##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545834_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2184139
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.694471826197876	31.0	31.0	33.0	30.0	34.0
2	32.11829787389905	33.0	31.0	34.0	30.0	34.0
3	32.19555623520298	33.0	31.0	34.0	30.0	34.0
4	35.87588930924268	37.0	35.0	37.0	35.0	37.0
5	35.8542835414779	37.0	35.0	37.0	35.0	37.0
6	35.903591300736814	37.0	35.0	37.0	35.0	37.0
7	36.079422600850954	37.0	35.0	37.0	35.0	37.0
8	36.031505778707306	37.0	35.0	37.0	35.0	37.0
9	37.790808185742755	39.0	38.0	39.0	35.0	39.0
10	37.562208266049	39.0	37.0	39.0	35.0	39.0
11	37.29406141275807	39.0	37.0	39.0	34.0	39.0
12	36.41803520746619	38.0	35.0	39.0	33.0	39.0
13	36.114443265744534	38.0	35.0	39.0	33.0	39.0
14	37.077603119581674	39.0	35.0	41.0	33.0	41.0
15	37.29144482104848	39.0	35.0	41.0	33.0	41.0
16	37.39094398296079	39.0	35.0	41.0	33.0	41.0
17	37.316624079328285	39.0	35.0	41.0	33.0	41.0
18	37.27913470708595	39.0	35.0	41.0	33.0	41.0
19	37.2026501060601	38.0	35.0	41.0	33.0	41.0
20	37.02928293483153	38.0	35.0	41.0	33.0	41.0
21	36.90702697951	38.0	35.0	41.0	33.0	41.0
22	36.897178705201455	38.0	35.0	41.0	33.0	41.0
23	36.899937229269746	38.0	35.0	41.0	33.0	41.0
24	36.86017510790293	38.0	35.0	41.0	33.0	41.0
25	36.75314574759207	38.0	35.0	41.0	32.0	41.0
26	36.647911602695615	38.0	35.0	41.0	32.0	41.0
27	36.55942135550897	38.0	35.0	41.0	32.0	41.0
28	36.525075556088694	38.0	35.0	41.0	32.0	41.0
29	36.517917586747	38.0	35.0	41.0	32.0	41.0
30	36.43801516295437	37.0	35.0	40.0	32.0	41.0
31	36.279506020450164	37.0	35.0	40.0	31.0	41.0
32	36.10343251963359	37.0	35.0	41.0	31.0	41.0
33	35.95202182644969	37.0	35.0	41.0	30.0	41.0
34	35.81523382898249	37.0	35.0	40.0	30.0	41.0
35	35.63104774925039	37.0	35.0	40.0	30.0	41.0
36	35.47482646479917	37.0	35.0	40.0	29.0	41.0
37	35.36632604426733	37.0	35.0	40.0	29.0	41.0
38	35.300949252771915	37.0	34.0	40.0	29.0	41.0
39	35.28320999716593	37.0	35.0	40.0	28.0	41.0
40	35.13034655761378	36.0	34.0	40.0	27.0	41.0
41	35.05600559305063	36.0	34.0	40.0	27.0	41.0
42	35.03189540592426	36.0	34.0	40.0	27.0	41.0
43	34.96571921475694	36.0	34.0	40.0	27.0	41.0
44	34.80888121131485	36.0	34.0	40.0	27.0	41.0
45	34.734378627001306	36.0	34.0	40.0	26.0	41.0
46	34.704490419336864	35.0	34.0	40.0	26.0	41.0
47	34.64916930653223	35.0	34.0	40.0	26.0	41.0
48	34.59618137856611	35.0	34.0	40.0	26.0	41.0
49	34.53629874289136	36.0	34.0	40.0	26.0	41.0
50	34.34990721744358	35.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	7.0
12	10.0
13	10.0
14	38.0
15	57.0
16	136.0
17	295.0
18	724.0
19	1435.0
20	2586.0
21	4341.0
22	6552.0
23	9680.0
24	13905.0
25	20143.0
26	28721.0
27	36380.0
28	39290.0
29	41411.0
30	46384.0
31	55006.0
32	68569.0
33	92320.0
34	188126.0
35	396164.0
36	121741.0
37	155297.0
38	262613.0
39	592192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.677407436065195	23.597582388300378	29.288108494926373	14.43690168070805
2	31.40312956272472	26.029662031583157	29.292458034951075	13.274750370741057
3	30.02945325366197	23.845002538757836	32.75185324743526	13.373690960144934
4	26.516993652876486	26.585533246739335	31.742347900019187	15.155125200364994
5	22.991714355176114	32.37820486699793	30.130728859289636	14.499351918536322
6	21.31979695431472	40.786781427372524	27.49637271254256	10.397048905770191
7	85.62298461773725	5.197746114143834	7.235162230975227	1.9441070371436984
8	87.54571023181217	3.7024658229169485	7.11131480185098	1.6405091434199013
9	82.42176894419265	6.002823080399187	8.603756445903855	2.9716515295043036
10	51.806592895415534	23.92283641288398	14.203262704434106	10.067307987266378
11	46.6394309153401	19.654335186542614	21.205243805453776	12.500990092663516
12	42.420651799175786	20.51032466340283	24.22876932283156	12.840254214589821
13	21.28843448150507	39.82676926697431	24.415066989784076	14.469729261736546
14	15.059481104453518	41.13250118238811	29.327208570516806	14.480809142641563
15	15.21569826828787	22.471738291381637	48.21249929606128	14.100064144269206
16	16.873605571806557	17.953024052040647	46.09834813626789	19.075022239884916
17	16.471479150365433	18.92741258683628	30.40387997284056	34.197228289957735
18	21.723754761029404	23.040795480507423	34.88376884438216	20.35168091408102
19	29.1185680032269	23.83058037972858	27.14149603115919	19.90935558588533
20	30.611696416757354	22.77167341455832	26.122467480320623	20.494162688363698
21	22.611152495331112	27.048186951471497	28.85997640260075	21.480684150596645
22	22.102210527809817	25.116029703237754	26.743032380265173	26.03872738868726
23	19.632083855468903	29.162612819055926	26.84385013957445	24.361453185900714
24	19.528747941408493	22.962595329326568	40.02419259946368	17.484464129801264
25	17.01471380713407	25.427639907533354	36.36265823741071	21.19498804792186
26	16.72558385707137	33.089057060928816	28.578629839950665	21.606729242049155
27	18.216697746800914	32.979952283256694	30.1372760616426	18.666073908299794
28	15.14871535190755	27.998080708233314	39.271767959823066	17.58143598003607
29	16.490708695737773	24.425185393420474	37.32399815213226	21.760107758709495
30	18.765655482549416	30.02221928183142	31.899984387440544	19.31214084817862
31	28.418429413146324	27.09790906164855	25.913781128398877	18.569880396806244
32	29.494093553569623	26.002465960270843	27.132796951109796	17.370643535049737
33	26.51278146674731	28.381984846202553	26.029341539160285	19.075892147889856
34	18.9281451409457	27.607583583279272	30.111590883180973	23.35268039259406
35	20.682474879117127	25.25274261390873	31.977223061352777	22.087559445621363
36	28.763920245002723	25.84615722717281	26.17832473116409	19.211597796660378
37	20.551347693530495	33.11762667119629	28.344212524935454	17.986813110337756
38	20.479740529334443	33.354882633385515	24.239849203736576	21.92552763354347
39	21.46484266798038	31.769086125013107	26.955198364206673	19.81087284279984
40	23.770831435178806	27.020304110681597	25.399619712847947	23.80924474129165
41	17.457817474071017	25.97682656644106	26.789045935263278	29.77631002422465
42	21.577518646935932	26.698209225694885	24.622013525695937	27.102258601673245
43	22.442115634581867	26.269069871468805	25.596264706596056	25.692549787353276
44	19.026215822344643	30.968404483414286	28.459544012537663	21.54583568170341
45	17.885125443023544	36.81702492378003	23.240965890907127	22.056883742289298
46	21.200253280583333	33.13172833780268	26.154928784294405	19.513089597319585
47	22.05921875851308	28.09042831065239	27.3647418960057	22.48561103482883
48	22.757205470897226	24.53429017109259	31.224203221498264	21.484301136511917
49	20.984195602935525	25.467106260178497	31.831765286000568	21.716932850885406
50	19.33791759590392	32.79228107734902	27.643432950009135	20.226368376737927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1267.0
1	2973.0
2	4679.0
3	13813.5
4	22948.0
5	16813.5
6	10679.0
7	10412.0
8	10145.0
9	10022.0
10	9899.0
11	9657.0
12	9415.0
13	8923.0
14	8431.0
15	8195.5
16	7960.0
17	7038.0
18	6116.0
19	6138.5
20	6161.0
21	6518.5
22	6876.0
23	8075.0
24	9274.0
25	11350.5
26	13427.0
27	19323.0
28	25219.0
29	29861.0
30	34503.0
31	37647.5
32	40792.0
33	44282.0
34	47772.0
35	54914.0
36	62056.0
37	66342.5
38	70629.0
39	88101.5
40	105574.0
41	138079.0
42	170584.0
43	200396.0
44	230208.0
45	231310.0
46	232412.0
47	215208.5
48	198005.0
49	185641.0
50	173277.0
51	163729.5
52	154182.0
53	142070.5
54	129959.0
55	114703.5
56	99448.0
57	90612.5
58	81777.0
59	72737.5
60	63698.0
61	57502.5
62	51307.0
63	42991.5
64	34676.0
65	27925.0
66	21174.0
67	15870.0
68	10566.0
69	9167.5
70	7769.0
71	6107.5
72	4446.0
73	3640.5
74	2835.0
75	2364.5
76	1894.0
77	1550.0
78	1206.0
79	805.5
80	405.0
81	309.0
82	213.0
83	177.5
84	142.0
85	108.5
86	75.0
87	46.0
88	17.0
89	20.0
90	23.0
91	18.0
92	13.0
93	8.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2184139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.904196539313162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34610112732892	11.171479812419124
2	7.452882989762443	2.072526997483218
3	2.370021130580939	0.9885971880576766
4	1.1627777047056738	0.6466995895103652
5	0.6793744461415021	0.4723077911469235
6	0.48220630557995503	0.4022814747167878
7	0.3805420751907127	0.370379226345083
8	0.33583366380940183	0.37355978128988365
9	0.2695992873358799	0.33737053301791525
>10	3.400249736622317	11.479099017317782
>50	1.1206432047680073	11.181681658899146
>100	1.9721186911197495	56.70221666033761
>500	0.02431835548171283	2.087541714009981
>1k	0.002665025258269899	0.8208153434505063
>5k	3.331281572837374E-4	0.37860981983886
>10k+	3.331281572837374E-4	0.5148333921591545
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11115	0.5088961828894589	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	8174	0.3742435806512315	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4010	0.18359637367401982	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3323	0.15214233160069027	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2733	0.1251293988157347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019550037795213582	0.0
2	0.0	0.0	0.0	0.06881430165387825	0.0
3	0.0	0.0	0.0	0.09614772686170614	0.0
4	0.0	0.0	0.0	0.14806749936702746	0.0
5	0.0	0.0	0.0	0.26349055623291373	0.0
6	0.0	0.0	0.0	0.38101970616338976	0.0
7	0.0	0.0	0.0	0.47226847741833283	0.0
8	0.0	0.0	0.0	0.7215200131493462	0.0
9	0.0	0.0	0.0	0.8249932811052776	0.0
10	0.0	0.0	0.0	0.9854226310688102	0.0
11	0.0	0.0	0.0	1.1018529498351524	0.0
12	0.0	0.0	0.0	1.2261124406459478	0.0
13	0.0	0.0	0.0	1.2690126406790043	0.0
14	0.0	0.0	0.0	1.2829769533898712	0.0
15	0.0	0.0	0.0	1.30733437752817	0.0
16	0.0	0.0	0.0	1.36140602772992	0.0
17	4.5784631838907686E-5	0.0	0.0	1.4304950371748317	0.0
18	4.5784631838907686E-5	0.0	0.0	1.5461928018317515	0.0
19	4.5784631838907686E-5	0.0	0.0	1.588131524596191	0.0
20	4.5784631838907686E-5	0.0	0.0	1.635701757076816	0.0
21	4.5784631838907686E-5	0.0	0.0	1.6987471951189919	0.0
22	4.5784631838907686E-5	0.0	0.0	1.7695302359419434	0.0
23	4.5784631838907686E-5	0.0	0.0	1.8513473730380714	0.0
24	4.5784631838907686E-5	0.0	0.0	1.9079371779909613	0.0
25	4.5784631838907686E-5	0.0	0.0	1.9593075349142157	0.0
26	4.5784631838907686E-5	0.0	0.0	2.0094417067778196	0.0
27	4.5784631838907686E-5	0.0	0.0	2.061773540969691	0.0
28	4.5784631838907686E-5	0.0	0.0	2.1164403913853467	0.0
29	4.5784631838907686E-5	0.0	0.0	2.175227858666504	0.0
30	4.5784631838907686E-5	0.0	0.0	2.252695455737936	0.0
31	4.5784631838907686E-5	0.0	0.0	2.328514806063167	0.0
32	4.5784631838907686E-5	0.0	0.0	2.4011292321596747	0.0
33	4.5784631838907686E-5	0.0	0.0	2.472827965619404	0.0
34	4.5784631838907686E-5	0.0	0.0	2.545717099506945	0.0
35	4.5784631838907686E-5	0.0	0.0	2.643101011428302	0.0
36	4.5784631838907686E-5	0.0	0.0	2.7300918119222266	0.0
37	4.5784631838907686E-5	0.0	0.0	2.8136029803963942	0.0
38	4.5784631838907686E-5	0.0	0.0	2.897526210557112	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	55	1.8189894E-12	44.000004	43
CGACGTA	20	7.858039E-4	44.000004	42
CGCATAG	20	7.858039E-4	44.000004	14
CGACATA	40	8.3182385E-9	44.000004	40
ATATGCG	110	0.0	44.000004	1
ATCACGC	20	7.858039E-4	44.000004	8
ATATCCG	40	8.3182385E-9	44.000004	29
AACATCG	20	7.858039E-4	44.000004	34
CTACGAT	20	7.858039E-4	44.000004	41
ACTACGG	80	0.0	44.000004	2
CACCGTT	20	7.858039E-4	44.000004	24
CAATACG	75	0.0	44.000004	1
TAATGCG	55	1.8189894E-12	44.000004	1
GCCCGTT	20	7.858039E-4	44.000004	33
GCGTAAG	95	0.0	44.000004	1
AACGTAT	30	2.5287682E-6	44.0	15
ACGTTAG	85	0.0	44.0	1
TAGCCGA	25	4.44416E-5	44.0	1
CGCTATA	25	4.44416E-5	44.0	39
CGCTAAT	35	1.4468787E-7	44.0	27
>>END_MODULE