FastQCFastQC Report
Thu 26 May 2016
SRR1545838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545838_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2367998
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142230.6006339532381362No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA107160.45253416599169427No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA46830.19776199135303324No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA43930.18551535938797245No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA42120.17787177185115866No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA32030.1352619385658265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC207.858173E-444.00000421
CTCCGTC207.858173E-444.00000414
ACGCATG650.044.0000041
CGACATA207.858173E-444.00000429
GAACGTA207.858173E-444.00000420
GAGTTAC207.858173E-444.00000439
ACGTAAG650.044.0000041
ACGTAAC207.858173E-444.00000440
TCGACGA207.858173E-444.00000425
ATCGCTA207.858173E-444.00000428
TCGAATC408.3182385E-944.00000415
TCGGTAT207.858173E-444.00000416
ATACCGG800.044.0000042
TGCAACG408.3182385E-944.0000041
TCACGCA302.528859E-644.013
AACGTAG502.7284841E-1144.01
ACACGGC351.4469515E-744.038
CTCGTTA502.7284841E-1144.035
ACGTTGA551.8189894E-1244.035
CTATACG502.7284841E-1144.01
Produced by FastQC (version 0.11.5)