FastQCFastQC Report
Thu 26 May 2016
SRR1545840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545840_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences646068
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.5380238612653776No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA16370.25337890129212404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG302.5259887E-644.0000041
TTCGTAA302.5259887E-644.00000418
CGTAAGT302.5259887E-644.00000420
AGTAGCG600.044.0000041
AATACGG302.5259887E-644.0000042
AACGTCG207.853919E-444.01
AGCCGTA207.853919E-444.012
CGAACAC207.853919E-444.022
ACGCCTA207.853919E-444.043
CAAGTCG207.853919E-444.034
CGACGGA207.853919E-444.028
TCCGATA207.853919E-444.014
ACGTTCA207.853919E-444.041
TTGTACG254.4406694E-544.01
TAACGGA207.853919E-444.03
GCGCACC207.853919E-444.036
GACCCCG351.4447687E-744.09
AATGCCG254.4406694E-544.018
CTAACCG207.853919E-444.017
CCGTACT207.853919E-444.014
Produced by FastQC (version 0.11.5)