FastQCFastQC Report
Thu 26 May 2016
SRR1545831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545831_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences169125
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.4523281596452328No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4570.2702143385070214No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA3040.17974870657797487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTTA207.8374264E-444.017
TTCTACG207.8374264E-444.01
CAAACGC207.8374264E-444.034
CGACGGT207.8374264E-444.028
ATAGCGG302.5148802E-644.02
CGACCCC207.8374264E-444.020
AGGGTAC351.4363104E-744.06
TGAGTAG302.5148802E-644.01
CACGACG207.8374264E-444.026
TGCGGGT207.8374264E-444.04
GGCTTAA254.4267006E-544.08
ACCTAGG207.8374264E-444.02
CGAGGGC351.4363104E-744.04
ACCAGAA454.7475623E-1044.02
GACGGTC207.8374264E-444.029
CGGGACT207.8374264E-444.06
GCGATAA254.4267006E-544.09
TATAGCG207.8374264E-444.01
CGTAAAT207.8374264E-444.016
CGGTCTA207.8374264E-444.031
Produced by FastQC (version 0.11.5)