##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545836_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2578860
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.749783625322817	31.0	31.0	33.0	30.0	34.0
2	32.16713470293075	33.0	31.0	34.0	30.0	34.0
3	32.16332100230334	34.0	31.0	34.0	30.0	34.0
4	35.83719821936825	37.0	35.0	37.0	35.0	37.0
5	35.81914954669893	37.0	35.0	37.0	35.0	37.0
6	35.86606329928728	37.0	35.0	37.0	35.0	37.0
7	36.123037311059925	37.0	35.0	37.0	35.0	37.0
8	36.0923908238524	37.0	35.0	37.0	35.0	37.0
9	37.90892991476854	39.0	39.0	39.0	35.0	39.0
10	37.649350100431974	39.0	37.0	39.0	35.0	39.0
11	37.288667860992845	39.0	37.0	39.0	35.0	39.0
12	35.88853291764578	37.0	35.0	39.0	33.0	39.0
13	35.45414330363029	35.0	35.0	39.0	32.0	39.0
14	36.22224161063416	36.0	35.0	41.0	32.0	41.0
15	36.5548261634986	36.0	35.0	41.0	33.0	41.0
16	36.7436960517438	36.0	35.0	41.0	33.0	41.0
17	36.70506813087954	36.0	35.0	41.0	33.0	41.0
18	36.66141667248319	36.0	35.0	40.0	33.0	41.0
19	36.580279270685494	36.0	35.0	40.0	32.0	41.0
20	36.34159473565839	36.0	35.0	40.0	32.0	41.0
21	36.18795630627486	35.0	35.0	40.0	32.0	41.0
22	36.160966473558084	35.0	35.0	40.0	32.0	41.0
23	36.19577526503959	35.0	35.0	40.0	32.0	41.0
24	36.158411468633425	35.0	35.0	40.0	32.0	41.0
25	36.06942214777072	35.0	35.0	40.0	32.0	41.0
26	35.94209960990515	35.0	35.0	40.0	32.0	41.0
27	35.829117129274174	35.0	35.0	40.0	32.0	41.0
28	35.872129933381416	36.0	35.0	40.0	32.0	41.0
29	35.90540471371071	36.0	35.0	40.0	32.0	41.0
30	35.82525185547102	36.0	35.0	40.0	32.0	41.0
31	35.57916792691344	35.0	35.0	40.0	31.0	41.0
32	35.3345059444871	35.0	35.0	40.0	31.0	41.0
33	35.16084936755	35.0	35.0	40.0	30.0	41.0
34	34.9664103518609	35.0	35.0	40.0	30.0	41.0
35	34.7175732688087	35.0	34.0	40.0	29.0	41.0
36	34.55430655405877	35.0	34.0	40.0	27.0	41.0
37	34.458265280007446	35.0	34.0	40.0	27.0	41.0
38	34.43235499406715	35.0	34.0	40.0	27.0	41.0
39	34.45751223408793	35.0	34.0	40.0	27.0	41.0
40	34.247997177047225	35.0	34.0	40.0	25.0	41.0
41	34.24809683348456	35.0	34.0	40.0	25.0	41.0
42	34.230543728624276	35.0	34.0	40.0	25.0	41.0
43	34.13009120308974	35.0	34.0	40.0	25.0	41.0
44	33.98719240284467	35.0	34.0	40.0	24.0	41.0
45	33.85283536136122	35.0	34.0	39.0	23.0	41.0
46	33.831059848149955	35.0	34.0	39.0	23.0	41.0
47	33.789247962277905	35.0	34.0	39.0	23.0	41.0
48	33.77414904260022	35.0	34.0	39.0	23.0	41.0
49	33.7880218391072	35.0	34.0	39.0	24.0	41.0
50	33.534116625175464	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	2.0
13	4.0
14	16.0
15	36.0
16	109.0
17	328.0
18	780.0
19	1744.0
20	3225.0
21	5315.0
22	8243.0
23	12671.0
24	18706.0
25	28274.0
26	40334.0
27	49741.0
28	51714.0
29	53342.0
30	59987.0
31	71373.0
32	90724.0
33	123663.0
34	268352.0
35	667416.0
36	130956.0
37	141506.0
38	226460.0
39	523832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.070069720729315	24.057684403185903	30.776544674778776	15.095701201306003
2	30.57405210054055	26.31899366386698	29.761600086860085	13.345354148732385
3	25.524495319637357	24.522036869004133	34.99329160947085	14.960176201887657
4	24.285226805642804	26.808473511551618	33.81040459738024	15.095895085425342
5	22.25657848816919	31.852136215226885	32.47314704947147	13.418138247132456
6	20.572772465352905	37.83326741273276	29.758110172711973	11.83584994920236
7	84.79413384208526	5.0851926820378	8.10699301241634	2.0136804634605987
8	86.77737449880955	3.6835268296844346	7.531932714455225	2.00716595705079
9	83.82657453293315	4.771100408707723	8.966364982976973	2.4359600753821455
10	62.519524130817494	17.92156224067999	11.936126815724778	7.622786812777739
11	58.874890455472574	14.68427910006747	17.796429430058243	8.644401014401712
12	50.577735898807994	19.19751363005359	20.70100742188409	9.523743049254321
13	19.157302063702566	50.37338203702411	21.198591625757118	9.270724273516205
14	11.255205788604266	51.46460063749099	28.211147561325546	9.069046012579202
15	9.335636676671086	20.061887810893186	61.150702248280254	9.451773264155479
16	10.61023087720931	14.23442916637584	57.17933505502432	17.97600490139054
17	11.189285188028819	13.945890819974718	31.47514793358306	43.38967605841341
18	20.148980557300515	20.325104891308563	39.66838060228163	19.857533949109296
19	30.935645983108817	21.26703271988398	28.062515995439846	19.73480530156736
20	31.90735441241479	20.811211155316688	26.352845831103664	20.928588601164854
21	19.816857060871857	28.274741552468917	30.68759064082579	21.22081074583343
22	21.188470874727592	24.787968327090265	25.441241478792954	28.58231931938919
23	15.161505471409848	31.95140488432873	25.77200778638623	27.115081857875182
24	17.39524441032084	21.402169951063648	46.8153757862001	14.387209852415408
25	12.433711019597807	23.10451129568879	43.537570864645616	20.92420682006778
26	12.677656018550831	37.48346168462034	30.19372901204408	19.64515328478475
27	14.98185244642982	38.27737837649194	30.03067246767952	16.710096709398726
28	11.25532211907587	29.2300086084549	43.892689017627944	15.621980254841286
29	11.182072698789387	24.388101719364368	43.56770045679099	20.862125125055258
30	16.13302001659648	32.38206029020575	33.64769704443049	17.837222648767284
31	30.837385511427527	25.14316403372033	27.055016557703794	16.96443389714835
32	32.783400417238624	25.620041413647893	28.375095972639073	13.221462196474413
33	30.382998689343353	26.039606647898683	25.90935529652637	17.668039366231593
34	18.881327408234647	25.49498615667388	31.043910875348026	24.579775559743453
35	18.860038931931165	24.506797577224045	34.82914931403799	21.804014176806806
36	34.41792109691879	21.664456387706196	27.868515545628686	16.049106969746322
37	18.328447453525975	32.72321878659562	32.2725157627789	16.675817997099493
38	19.40590028151974	34.792195000891866	23.928247365114817	21.873657352473575
39	19.56034837098563	32.95277758389366	28.97206517608556	18.514808869035154
40	23.728469168547342	24.105728887958243	25.376910727996094	26.788891215498325
41	14.925470944525877	22.624027671141512	28.35380749633559	34.09669388799702
42	20.821176799050743	23.858022537090033	25.74591098392313	29.574889679936096
43	22.261503144800415	24.177543565761617	27.541975911837014	26.018977377600955
44	17.54426374444522	32.49823565451401	29.440372877938316	20.51712772310246
45	16.10498437293998	41.74972662339173	21.035612635040287	21.109676368628
46	22.422000418789697	34.38829560348371	25.495296371264825	17.69440760646177
47	22.349875526395387	27.124194411484144	27.213807651442885	23.312122410677585
48	23.76135191518733	22.15955887485168	33.00578550212109	21.073303707839898
49	22.133694733331783	21.33144102432858	35.77026282931218	20.76460141302746
50	18.20257788325074	35.748198816531335	25.60042034077073	20.4488029594472
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4361.0
1	4596.0
2	4831.0
3	16393.0
4	27955.0
5	20883.0
6	13811.0
7	14597.0
8	15383.0
9	16527.5
10	17672.0
11	17203.0
12	16734.0
13	15825.5
14	14917.0
15	14140.5
16	13364.0
17	12206.5
18	11049.0
19	10081.5
20	9114.0
21	8231.0
22	7348.0
23	8474.5
24	9601.0
25	11340.0
26	13079.0
27	12914.5
28	12750.0
29	17417.0
30	22084.0
31	22433.5
32	22783.0
33	30961.0
34	39139.0
35	42420.0
36	45701.0
37	53732.5
38	61764.0
39	104194.5
40	146625.0
41	206032.0
42	265439.0
43	320245.0
44	375051.0
45	375431.5
46	375812.0
47	319640.0
48	263468.0
49	231258.0
50	199048.0
51	186283.0
52	173518.0
53	147595.5
54	121673.0
55	107082.0
56	92491.0
57	77696.0
58	62901.0
59	54077.5
60	45254.0
61	39437.0
62	33620.0
63	26496.5
64	19373.0
65	14157.0
66	8941.0
67	6950.0
68	4959.0
69	3793.0
70	2627.0
71	2364.5
72	2102.0
73	1662.0
74	1222.0
75	975.0
76	728.0
77	520.5
78	313.0
79	222.5
80	132.0
81	92.0
82	52.0
83	46.5
84	41.0
85	28.0
86	15.0
87	14.0
88	13.0
89	6.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2578860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.880280721427656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6254514521117	9.998376058307528
2	9.771136038780451	2.517099502935017
3	3.7373099770526648	1.4441280494229205
4	1.8855214596151932	0.9714418282447883
5	1.1191168892895655	0.720726984707024
6	0.7366021819380303	0.5692585730026775
7	0.5097955220387662	0.45964166040702475
8	0.3821824350294863	0.3938093639982857
9	0.2750963053627136	0.3188985874649402
>10	2.0751428585022365	5.411324057922443
>50	0.43928392963404583	4.079442879301705
>100	1.0476399977351445	35.199806078516694
>500	0.3743964981727608	32.891090111011586
>1k	0.020715184601910283	3.565033509394201
>5k	0.0	0.0
>10k+	6.092701353503024E-4	1.4599227553631535
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	20969	0.8131112196862179	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16238	0.6296580659671327	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3561	0.13808426979362975	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06518384092195777	0.0
2	0.0	0.0	0.0	0.28466066401433193	0.0
3	0.0	0.0	0.0	0.40804851756202354	0.0
4	0.0	0.0	0.0	0.6301233878535477	0.0
5	0.0	0.0	0.0	1.061709437503393	0.0
6	0.0	0.0	0.0	1.4414508736418417	0.0
7	0.0	0.0	0.0	1.7044740699378795	0.0
8	0.0	0.0	0.0	2.296518616753139	0.0
9	0.0	0.0	0.0	2.5580295169183285	0.0
10	0.0	0.0	0.0	3.012997991360524	0.0
11	0.0	0.0	0.0	3.377771573485959	0.0
12	0.0	0.0	0.0	3.7315325376329076	0.0
13	0.0	0.0	0.0	3.884080562729268	0.0
14	0.0	0.0	0.0	3.9268513994555736	0.0
15	0.0	0.0	0.0	3.9952537167585676	0.0
16	0.0	0.0	0.0	4.175488394096616	0.0
17	0.0	0.0	0.0	4.3868608610005975	0.0
18	0.0	0.0	0.0	4.778661889361966	0.0
19	0.0	0.0	0.0	4.898986373824092	0.0
20	0.0	0.0	0.0	5.037807403271213	0.0
21	0.0	0.0	0.0	5.196559720186439	0.0
22	0.0	0.0	0.0	5.34612192984497	0.0
23	0.0	0.0	0.0	5.532056800291602	0.0
24	0.0	0.0	0.0	5.6546691173619354	0.0
25	0.0	0.0	0.0	5.752619374452277	0.0
26	0.0	0.0	0.0	5.863870082129313	0.0
27	0.0	0.0	0.0	5.967365425032766	0.0
28	0.0	0.0	0.0	6.083463235693291	0.0
29	0.0	0.0	0.0	6.1981650806945705	0.0
30	0.0	0.0	0.0	6.366572826752907	0.0
31	0.0	0.0	0.0	6.537462289538788	0.0
32	0.0	0.0	0.0	6.678920143008926	0.0
33	0.0	0.0	0.0	6.811536880637181	0.0
34	0.0	0.0	0.0	6.951637545271942	0.0
35	0.0	0.0	0.0	7.150640205362059	0.0
36	0.0	0.0	0.0	7.301171835617288	0.0
37	0.0	0.0	0.0	7.445964495940067	0.0
38	0.0	0.0	0.0	7.583893658438225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	65	0.0	44.000004	1
TTAATCG	30	2.5289464E-6	44.000004	20
CAAGTCG	30	2.5289464E-6	44.000004	1
CACGGAC	30	2.5289464E-6	44.000004	32
ACGTTCC	20	7.858305E-4	44.000004	34
CTATCGC	30	2.5289464E-6	44.000004	32
TAGCGAT	60	0.0	44.000004	18
GCTATCG	30	2.5289464E-6	44.000004	12
ATTACAC	20	7.858305E-4	44.000004	17
TGTATCG	65	0.0	44.000004	28
AAGCGTC	20	7.858305E-4	44.000004	21
GAATACG	30	2.5289464E-6	44.000004	1
CGACAAC	40	8.3200575E-9	44.000004	18
TATGCGT	30	2.5289464E-6	44.000004	20
GCGCATC	30	2.5289464E-6	44.000004	17
TTATCGG	145	0.0	44.000004	2
TCGATAT	30	2.5289464E-6	44.000004	16
CCGTCGA	20	7.858305E-4	44.000004	27
AATGCGC	30	2.5289464E-6	44.000004	14
TCAATCG	40	8.3200575E-9	44.000004	18
>>END_MODULE