##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545833_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3869736
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74289874037919	31.0	31.0	33.0	30.0	34.0
2	32.17390230237928	33.0	31.0	34.0	30.0	34.0
3	32.23571633827217	34.0	31.0	34.0	30.0	34.0
4	35.91432102861797	37.0	35.0	37.0	35.0	37.0
5	35.90214345371364	37.0	35.0	37.0	35.0	37.0
6	35.956106308027216	37.0	35.0	37.0	35.0	37.0
7	36.119531151479066	37.0	35.0	37.0	35.0	37.0
8	36.05509238873143	37.0	35.0	37.0	35.0	37.0
9	37.80611338861359	39.0	38.0	39.0	35.0	39.0
10	37.59322212161243	39.0	37.0	39.0	35.0	39.0
11	37.352558675837315	39.0	37.0	39.0	35.0	39.0
12	36.56295028911533	38.0	35.0	39.0	33.0	39.0
13	36.3154504596696	38.0	35.0	39.0	33.0	39.0
14	37.325851427590926	39.0	35.0	41.0	33.0	41.0
15	37.492944996764635	39.0	35.0	41.0	33.0	41.0
16	37.56891503709814	39.0	35.0	41.0	33.0	41.0
17	37.50988465363012	39.0	35.0	41.0	33.0	41.0
18	37.45726168400118	39.0	35.0	41.0	33.0	41.0
19	37.37679211191668	39.0	35.0	41.0	33.0	41.0
20	37.2074924490973	38.0	35.0	41.0	33.0	41.0
21	37.101053922024654	38.0	35.0	41.0	33.0	41.0
22	37.091132831800415	38.0	35.0	41.0	33.0	41.0
23	37.09162046196433	38.0	35.0	41.0	33.0	41.0
24	37.060162502041486	38.0	35.0	41.0	33.0	41.0
25	36.96541185238476	38.0	35.0	41.0	33.0	41.0
26	36.87115348437206	38.0	35.0	41.0	33.0	41.0
27	36.7666114691028	38.0	35.0	41.0	32.0	41.0
28	36.7597696587054	38.0	35.0	41.0	32.0	41.0
29	36.73259700403335	38.0	35.0	41.0	32.0	41.0
30	36.64826463614055	38.0	35.0	41.0	32.0	41.0
31	36.49524618733681	38.0	35.0	41.0	32.0	41.0
32	36.32169429645847	38.0	35.0	41.0	31.0	41.0
33	36.16585756754466	38.0	35.0	41.0	31.0	41.0
34	36.00119982345049	38.0	35.0	41.0	30.0	41.0
35	35.807134646911315	38.0	35.0	41.0	30.0	41.0
36	35.64493650212831	38.0	35.0	40.0	29.0	41.0
37	35.568870072790496	38.0	35.0	40.0	29.0	41.0
38	35.505930895544296	38.0	35.0	40.0	29.0	41.0
39	35.479381280790214	37.0	35.0	40.0	29.0	41.0
40	35.30031583549886	37.0	35.0	40.0	27.0	41.0
41	35.234914474785874	37.0	34.0	40.0	28.0	41.0
42	35.22683795483723	37.0	35.0	40.0	27.0	41.0
43	35.16380393908008	37.0	35.0	40.0	27.0	41.0
44	35.051471986719505	37.0	34.0	40.0	27.0	41.0
45	34.944954642900704	36.0	34.0	40.0	27.0	41.0
46	34.916661498355445	36.0	34.0	40.0	27.0	41.0
47	34.876071907747715	36.0	34.0	40.0	27.0	41.0
48	34.808507608787785	36.0	34.0	40.0	27.0	41.0
49	34.746497952315096	36.0	34.0	40.0	26.0	41.0
50	34.585845132587856	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	12.0
12	10.0
13	23.0
14	40.0
15	95.0
16	196.0
17	471.0
18	1181.0
19	2467.0
20	4248.0
21	6714.0
22	10675.0
23	15146.0
24	22454.0
25	33808.0
26	49505.0
27	61910.0
28	66762.0
29	69715.0
30	78134.0
31	92180.0
32	115988.0
33	156222.0
34	315907.0
35	663463.0
36	217308.0
37	279348.0
38	476734.0
39	1129014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.79217600373772	23.98822555337108	28.371341094069468	13.848257348821729
2	31.86527453035556	26.157210724452522	28.863416005639664	13.11409873955226
3	29.959511449876686	24.029727092494166	32.87374125780156	13.137020199827584
4	26.21866194489753	27.437323889795067	31.597090860978632	14.74692330432877
5	23.01012265436195	32.47017367593035	30.13360601343347	14.386097656274227
6	20.9255876886692	40.92501400612342	27.69095876307841	10.458439542128971
7	85.27610152217102	5.180379230004321	7.647963582011796	1.8955556658128616
8	86.38062131370202	4.1878309011260715	7.489012170339268	1.9425356148326398
9	81.6509446639254	6.103207040480281	9.032993465187289	3.2128548304070352
10	48.91023573701151	25.345734179282513	14.956291591984568	10.787738491721399
11	44.04269438535342	20.754335696285224	21.914854139920656	13.288115778440702
12	39.81974480946504	21.975168331896544	25.35532139660173	12.849765462036686
13	22.040728359764078	38.16464482331611	25.096311479646154	14.698315337273653
14	15.911343822937793	39.251282258014506	28.98856666191182	15.848807257135888
15	15.704585532449759	23.098862558065978	46.71318663598757	14.483365273496693
16	17.107988762024075	19.286974615322595	44.3571861232911	19.24785049936223
17	17.68862268640548	20.019789463674005	29.77575214433233	32.51583570558819
18	21.81035088698557	23.467208098950422	34.957268402805774	19.765172611258237
19	28.835972273043947	23.67458658678525	26.963208859725828	20.52623228044497
20	29.094646249770012	23.215718074824743	27.17490288743211	20.514732787973134
21	21.974031303427417	27.834637815086094	29.598582435597677	20.592748445888816
22	22.29717996266412	24.602686074708974	27.363442880858024	25.736691081768885
23	19.525983167844007	29.350710229328303	27.427814197144194	23.69549240568349
24	19.303797468354432	23.530752485440868	39.45796302383419	17.70748702237052
25	17.96218139945464	26.020095427698426	35.078439459436	20.93928371341094
26	17.550111945621097	32.51480204334353	28.925745838992633	21.009340172042744
27	17.906260272018557	32.84720197966993	29.944239090211838	19.302298658099673
28	15.36006590630472	28.29916562783611	37.475088739903704	18.865679725955466
29	16.68762416867714	25.210298583675993	36.24223978069821	21.85983746694865
30	18.20372242447547	30.47440445549774	32.25023619182291	19.07163692820389
31	27.68183669376929	26.908941591881202	26.645977916839804	18.763243797509702
32	28.02428899542501	26.0750604175582	28.405710363704394	17.49494022331239
33	26.777614803697205	27.514486776358904	26.487336603840678	19.220561816103217
34	19.77550923370483	27.232865497801402	29.45733248986494	23.534292778628828
35	21.352231780152444	26.00577920560989	31.366454972638962	21.275534041598704
36	28.38441692146441	27.31700560451669	25.9946673364798	18.3039101375391
37	20.123336579032784	33.84031882278274	27.864743227961803	18.17160137022267
38	20.263036031398524	33.341318374173326	25.35834485866736	21.037300735760788
39	20.917964429614837	31.65864544764811	27.660181469743673	19.763208652993384
40	23.698567550861352	26.71714556238462	26.512997269064346	23.071289617689683
41	16.90670888143274	26.726086740800923	28.09276911913371	28.27443525863263
42	21.344841095103128	26.13142085144826	26.135322926421857	26.388415127026754
43	21.773888451305208	26.98016092053825	26.751540673575665	24.49440995458088
44	19.088072157893976	30.920688129629514	28.175410415594243	21.81582929688227
45	17.43736523628485	36.88711581358521	23.807954857902452	21.86756409222748
46	20.33322169781091	34.56432170049843	26.500722529909016	18.601734071781642
47	22.010131957322155	27.95965926357767	27.826446041797166	22.20376273730301
48	22.34341050655652	25.322037472323693	31.647275162956852	20.687276858162935
49	20.512226157029833	25.85920589931716	32.61139778010696	21.01717016354604
50	19.13412181089356	32.910255376594165	27.973122714314364	19.982500098197914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2166.0
1	5978.0
2	9790.0
3	27504.5
4	45219.0
5	32617.0
6	20015.0
7	19745.5
8	19476.0
9	19163.0
10	18850.0
11	18160.0
12	17470.0
13	16119.0
14	14768.0
15	14089.5
16	13411.0
17	12293.0
18	11175.0
19	11897.5
20	12620.0
21	12659.5
22	12699.0
23	13279.5
24	13860.0
25	22079.5
26	30299.0
27	38323.5
28	46348.0
29	57355.0
30	68362.0
31	76073.0
32	83784.0
33	89543.0
34	95302.0
35	104767.0
36	114232.0
37	120609.5
38	126987.0
39	161434.5
40	195882.0
41	255950.5
42	316019.0
43	361321.0
44	406623.0
45	405467.0
46	404311.0
47	365268.5
48	326226.0
49	307302.5
50	288379.0
51	273476.0
52	258573.0
53	242258.0
54	225943.0
55	205896.5
56	185850.0
57	163107.5
58	140365.0
59	133843.0
60	127321.0
61	108348.0
62	89375.0
63	71213.0
64	53051.0
65	42132.5
66	31214.0
67	23996.0
68	16778.0
69	13830.0
70	10882.0
71	8738.0
72	6594.0
73	5222.5
74	3851.0
75	3352.0
76	2853.0
77	2167.0
78	1481.0
79	1121.0
80	761.0
81	529.0
82	297.0
83	212.5
84	128.0
85	99.0
86	70.0
87	54.5
88	39.0
89	26.0
90	13.0
91	12.0
92	11.0
93	9.5
94	8.0
95	6.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3869736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.27103381196416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96043439479237	9.811971940778392
2	8.632816226148837	2.118671596070904
3	2.8482121709941275	1.0485152356175036
4	1.3968179697829564	0.6856160214546316
5	0.7564887755580549	0.464144967162213
6	0.5405236888091445	0.3979670676926763
7	0.3802090409590713	0.32658905980562614
8	0.27677249069705756	0.2717027673252103
9	0.2171041545447897	0.2397683179023306
>10	2.3180633954621204	6.5917911655661605
>50	0.7921363012898334	7.009027573872998
>100	1.6297553814428902	50.08750982569927
>500	0.23830323013772445	17.571936337662304
>1k	0.01129702253783488	1.9084635300104937
>5k	6.394541059151818E-4	0.5258638973598434
>10k+	4.263027372767879E-4	0.9404606960194245
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22747	0.587817876981789	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	13209	0.3413411147427111	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8490	0.2193948114289967	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6299	0.162775961977768	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	5316	0.13737371231525872	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4473	0.11558928050905799	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028218979279206642	0.0
2	0.0	0.0	0.0	0.11269502622401115	0.0
3	0.0	0.0	0.0	0.15688408718320837	0.0
4	0.0	0.0	0.0	0.22267668905579088	0.0
5	0.0	0.0	0.0	0.3791214697850189	0.0
6	0.0	0.0	0.0	0.5064428167709631	0.0
7	0.0	0.0	0.0	0.6043564728963423	0.0
8	0.0	0.0	0.0	0.8262579152686385	0.0
9	0.0	0.0	0.0	0.9200369224153793	0.0
10	0.0	0.0	0.0	1.105811869336823	0.0
11	0.0	0.0	0.0	1.2519717107316881	0.0
12	0.0	0.0	0.0	1.4018010530950948	0.0
13	0.0	0.0	0.0	1.4560941624958395	0.0
14	0.0	0.0	0.0	1.4737439453234018	0.0
15	0.0	0.0	0.0	1.5093019265396916	0.0
16	0.0	0.0	0.0	1.5867749117769274	0.0
17	0.0	0.0	0.0	1.6817684720611432	0.0
18	0.0	0.0	0.0	1.830383261287075	0.0
19	0.0	0.0	0.0	1.8854516173713143	0.0
20	0.0	0.0	0.0	1.9480657078415686	0.0
21	0.0	0.0	0.0	2.031637300322296	0.0
22	0.0	0.0	0.0	2.1180256224197205	0.0
23	0.0	0.0	0.0	2.22071996642665	0.0
24	0.0	0.0	0.0	2.298012060771071	0.0
25	0.0	0.0	0.0	2.3638563457558863	0.0
26	0.0	0.0	0.0	2.4299848878579833	0.0
27	5.16831122329792E-5	0.0	0.0	2.4947696690420225	0.0
28	5.16831122329792E-5	0.0	0.0	2.5620610811693614	0.0
29	5.16831122329792E-5	0.0	0.0	2.633538825387572	0.0
30	5.16831122329792E-5	0.0	0.0	2.7260515962846044	0.0
31	5.16831122329792E-5	0.0	0.0	2.822027135701247	0.0
32	5.16831122329792E-5	0.0	0.0	2.9063481333093524	0.0
33	5.16831122329792E-5	0.0	0.0	2.9874389364028966	0.0
34	5.16831122329792E-5	0.0	0.0	3.0745766636277	0.0
35	5.16831122329792E-5	0.0	0.0	3.189907528575593	0.0
36	5.16831122329792E-5	0.0	0.0	3.2879503924815543	0.0
37	5.16831122329792E-5	0.0	0.0	3.3856573161580017	0.0
38	5.16831122329792E-5	0.0	0.0	3.480366619324936	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.858793E-4	44.000004	1
ACTCTCG	20	7.858793E-4	44.000004	26
CTACGAT	20	7.858793E-4	44.000004	23
TACGTCC	40	8.3218765E-9	44.000004	35
CGAACGT	35	1.4472789E-7	44.0	38
CGAACGA	25	4.444801E-5	44.0	22
GTCGAAC	30	2.5292775E-6	44.0	21
GGTCGAA	55	1.8189894E-12	44.0	8
TATGCGC	50	2.7284841E-11	44.0	20
AATGCGC	60	0.0	44.0	33
TCGAACA	50	2.7284841E-11	44.0	37
GTTATGC	30	2.5292775E-6	44.0	20
TAATGCG	105	0.0	43.999996	1
CGCTTAC	45	4.802132E-10	43.999996	18
TTATACG	135	0.0	42.37037	1
TAGTGCG	285	0.0	41.684208	1
TCGAATA	85	0.0	41.411766	19
ACATACG	1225	0.0	41.30612	17
GCTACGA	630	0.0	41.20635	10
CATACGA	1225	0.0	41.12653	18
>>END_MODULE