FastQCFastQC Report
Thu 26 May 2016
SRR1545839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545839_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2195639
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117750.53629034645495No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA74650.3399921389627347No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA59330.2702174628889358No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA28510.12984830384229828No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG207.858049E-444.00000441
TCTACGA207.858049E-444.00000429
GCGATAT750.044.0000049
GCGTATC207.858049E-444.00000440
CGAAGTA207.858049E-444.00000430
ACGGTAC650.044.00000413
CGGAATA551.8189894E-1244.011
GTCGAAT351.446897E-744.019
CGAAATA302.5287754E-644.031
CGAGTAT254.4441687E-544.042
ACGCATA302.5287754E-644.023
TAGCGAA351.446897E-744.034
CACGCGT254.4441687E-544.026
CCTACGC302.5287754E-644.032
CGTCTAC351.446897E-744.010
TAACGGC254.4441687E-544.034
GCGCAAC551.8189894E-1244.015
CGGATAC254.4441687E-544.014
TACGACT254.4441687E-544.032
TCGCTAC254.4441687E-544.029
Produced by FastQC (version 0.11.5)