616E-5	0.0	0.0	4.82862523195848	0.0
25	4.5751179465406616E-5	0.0	0.0	4.913402167507878	0.0
26	4.5751179465406616E-5	0.0	0.0	5.00312023043954	0.0
27	4.5751179465406616E-5	0.0	0.0	5.0705574689715505	0.0
28	4.5751179465406616E-5	0.0	0.0	5.138269214580352	0.0
29	4.5751179465406616E-5	0.0	0.0	5.21796776920909	0.0
30	4.5751179465406616E-5	0.0	0.0	5.342639733252323	0.0
31	4.5751179465406616E-5	0.0	0.0	5.466762683141972	0.0
32	4.5751179465406616E-5	0.0	0.0	5.5797680964215255	0.0
33	4.5751179465406616E-5	0.0	0.0	5.670080924686238	0.0
34	4.5751179465406616E-5	0.0	0.0	5.761674785975982	0.0
35	4.5751179465406616E-5	0.0	0.0	5.922078421181698	0.0
36	4.5751179465406616E-5	0.0	0.0	6.040345220099774	0.0
37	9.150235893081323E-5	0.0	0.0	6.1739386641387615	0.0
38	9.150235893081323E-5	0.0	0.0	6.2789376210118695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	40	8.3182385E-9	44.0	1
ACGCGAA	25	4.4441622E-5	44.0	30
TATCATC	55	1.8189894E-12	44.0	41
TATTTCG	35	1.4468787E-7	44.0	1
TCTTGTC	30	2.5287682E-6	44.0	34
AACCGAG	80	0.0	44.0	1
ATCTCGA	25	4.4441622E-5	44.0	12
ACCGGTT	20	7.858041E-4	44.0	34
AGTCCAT	25	4.4441622E-5	44.0	27
GTACCAT	50	2.7284841E-11	44.0	38
AAATCCG	35	1.4468787E-7	44.0	21
CGTATTG	20	7.858041E-4	44.0	38
GCGACGG	165	0.0	44.0	2
ACGCCAT	30	2.5287682E-6	44.0	34
GCGACAG	35	1.4468787E-7	44.0	1
TACTTGC	95	0.0	44.0	19
ATCTACG	30	2.5287682E-6	44.0	1
GTCGACG	25	4.4441622E-5	44.0	1
GTCTGGC	25	4.4441622E-5	44.0	21
AGTCAAT	95	0.0	44.0	14
>>END_MODULE