FastQCFastQC Report
Thu 26 May 2016
SRR1545841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545841_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2978236
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208400.6997430693873824No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA100190.3364071886848457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAT408.3200575E-944.00000428
CGTCTAC207.858501E-444.00000429
ATCGTAC302.5290792E-644.00000410
GCGTAAT207.858501E-444.0000045
TCGCAAA254.4445515E-544.039
CGCGATT351.4471152E-744.014
CGTTTTT118400.041.5658761
CTATGCG700.040.8571431
CGTAAGG5550.040.828832
TCGACAG603.6379788E-1240.3333361
CGTTAGG5850.040.239322
AATCTCG1100.040.018
CGGTCTA5600.039.67857431
GTTTACG1000.039.6000021
ATTCACG501.3496901E-939.6000021
CACAACG2000.039.60000212
CTAGACG1350.039.111111
CGACGGT5700.038.98245228
CTACGCG404.127487E-738.5000041
TACACCG404.127487E-738.50000415
Produced by FastQC (version 0.11.5)