FastQCFastQC Report
Thu 26 May 2016
SRR1545832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545832_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3274978
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200210.6113323509348765No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA96200.2937424312468664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC207.8586163E-444.00000422
CGAATCC207.8586163E-444.00000422
CGACTAA408.3200575E-944.00000436
CCCGAAC408.3200575E-944.00000411
ACGTTGA254.4446493E-544.042
CGACATA351.447188E-744.028
TAGCACG502.7284841E-1144.01
CGTAGCA302.5291574E-644.028
TATTACG1200.044.01
CCGAATA351.447188E-744.019
TATACGC502.7284841E-1144.015
TAATGCG2050.044.01
CGTTTTT124750.041.6721461
AGGGCGA18750.041.301336
CGGTCTA3250.040.61538331
GGGCGAT39350.040.3659487
CGTAAGG4700.040.255322
CGACGGT3450.040.17391228
CCGTCGA557.8216544E-1140.018
GCGTAAG2750.040.01
Produced by FastQC (version 0.11.5)