##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545841_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2978236
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.676291939255318	31.0	31.0	33.0	30.0	34.0
2	32.09942227546776	33.0	31.0	34.0	30.0	34.0
3	32.13733364313641	33.0	31.0	34.0	30.0	34.0
4	35.83168996681257	37.0	35.0	37.0	35.0	37.0
5	35.807656948609846	37.0	35.0	37.0	35.0	37.0
6	35.8588701499814	37.0	35.0	37.0	35.0	37.0
7	36.084936183700684	37.0	35.0	37.0	35.0	37.0
8	36.03738118805897	37.0	35.0	37.0	35.0	37.0
9	37.80188138213359	39.0	38.0	39.0	35.0	39.0
10	37.52245591014278	39.0	37.0	39.0	35.0	39.0
11	37.296869690649096	39.0	37.0	39.0	34.0	39.0
12	36.59681166972664	39.0	35.0	39.0	33.0	39.0
13	36.326049379565625	39.0	35.0	39.0	33.0	39.0
14	37.269706631710854	39.0	35.0	41.0	33.0	41.0
15	37.471205102617795	40.0	35.0	41.0	33.0	41.0
16	37.55612785554939	40.0	35.0	41.0	33.0	41.0
17	37.48925370588496	39.0	35.0	41.0	33.0	41.0
18	37.397990958406254	39.0	35.0	41.0	33.0	41.0
19	37.33637764099286	39.0	35.0	41.0	33.0	41.0
20	37.16595830552045	39.0	35.0	41.0	33.0	41.0
21	37.065044543145675	39.0	35.0	41.0	32.0	41.0
22	37.0947201632107	39.0	35.0	41.0	33.0	41.0
23	37.09673545011208	39.0	35.0	41.0	33.0	41.0
24	37.062451733173596	39.0	35.0	41.0	33.0	41.0
25	36.973307353749	38.0	35.0	41.0	32.0	41.0
26	36.84379108975917	38.0	35.0	41.0	32.0	41.0
27	36.75089415345191	38.0	35.0	41.0	32.0	41.0
28	36.72838418446356	38.0	35.0	41.0	32.0	41.0
29	36.64973427223363	38.0	35.0	41.0	32.0	41.0
30	36.546258254886446	38.0	35.0	41.0	31.0	41.0
31	36.356033571550405	38.0	35.0	41.0	31.0	41.0
32	36.19902586631818	38.0	35.0	41.0	30.0	41.0
33	36.00819814145017	38.0	35.0	41.0	30.0	41.0
34	35.79292742415309	38.0	35.0	41.0	29.0	41.0
35	35.59071880133072	38.0	35.0	41.0	29.0	41.0
36	35.440837462175594	38.0	35.0	40.0	27.0	41.0
37	35.35425332310804	38.0	35.0	40.0	27.0	41.0
38	35.23397440632643	38.0	34.0	40.0	27.0	41.0
39	35.168099170112775	38.0	34.0	40.0	26.0	41.0
40	34.991476498168716	38.0	34.0	40.0	24.0	41.0
41	34.87029167601224	37.0	34.0	40.0	24.0	41.0
42	34.867646486040734	37.0	34.0	40.0	24.0	41.0
43	34.79784476448475	37.0	34.0	40.0	24.0	41.0
44	34.66703075243198	37.0	34.0	40.0	24.0	41.0
45	34.568352541571585	37.0	34.0	40.0	23.0	41.0
46	34.548068051020806	36.0	34.0	40.0	23.0	41.0
47	34.46273868155512	36.0	34.0	40.0	23.0	41.0
48	34.40409692180204	36.0	34.0	40.0	23.0	41.0
49	34.346748209342714	36.0	34.0	40.0	24.0	41.0
50	34.18553801646344	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	8.0
12	16.0
13	17.0
14	42.0
15	79.0
16	222.0
17	554.0
18	1203.0
19	2446.0
20	4276.0
21	6968.0
22	10203.0
23	14986.0
24	21559.0
25	31214.0
26	44204.0
27	54913.0
28	57415.0
29	59596.0
30	65495.0
31	75893.0
32	93679.0
33	122729.0
34	227294.0
35	436649.0
36	175993.0
37	230063.0
38	389091.0
39	851426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.1518509614416	23.19614698096457	29.828764409536383	14.823237648057441
2	32.38876972812094	25.041836845703298	29.357042222308777	13.212351203866987
3	29.74975791038722	24.473648159514557	32.37191411291785	13.404679817180371
4	26.418356369340778	26.61659452105206	31.887969925821864	15.077079183785303
5	23.324343671891683	31.16982670278648	31.568821275412695	13.937008349909142
6	21.041750888781145	39.76286634101528	28.590313192104322	10.60506957809925
7	85.39558315727834	4.293816876835818	8.483478139408698	1.8271218264771496
8	86.19565407173911	3.760984690266319	8.152711873740026	1.8906493642545454
9	81.78686981152602	5.221345789923968	9.92607704694994	3.0657073516000746
10	45.67082662354495	27.834630969473206	16.14086324925224	10.353679157729609
11	41.97864104792232	21.022007658224535	23.802008974439904	13.197342319413236
12	37.75164224729001	21.558902652442587	26.457305599690557	14.232149500576853
13	23.499514477697538	33.54012240802945	27.535124818852502	15.42523829542051
14	17.16193746902529	35.265539735601884	31.66508631283753	15.907436482535301
15	16.344272247061685	23.346538017806516	45.250779320376225	15.05841041475558
16	19.884858016624605	20.041393630323455	41.69041674333398	18.383331609717967
17	19.839965670954214	19.932134323807784	30.423008787752217	29.804891217485785
18	22.586289333686118	22.872935522906847	35.168032352036576	19.372742791370463
19	27.89637893034669	24.13361466317646	27.77039831631879	20.199608090158065
20	28.596625653574804	23.9706994341617	28.610325038042657	18.822349874220848
21	23.218442057647547	26.065227873143698	30.873040282905723	19.843289786303036
22	22.2269491067867	23.299798941386783	30.190488597948583	24.282763353877932
23	19.580382481442037	28.163483350547104	29.286094184611294	22.97003998339957
24	20.24698512810939	23.55666240015902	37.13382686932802	19.06252560240357
25	20.01741970750471	24.229443200605996	34.96395181577282	20.789185276116466
26	18.179083188840643	30.659826823663405	29.548027758713545	21.613062228782407
27	18.13032949705799	29.61884148872017	31.941457963707375	20.309371050514464
28	16.392723746539897	26.42960463845041	37.44528640443538	19.732385210574314
29	17.874238307508204	24.771072540926912	36.41833622318715	20.936352928377737
30	20.515634086754712	27.39060974348574	33.17819675808096	18.91555941167859
31	26.89548444112555	25.35131534237045	28.172381235066663	19.580818981437336
32	29.095511571279104	25.569195993870196	28.50281173150818	16.832480703342515
33	26.868186402957996	26.108575680369185	27.29917978293191	19.72405813374091
34	19.909570631743083	26.907102056385057	31.110496280348503	22.072831031523357
35	20.577314893782763	26.63482679008648	31.351813623903546	21.43604469222721
36	27.289140283040027	26.240096486645115	27.983981121710972	18.486782108603887
37	20.60300123965999	31.106198434240937	29.784006371556853	18.506793954542218
38	21.299823116771137	31.351007777758376	26.029569181220026	21.31959992425046
39	21.101148465064554	29.71450214153613	27.76821581634229	21.41613357705702
40	23.662496860557727	26.552831944815658	27.234779245163914	22.5498919494627
41	18.837459489442743	25.25313642035084	28.419037309333444	27.49036678087297
42	21.88392726432694	25.611066416496207	26.884907710470223	25.62009860870663
43	21.729607727527302	26.110959641881976	28.060335043965623	24.099097586625103
44	20.14652297534514	28.221369965308323	29.949204831316255	21.682902228030283
45	18.876576604406097	33.00030622153516	25.93521802838996	22.18789914566878
46	21.942250379083458	30.169301559715212	27.517866280576825	20.3705817806245
47	20.769744237864295	27.725841739875552	29.19100433948149	22.313409682778666
48	21.454109076648056	26.04706275795471	31.813261272780263	20.685566892616972
49	21.255065078791606	25.476456533330467	31.90398611795707	21.364492269920852
50	19.95234763128241	30.563125286243263	28.58957449980458	20.89495258266974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2038.0
1	5199.0
2	8360.0
3	24474.5
4	40589.0
5	28665.5
6	16742.0
7	16263.0
8	15784.0
9	16057.5
10	16331.0
11	15805.5
12	15280.0
13	14138.0
14	12996.0
15	12419.0
16	11842.0
17	11164.0
18	10486.0
19	11249.5
20	12013.0
21	12786.5
22	13560.0
23	15689.5
24	17819.0
25	18691.0
26	19563.0
27	23987.0
28	28411.0
29	32924.0
30	37437.0
31	47462.5
32	57488.0
33	63950.5
34	70413.0
35	79688.5
36	88964.0
37	98538.0
38	108112.0
39	125753.0
40	143394.0
41	178707.0
42	214020.0
43	244056.0
44	274092.0
45	279188.0
46	284284.0
47	272659.5
48	261035.0
49	245048.5
50	229062.0
51	217068.0
52	205074.0
53	193586.0
54	182098.0
55	168231.0
56	154364.0
57	141419.5
58	128475.0
59	114943.0
60	101411.0
61	88897.0
62	76383.0
63	62593.5
64	48804.0
65	39015.5
66	29227.0
67	22610.0
68	15993.0
69	13155.0
70	10317.0
71	8546.0
72	6775.0
73	5451.5
74	4128.0
75	3097.5
76	2067.0
77	1729.5
78	1392.0
79	1081.0
80	770.0
81	570.5
82	371.0
83	290.0
84	209.0
85	180.5
86	152.0
87	108.0
88	64.0
89	41.5
90	19.0
91	15.0
92	11.0
93	8.0
94	5.0
95	7.5
96	10.0
97	5.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2978236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.789913149714074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66588121917812	12.579173454462603
2	7.98957796510835	2.523094843438603
3	2.6571348432611765	1.2586778520651927
4	1.3233572303516934	0.835827829331976
5	0.8043683580720776	0.6350453257168108
6	0.5947197241583693	0.5634343675729536
7	0.42118392915118347	0.4655320362946759
8	0.3297979051635506	0.41659842235920824
9	0.29133910430502596	0.41401972386826646
>10	3.2602923798778023	12.151002031631768
>50	1.0192351293061854	11.508815386535689
>100	1.6202882190285313	53.11819178449419
>500	0.019594182702342804	1.8977533795047161
>1k	0.0027991689574775433	0.5836599710838344
>5k	0.0	0.0
>10k+	4.306413780734682E-4	1.0491735916395435
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20840	0.6997430693873824	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	10019	0.3364071886848457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03982223034037598	0.0
2	0.0	0.0	0.0	0.1484435753244538	0.0
3	0.0	0.0	0.0	0.2084455362167404	0.0
4	0.0	0.0	0.0	0.298062342943944	0.0
5	0.0	0.0	0.0	0.5372979172906378	0.0
6	0.0	0.0	0.0	0.7172366461220668	0.0
7	0.0	0.0	0.0	0.8472129139530917	0.0
8	0.0	0.0	0.0	1.1132764495493306	0.0
9	0.0	0.0	0.0	1.212999910013847	0.0
10	3.357692271532545E-5	0.0	0.0	1.4520675997469643	0.0
11	3.357692271532545E-5	0.0	0.0	1.6577262513783326	0.0
12	3.357692271532545E-5	0.0	0.0	1.859053479979424	0.0
13	3.357692271532545E-5	0.0	0.0	1.9294642869134615	0.0
14	3.357692271532545E-5	0.0	0.0	1.9599185558162617	0.0
15	3.357692271532545E-5	0.0	0.0	2.006590478390564	0.0
16	3.357692271532545E-5	0.0	0.0	2.104366477337592	0.0
17	3.357692271532545E-5	0.0	0.0	2.2165133992067787	0.0
18	3.357692271532545E-5	0.0	0.0	2.387520666595931	0.0
19	3.357692271532545E-5	0.0	0.0	2.4590395119795745	0.0
20	3.357692271532545E-5	0.0	0.0	2.547111780261873	0.0
21	3.357692271532545E-5	0.0	0.0	2.662515663634447	0.0
22	3.357692271532545E-5	0.0	0.0	2.7838962392503483	0.0
23	3.357692271532545E-5	0.0	0.0	2.9113542378777235	0.0
24	3.357692271532545E-5	0.0	0.0	3.003119967658708	0.0
25	3.357692271532545E-5	0.0	0.0	3.0878009667467587	0.0
26	3.357692271532545E-5	0.0	0.0	3.1773506196285317	0.0
27	3.357692271532545E-5	0.0	0.0	3.280230310828289	0.0
28	3.357692271532545E-5	0.0	0.0	3.382169848192017	0.0
29	3.357692271532545E-5	3.357692271532545E-5	0.0	3.5073781929974657	0.0
30	3.357692271532545E-5	3.357692271532545E-5	0.0	3.635239114697425	0.0
31	3.357692271532545E-5	3.357692271532545E-5	0.0	3.7519524980558963	0.0
32	3.357692271532545E-5	3.357692271532545E-5	0.0	3.8621855353303096	0.0
33	3.357692271532545E-5	3.357692271532545E-5	0.0	3.9742317264313507	0.0
34	3.357692271532545E-5	3.357692271532545E-5	0.0	4.100279494304682	0.0
35	3.357692271532545E-5	3.357692271532545E-5	0.0	4.249159569624435	0.0
36	3.357692271532545E-5	3.357692271532545E-5	0.0	4.371782491380804	0.0
37	3.357692271532545E-5	3.357692271532545E-5	0.0	4.501993797670836	0.0
38	3.357692271532545E-5	3.357692271532545E-5	0.0	4.658663719060544	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAT	40	8.3200575E-9	44.000004	28
CGTCTAC	20	7.858501E-4	44.000004	29
ATCGTAC	30	2.5290792E-6	44.000004	10
GCGTAAT	20	7.858501E-4	44.000004	5
TCGCAAA	25	4.4445515E-5	44.0	39
CGCGATT	35	1.4471152E-7	44.0	14
CGTTTTT	11840	0.0	41.565876	1
CTATGCG	70	0.0	40.857143	1
CGTAAGG	555	0.0	40.82883	2
TCGACAG	60	3.6379788E-12	40.333336	1
CGTTAGG	585	0.0	40.23932	2
AATCTCG	110	0.0	40.0	18
CGGTCTA	560	0.0	39.678574	31
GTTTACG	100	0.0	39.600002	1
ATTCACG	50	1.3496901E-9	39.600002	1
CACAACG	200	0.0	39.600002	12
CTAGACG	135	0.0	39.11111	1
CGACGGT	570	0.0	38.982452	28
CTACGCG	40	4.127487E-7	38.500004	1
TACACCG	40	4.127487E-7	38.500004	15
>>END_MODULE