FastQCFastQC Report
Thu 26 May 2016
SRR1545834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545834_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2184139
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111150.5088961828894589No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA81740.3742435806512315No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA40100.18359637367401982No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA33230.15214233160069027No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA27330.1251293988157347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA551.8189894E-1244.00000443
CGACGTA207.858039E-444.00000442
CGCATAG207.858039E-444.00000414
CGACATA408.3182385E-944.00000440
ATATGCG1100.044.0000041
ATCACGC207.858039E-444.0000048
ATATCCG408.3182385E-944.00000429
AACATCG207.858039E-444.00000434
CTACGAT207.858039E-444.00000441
ACTACGG800.044.0000042
CACCGTT207.858039E-444.00000424
CAATACG750.044.0000041
TAATGCG551.8189894E-1244.0000041
GCCCGTT207.858039E-444.00000433
GCGTAAG950.044.0000041
AACGTAT302.5287682E-644.015
ACGTTAG850.044.01
TAGCCGA254.44416E-544.01
CGCTATA254.44416E-544.039
CGCTAAT351.4468787E-744.027
Produced by FastQC (version 0.11.5)