##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545840_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	646068
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76167059814137	31.0	31.0	33.0	30.0	34.0
2	32.20367825058663	33.0	31.0	34.0	31.0	34.0
3	32.249622330776326	34.0	31.0	34.0	30.0	34.0
4	35.90986862064055	37.0	35.0	37.0	35.0	37.0
5	35.91421800801154	37.0	35.0	37.0	35.0	37.0
6	35.96292805091724	37.0	35.0	37.0	35.0	37.0
7	36.137414327903564	37.0	35.0	37.0	35.0	37.0
8	36.090442492121575	37.0	35.0	37.0	35.0	37.0
9	37.872788932434354	39.0	38.0	39.0	35.0	39.0
10	37.6271073633116	39.0	37.0	39.0	35.0	39.0
11	37.408018970139366	39.0	37.0	39.0	35.0	39.0
12	36.61773838048007	39.0	35.0	39.0	33.0	39.0
13	36.33414129782005	39.0	35.0	39.0	33.0	39.0
14	37.318646024876635	39.0	35.0	41.0	33.0	41.0
15	37.526484828222415	40.0	35.0	41.0	33.0	41.0
16	37.61760062408291	39.0	35.0	41.0	33.0	41.0
17	37.54462533355622	39.0	35.0	41.0	33.0	41.0
18	37.47032820074667	39.0	35.0	41.0	33.0	41.0
19	37.40346526990967	39.0	35.0	41.0	33.0	41.0
20	37.25222577190017	39.0	35.0	41.0	33.0	41.0
21	37.134111270021116	39.0	35.0	41.0	33.0	41.0
22	37.13568850337735	39.0	35.0	41.0	33.0	41.0
23	37.146193589529275	39.0	35.0	41.0	33.0	41.0
24	37.10407418414161	38.0	35.0	41.0	33.0	41.0
25	36.995276039054716	38.0	35.0	41.0	33.0	41.0
26	36.87301646266337	38.0	35.0	41.0	33.0	41.0
27	36.79284533516596	38.0	35.0	41.0	32.0	41.0
28	36.77003194710154	38.0	35.0	41.0	32.0	41.0
29	36.742003937666006	38.0	35.0	41.0	32.0	41.0
30	36.64408545230533	38.0	35.0	41.0	32.0	41.0
31	36.46835472427051	38.0	35.0	41.0	31.0	41.0
32	36.267657584031404	38.0	35.0	41.0	31.0	41.0
33	36.116995424630225	38.0	35.0	41.0	30.0	41.0
34	35.948972863537584	38.0	35.0	41.0	30.0	41.0
35	35.74063101716847	38.0	35.0	41.0	30.0	41.0
36	35.57228496071621	38.0	35.0	40.0	29.0	41.0
37	35.50556597757512	38.0	35.0	40.0	29.0	41.0
38	35.425063615594645	38.0	35.0	40.0	28.0	41.0
39	35.38977940402558	38.0	35.0	40.0	28.0	41.0
40	35.21057690521741	37.0	35.0	40.0	27.0	41.0
41	35.122541280484405	37.0	34.0	40.0	27.0	41.0
42	35.087371607942195	37.0	34.0	40.0	27.0	41.0
43	35.028037296383665	37.0	34.0	40.0	27.0	41.0
44	34.88686949361367	37.0	34.0	40.0	26.0	41.0
45	34.795165524372045	36.0	34.0	40.0	26.0	41.0
46	34.779784790455494	36.0	34.0	40.0	26.0	41.0
47	34.71949547106497	36.0	34.0	40.0	26.0	41.0
48	34.658210900400576	36.0	34.0	40.0	25.0	41.0
49	34.618160936619674	36.0	34.0	40.0	26.0	41.0
50	34.45070178371317	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	6.0
14	17.0
15	23.0
16	26.0
17	84.0
18	233.0
19	401.0
20	742.0
21	1294.0
22	1932.0
23	2866.0
24	4221.0
25	5993.0
26	8753.0
27	10761.0
28	11487.0
29	11503.0
30	12990.0
31	15423.0
32	19116.0
33	25909.0
34	51675.0
35	105923.0
36	36178.0
37	47467.0
38	83010.0
39	188028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.551836648773815	23.500931790461685	29.17943622033594	14.767795340428561
2	33.092027464601244	24.47915699276237	29.096472817102843	13.332342725533536
3	29.390714290136643	23.975030492146338	33.09527789644434	13.538977321272682
4	26.209934557972225	27.099469405697235	32.47970801835101	14.210888017979531
5	23.500622225524246	31.380133360575048	30.994879795934793	14.12436461796591
6	21.538909216986447	40.253967074673255	28.05973984162658	10.14738386671372
7	86.34029235312691	4.328646520180538	7.6300017954766375	1.701059331215909
8	88.0981568503625	3.0238303088838943	7.166583084133558	1.7114297566200463
9	83.57185311762848	5.077484103840463	8.924292798900426	2.426369979630627
10	48.01677222831033	27.47419776246463	14.237665385067826	10.27136462415721
11	44.273203439885584	19.841255100082343	22.908888847613564	12.976652612418507
12	39.55373118619093	21.466780586563644	25.45738219506306	13.522106032182371
13	22.73259161574323	36.77136152850783	25.771745389030258	14.724301466718673
14	16.538816347505218	37.74680064637159	30.60978101376326	15.104601992359937
15	15.743234458292315	22.942012295919316	46.759938582316416	14.554814663471957
16	18.00894023539318	19.188382647027865	43.213562659038985	19.589114458539967
17	18.489539800764007	19.48927976621656	30.24898307918052	31.772197353838916
18	22.515431812131233	23.150349498814364	34.58939306698366	19.744825622070742
19	27.65869846517704	24.229183305782055	27.92972256790307	20.182395661137836
20	29.70987574063411	22.82886631128612	27.61303763690509	19.848220311174675
21	22.723923797495	26.50448559594346	30.099617996867202	20.671972609694336
22	22.792647213606	23.177745995777535	28.37054303881325	25.659063751803217
23	19.96771237702533	29.02759461852313	27.15441718209229	23.850275822359258
24	19.9523889126222	23.3401128054632	39.33254703839225	17.374951243522354
25	18.511983258728186	25.791093197620064	35.669465133701095	20.027458409950654
26	17.566262374858375	31.93781459536767	29.679074029359136	20.816849000414816
27	18.048409764916386	31.938433725242543	30.818737346533183	19.194419163307888
28	15.936557761721676	27.434418668003985	37.95003002779893	18.678993542475407
29	17.02173764990682	24.119906882866818	37.6915433050391	21.166812162187263
30	19.576267513636335	29.582799333816258	32.03904232990954	18.801890822637866
31	27.865487843384905	26.3035779515469	27.287220540252726	18.543713664815467
32	28.58615501773807	25.456298717782026	28.300890927889945	17.656655336589957
33	26.768544487577163	27.18815976027291	26.702142808496937	19.34115294365299
34	19.396255502516762	26.764984490796635	30.814403437409066	23.024356569277536
35	21.237083402985444	26.05731904381582	32.086560547806116	20.61903700539262
36	28.305843966888933	26.0051573518577	27.359039605738094	18.32995907551527
37	20.124042670430974	31.826526000359095	29.712507042602326	18.3369242866076
38	20.60185615136487	31.572527969192098	26.479101271073635	21.346514608369397
39	20.75601949020846	30.947516360506945	28.176291040571584	20.120173108713015
40	24.585337766303237	26.805537497600874	26.743624510113484	21.865500225982405
41	19.1006209872645	25.252450206479814	28.56433068964877	27.08259811660692
42	22.51961093878663	25.264213674102415	26.098181615557497	26.11799377155346
43	22.352910219976845	26.555409028151832	27.313533559934868	23.77814719193645
44	19.425199824167116	29.74655918572039	29.037965043927265	21.79027594618523
45	18.24157828587703	35.629840821709166	24.422970956617572	21.705609935796232
46	21.052892265210474	31.278131713689582	27.91904257756149	19.74993344353845
47	22.024307038887546	27.371886550641726	28.645127138319804	21.95867927215092
48	22.734294222899138	25.118872935975777	31.619272274745075	20.52756056638001
49	21.386912832704915	24.608864701548445	32.511593206907015	21.492629258839628
50	19.524879734021805	32.68324696471579	27.981729477392474	19.810143823869932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	359.0
1	926.0
2	1493.0
3	4408.0
4	7323.0
5	5366.0
6	3409.0
7	3388.0
8	3367.0
9	3378.5
10	3390.0
11	3336.5
12	3283.0
13	3077.0
14	2871.0
15	2638.0
16	2405.0
17	2306.5
18	2208.0
19	2185.0
20	2162.0
21	2441.0
22	2720.0
23	2905.5
24	3091.0
25	3488.0
26	3885.0
27	4483.5
28	5082.0
29	6794.5
30	8507.0
31	10287.5
32	12068.0
33	13833.0
34	15598.0
35	17942.0
36	20286.0
37	21140.5
38	21995.0
39	28518.0
40	35041.0
41	43497.0
42	51953.0
43	58122.0
44	64291.0
45	63625.0
46	62959.0
47	57747.5
48	52536.0
49	52501.0
50	52466.0
51	49642.0
52	46818.0
53	42526.0
54	38234.0
55	34934.0
56	31634.0
57	28645.0
58	25656.0
59	22700.5
60	19745.0
61	16840.0
62	13935.0
63	12289.0
64	10643.0
65	8247.0
66	5851.0
67	4676.0
68	3501.0
69	2803.0
70	2105.0
71	1724.5
72	1344.0
73	1042.5
74	741.0
75	655.0
76	569.0
77	410.0
78	251.0
79	185.5
80	120.0
81	107.0
82	94.0
83	64.5
84	35.0
85	29.0
86	23.0
87	17.0
88	11.0
89	7.0
90	3.0
91	2.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	646068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.125778269362765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46238231875071	14.624025701756699
2	5.967802374525718	2.2827772994111095
3	2.369322310767235	1.3594539949316504
4	1.478519891275683	1.1311137442952421
5	1.1572536621942375	1.1066688472267512
6	0.8928637117507412	1.024602802542294
7	0.760088866719263	1.0176103790918696
8	0.5997502210432126	0.9176551795739032
9	0.5761462120087966	0.9917320231452161
>10	6.893393494399762	30.68789772674482
>50	2.2972551937938737	31.253487857060414
>100	0.5435942151804136	12.807193980228812
>500	0.0	0.0
>1k	0.00162752759036052	0.7957804639912249
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3476	0.5380238612653776	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1637	0.25337890129212404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01810954884006018	0.0
2	0.0	0.0	0.0	0.08791644223208703	0.0
3	0.0	0.0	0.0	0.12521901719323664	0.0
4	0.0	0.0	0.0	0.17165375780877554	0.0
5	0.0	0.0	0.0	0.29903972956407066	0.0
6	0.0	0.0	0.0	0.3911352984515562	0.0
7	0.0	0.0	0.0	0.454905675563563	0.0
8	0.0	0.0	0.0	0.6194394398112892	0.0
9	0.0	0.0	0.0	0.6876985085161315	0.0
10	0.0	0.0	0.0	0.8271575128314667	0.0
11	0.0	0.0	0.0	0.9591869586483156	0.0
12	0.0	0.0	0.0	1.0782146770928138	0.0
13	0.0	0.0	0.0	1.1232563754898865	0.0
14	0.0	0.0	0.0	1.1436876613607236	0.0
15	0.0	0.0	0.0	1.1727867654797948	0.0
16	0.0	0.0	0.0	1.2331519282799952	0.0
17	0.0	0.0	0.0	1.3088405554833238	0.0
18	0.0	0.0	0.0	1.423224799866268	0.0
19	0.0	0.0	0.0	1.4719812775125838	0.0
20	0.0	0.0	0.0	1.527238618845075	0.0
21	0.0	0.0	0.0	1.6016889862986559	0.0
22	0.0	0.0	0.0	1.673972399190178	0.0
23	0.0	0.0	0.0	1.7555427602048082	0.0
24	0.0	0.0	0.0	1.815288793130135	0.0
25	0.0	0.0	0.0	1.867450485088257	0.0
26	0.0	0.0	0.0	1.9213147842022822	0.0
27	0.0	0.0	0.0	1.9883355931573765	0.0
28	0.0	0.0	0.0	2.047617278676548	0.0
29	0.0	0.0	0.0	2.115102435037798	0.0
30	0.0	0.0	0.0	2.195124971365243	0.0
31	0.0	0.0	0.0	2.270349251162416	0.0
32	0.0	0.0	0.0	2.3378344075236663	0.0
33	0.0	0.0	0.0	2.405629128822353	0.0
34	0.0	0.0	0.0	2.480234278744652	0.0
35	0.0	0.0	0.0	2.5748063671316332	0.0
36	0.0	0.0	0.0	2.656067163208826	0.0
37	0.0	0.0	0.0	2.737173176817301	0.0
38	0.0	0.0	0.0	2.8289591807673498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	30	2.5259887E-6	44.000004	1
TTCGTAA	30	2.5259887E-6	44.000004	18
CGTAAGT	30	2.5259887E-6	44.000004	20
AGTAGCG	60	0.0	44.000004	1
AATACGG	30	2.5259887E-6	44.000004	2
AACGTCG	20	7.853919E-4	44.0	1
AGCCGTA	20	7.853919E-4	44.0	12
CGAACAC	20	7.853919E-4	44.0	22
ACGCCTA	20	7.853919E-4	44.0	43
CAAGTCG	20	7.853919E-4	44.0	34
CGACGGA	20	7.853919E-4	44.0	28
TCCGATA	20	7.853919E-4	44.0	14
ACGTTCA	20	7.853919E-4	44.0	41
TTGTACG	25	4.4406694E-5	44.0	1
TAACGGA	20	7.853919E-4	44.0	3
GCGCACC	20	7.853919E-4	44.0	36
GACCCCG	35	1.4447687E-7	44.0	9
AATGCCG	25	4.4406694E-5	44.0	18
CTAACCG	20	7.853919E-4	44.0	17
CCGTACT	20	7.853919E-4	44.0	14
>>END_MODULE