FastQCFastQC Report
Thu 26 May 2016
SRR1545836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545836_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2578860
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA209690.8131112196862179No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162380.6296580659671327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35610.13808426979362975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG650.044.0000041
TTAATCG302.5289464E-644.00000420
CAAGTCG302.5289464E-644.0000041
CACGGAC302.5289464E-644.00000432
ACGTTCC207.858305E-444.00000434
CTATCGC302.5289464E-644.00000432
TAGCGAT600.044.00000418
GCTATCG302.5289464E-644.00000412
ATTACAC207.858305E-444.00000417
TGTATCG650.044.00000428
AAGCGTC207.858305E-444.00000421
GAATACG302.5289464E-644.0000041
CGACAAC408.3200575E-944.00000418
TATGCGT302.5289464E-644.00000420
GCGCATC302.5289464E-644.00000417
TTATCGG1450.044.0000042
TCGATAT302.5289464E-644.00000416
CCGTCGA207.858305E-444.00000427
AATGCGC302.5289464E-644.00000414
TCAATCG408.3200575E-944.00000418
Produced by FastQC (version 0.11.5)