FastQCFastQC Report
Thu 26 May 2016
SRR1545833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545833_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3869736
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227470.587817876981789No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA132090.3413411147427111No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA84900.2193948114289967No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA62990.162775961977768No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA53160.13737371231525872No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA44730.11558928050905799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.858793E-444.0000041
ACTCTCG207.858793E-444.00000426
CTACGAT207.858793E-444.00000423
TACGTCC408.3218765E-944.00000435
CGAACGT351.4472789E-744.038
CGAACGA254.444801E-544.022
GTCGAAC302.5292775E-644.021
GGTCGAA551.8189894E-1244.08
TATGCGC502.7284841E-1144.020
AATGCGC600.044.033
TCGAACA502.7284841E-1144.037
GTTATGC302.5292775E-644.020
TAATGCG1050.043.9999961
CGCTTAC454.802132E-1043.99999618
TTATACG1350.042.370371
TAGTGCG2850.041.6842081
TCGAATA850.041.41176619
ACATACG12250.041.3061217
GCTACGA6300.041.2063510
CATACGA12250.041.1265318
Produced by FastQC (version 0.11.5)