FastQCFastQC Report
Thu 26 May 2016
SRR1545837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545837_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2371197
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151830.6403095145616328No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA92600.3905200622301732No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA60690.25594668009448396No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA51010.21512341656977468No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA26320.11099879090602763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA302.528859E-644.00000411
TACCCGT302.528859E-644.00000434
CTAAGCG600.044.0000041
AGTGCGT302.528859E-644.00000411
TACGACT302.528859E-644.00000419
TCGAGTA302.528859E-644.0000049
CACGTTA302.528859E-644.00000432
AATACGC302.528859E-644.00000423
AGTCGTA600.044.00000414
CGAACCG207.8581774E-444.027
TAGCGAA207.8581774E-444.01
CGACACT351.4469515E-744.037
AACGACC207.8581774E-444.031
ACCGTTA551.8189894E-1244.016
CGATATC351.4469515E-744.010
ATCGCCT408.3182385E-944.012
CCCATCG254.4442764E-544.036
AATCGCC408.3182385E-944.011
TACGTAG800.044.01
CCCGAGT207.8581774E-444.027
Produced by FastQC (version 0.11.5)