##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545831_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	169125
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68062084257206	31.0	31.0	33.0	30.0	34.0
2	32.10570288248337	33.0	31.0	34.0	30.0	34.0
3	32.176946045824096	33.0	31.0	34.0	30.0	34.0
4	35.871071692535104	37.0	35.0	37.0	35.0	37.0
5	35.84269031781227	37.0	35.0	37.0	35.0	37.0
6	35.888768662232074	37.0	35.0	37.0	35.0	37.0
7	36.07969253510717	37.0	35.0	37.0	35.0	37.0
8	36.02877753141168	37.0	35.0	37.0	35.0	37.0
9	37.78287657058389	39.0	38.0	39.0	35.0	39.0
10	37.54615816703622	39.0	37.0	39.0	35.0	39.0
11	37.25772062084257	39.0	37.0	39.0	34.0	39.0
12	36.394039911308205	38.0	35.0	39.0	33.0	39.0
13	36.116541019955655	38.0	35.0	39.0	33.0	39.0
14	37.048656319290465	39.0	35.0	41.0	33.0	41.0
15	37.27569549150037	39.0	35.0	41.0	33.0	41.0
16	37.38169401330377	39.0	35.0	41.0	33.0	41.0
17	37.332747967479676	39.0	35.0	41.0	33.0	41.0
18	37.26141611234294	38.0	35.0	41.0	33.0	41.0
19	37.1880206947524	38.0	35.0	41.0	33.0	41.0
20	37.02368662232077	38.0	35.0	41.0	33.0	41.0
21	36.906134515890614	38.0	35.0	41.0	33.0	41.0
22	36.89759645232816	38.0	35.0	41.0	32.0	41.0
23	36.88966740576497	38.0	35.0	41.0	33.0	41.0
24	36.8570051736881	38.0	35.0	41.0	33.0	41.0
25	36.74115003695491	38.0	35.0	41.0	32.0	41.0
26	36.64176201034738	38.0	35.0	41.0	32.0	41.0
27	36.54277014042868	38.0	35.0	41.0	32.0	41.0
28	36.53163045084996	38.0	35.0	41.0	32.0	41.0
29	36.49579896526238	38.0	35.0	41.0	32.0	41.0
30	36.42708647450111	38.0	35.0	41.0	31.0	41.0
31	36.25439172209904	38.0	35.0	40.0	31.0	41.0
32	36.089614190687364	38.0	35.0	41.0	31.0	41.0
33	35.9209756097561	38.0	35.0	40.0	30.0	41.0
34	35.75761714708056	37.0	35.0	40.0	30.0	41.0
35	35.55168070953437	37.0	35.0	40.0	29.0	41.0
36	35.41937620103474	37.0	35.0	40.0	29.0	41.0
37	35.3319911308204	37.0	35.0	40.0	29.0	41.0
38	35.240023651145606	37.0	34.0	40.0	28.0	41.0
39	35.20238876570584	37.0	34.0	40.0	27.0	41.0
40	35.019725055432374	36.0	34.0	40.0	27.0	41.0
41	34.95343680709534	36.0	34.0	40.0	26.0	41.0
42	34.95995269770879	36.0	34.0	40.0	27.0	41.0
43	34.895432372505546	36.0	34.0	40.0	27.0	41.0
44	34.78930376940133	36.0	34.0	40.0	26.0	41.0
45	34.69073170731707	36.0	34.0	40.0	26.0	41.0
46	34.65311456023651	35.0	34.0	40.0	26.0	41.0
47	34.59354323725056	35.0	34.0	40.0	26.0	41.0
48	34.507429416112345	35.0	34.0	40.0	26.0	41.0
49	34.44760975609756	36.0	34.0	40.0	26.0	41.0
50	34.29517812269032	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	0.0
15	4.0
16	19.0
17	27.0
18	61.0
19	131.0
20	226.0
21	330.0
22	507.0
23	749.0
24	1082.0
25	1667.0
26	2307.0
27	2812.0
28	3139.0
29	3151.0
30	3632.0
31	4290.0
32	5383.0
33	7189.0
34	14635.0
35	29951.0
36	9718.0
37	12418.0
38	20585.0
39	45106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.20990391722099	24.493126385809312	29.858980044345895	13.4379896526238
2	31.679526977087953	25.41552106430155	29.7490022172949	13.155949741315595
3	28.8549889135255	24.5439763488544	33.420842572062085	13.180192165558019
4	27.095343680709533	26.56082779009608	31.66060606060606	14.683222468588323
5	22.598078344419807	32.51441241685144	30.513525498891354	14.373983739837398
6	21.09090909090909	41.20739098300074	27.44242424242424	10.259275683665928
7	86.47745750184774	4.490169992609017	7.000147819660015	2.0322246858832225
8	87.82143385070215	3.493865484109387	6.8753880266075384	1.8093126385809313
9	83.43532889874353	5.0838137472283815	8.458832224685883	3.0220251293422025
10	52.52564671101256	23.963340724316335	13.628972653362897	9.882039911308203
11	46.26991869918699	19.253215077605322	21.75609756097561	12.720768662232077
12	41.2339985218034	21.42439024390244	23.99940872135994	13.34220251293422
13	22.724611973392463	37.86962305986697	23.94560236511456	15.460162601626015
14	14.799113082039911	40.137176644493714	29.189061345158905	15.874648928307463
15	15.037989652623798	23.010199556541018	47.64464153732446	14.307169253510718
16	17.595861049519588	17.909830007390983	45.369992609017004	19.12431633407243
17	17.728898743532888	18.44079822616408	29.64671101256467	34.183592017738356
18	22.08189209164819	23.028529194382852	33.620694752402066	21.26888396156689
19	30.165853658536584	23.51160384331116	26.35210643015521	19.970436067997042
20	30.491056910569103	23.46430155210643	25.614190687361422	20.430450849963044
21	22.906134515890614	26.82690317812269	28.790539541759053	21.476422764227642
22	22.656023651145603	24.015964523281596	27.107760532150778	26.220251293422024
23	20.01241685144124	29.096230598669624	27.321212121212124	23.570140428677014
24	19.885291943828527	23.716186252771617	38.08662232076866	18.31189948263119
25	17.889726533628973	25.551515151515154	35.4849963045085	21.073762010347377
26	17.267110125646713	32.71840354767184	28.17087952697709	21.84360679970436
27	18.593939393939394	31.376201034737623	30.41359940872136	19.616260162601627
28	15.303473762010347	27.929046563192905	38.002069475240205	18.76541019955654
29	16.61670362158167	24.4629711751663	37.00931263858093	21.9110125646711
30	19.651145602365112	29.416703621581668	31.478492239467847	19.453658536585365
31	28.7739837398374	26.310125646711015	25.056023651145605	19.859866962305986
32	29.516629711751662	25.808130081300813	26.58920916481892	18.086031042128603
33	26.726385809312635	27.079379157427937	25.935846267553586	20.25838876570584
34	20.234146341463415	25.9529933481153	30.31249076127125	23.500369549150037
35	21.835920177383592	24.491943828529195	31.80192165558019	21.870214338507022
36	28.412712490761272	26.211973392461196	26.469179600886918	18.906134515890614
37	21.00339985218034	31.74988913525499	29.002217294900223	18.24449371766445
38	21.398965262379896	32.81655580192165	25.073170731707318	20.71130820399113
39	21.39778270509978	30.70983000739098	26.938654841093868	20.953732446415373
40	24.050258684405026	25.72594235033259	26.025720620842574	24.19807834441981
41	17.894456762749446	25.01640798226164	27.56836659275684	29.52076866223208
42	21.832963784183296	26.37753141167775	25.333333333333336	26.456171470805618
43	21.956541019955655	27.40339985218034	25.94530672579453	24.694752402069476
44	20.590687361419068	29.47228381374723	28.147228381374724	21.78980044345898
45	18.32431633407243	35.13555062823355	23.28277900960828	23.257354028085736
46	21.697560975609758	32.65572801182557	25.630155210643014	20.01655580192166
47	22.244493717664447	27.064597191426458	28.454101995565413	22.23680709534368
48	23.002512934220253	24.409756097560976	31.188174427198817	21.399556541019958
49	21.352845528455287	25.030007390983002	32.031337767923134	21.58580931263858
50	19.405764966740577	31.59379157427938	28.425129342202517	20.575314116777534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	190.0
2	323.0
3	950.0
4	1577.0
5	1168.0
6	759.0
7	732.5
8	706.0
9	734.5
10	763.0
11	744.0
12	725.0
13	684.0
14	643.0
15	587.0
16	531.0
17	567.0
18	603.0
19	489.0
20	375.0
21	482.0
22	589.0
23	604.5
24	620.0
25	851.5
26	1083.0
27	1474.5
28	1866.0
29	2016.0
30	2166.0
31	2518.5
32	2871.0
33	3243.0
34	3615.0
35	4036.5
36	4458.0
37	4943.0
38	5428.0
39	6961.5
40	8495.0
41	10814.5
42	13134.0
43	15212.0
44	17290.0
45	17603.0
46	17916.0
47	16077.5
48	14239.0
49	14178.0
50	14117.0
51	12980.0
52	11843.0
53	10465.5
54	9088.0
55	8825.5
56	8563.0
57	7799.5
58	7036.0
59	6412.5
60	5789.0
61	5142.0
62	4495.0
63	3704.0
64	2913.0
65	2297.5
66	1682.0
67	1340.5
68	999.0
69	848.5
70	698.0
71	537.5
72	377.0
73	338.5
74	300.0
75	251.0
76	202.0
77	145.5
78	89.0
79	65.0
80	41.0
81	37.0
82	33.0
83	25.5
84	18.0
85	12.5
86	7.0
87	4.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	169125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.60960827790096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.62108874467822	19.632224685883223
2	7.398834373579135	4.233555062823355
3	4.0114909271276815	3.4430155210643014
4	2.856198073823007	3.2685883222468592
5	1.975778117637333	2.826311899482631
6	1.723639069152234	2.958758314855876
7	1.3826313396436984	2.7689578713968954
8	1.147025999255983	2.625277161862528
9	0.9258876534534782	2.3840354767184038
>10	9.845823171991897	52.996895787139685
>50	0.0971355350721283	1.6573540280857353
>100	0.012400281073037657	0.7526977087952698
>500	0.0020667135121729425	0.4523281596452328
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.4523281596452328	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	457	0.2702143385070214	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	304	0.17974870657797487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020694752402069475	0.0
2	0.0	0.0	0.0	0.0892830746489283	0.0
3	0.0	0.0	0.0	0.14072431633407242	0.0
4	0.0	0.0	0.0	0.21167775314116777	0.0
5	0.0	0.0	0.0	0.4150776053215078	0.0
6	0.0	0.0	0.0	0.5936437546193644	0.0
7	0.0	0.0	0.0	0.7302291204730229	0.0
8	0.0	0.0	0.0	1.0424242424242425	0.0
9	0.0	0.0	0.0	1.1967479674796748	0.0
10	0.0	0.0	0.0	1.4569105691056912	0.0
11	0.0	0.0	0.0	1.665631929046563	0.0
12	0.0	0.0	0.0	1.8483370288248337	0.0
13	0.0	0.0	0.0	1.9127864005912787	0.0
14	0.0	0.0	0.0	1.9370288248337029	0.0
15	0.0	0.0	0.0	1.9683665927568366	0.0
16	0.0	0.0	0.0	2.0611973392461196	0.0
17	0.0	0.0	0.0	2.1670362158167036	0.0
18	0.0	0.0	0.0	2.327864005912786	0.0
19	0.0	0.0	0.0	2.38640059127864	0.0
20	0.0	0.0	0.0	2.4626755358462677	0.0
21	0.0	0.0	0.0	2.5495934959349595	0.0
22	0.0	0.0	0.0	2.663118994826312	0.0
23	0.0	0.0	0.0	2.7719142645971915	0.0
24	0.0	0.0	0.0	2.8582409460458242	0.0
25	0.0	0.0	0.0	2.925646711012565	0.0
26	0.0	0.0	0.0	2.9859571322985956	0.0
27	0.0	0.0	0.0	3.0557280118255727	0.0
28	0.0	0.0	0.0	3.126681448632668	0.0
29	0.0	0.0	0.0	3.2011825572801182	0.0
30	0.0	0.0	0.0	3.296378418329638	0.0
31	0.0	0.0	0.0	3.3963045084996306	0.0
32	0.0	0.0	0.0	3.496230598669623	0.0
33	0.0	0.0	0.0	3.5819660014781967	0.0
34	0.0	0.0	0.0	3.6830746489283075	0.0
35	0.0	0.0	0.0	3.8119733924611974	0.0
36	0.0	0.0	0.0	3.91189948263119	0.0
37	0.0	0.0	0.0	4.0118255728011825	0.0
38	0.0	0.0	0.0	4.1182557280118255	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTTA	20	7.8374264E-4	44.0	17
TTCTACG	20	7.8374264E-4	44.0	1
CAAACGC	20	7.8374264E-4	44.0	34
CGACGGT	20	7.8374264E-4	44.0	28
ATAGCGG	30	2.5148802E-6	44.0	2
CGACCCC	20	7.8374264E-4	44.0	20
AGGGTAC	35	1.4363104E-7	44.0	6
TGAGTAG	30	2.5148802E-6	44.0	1
CACGACG	20	7.8374264E-4	44.0	26
TGCGGGT	20	7.8374264E-4	44.0	4
GGCTTAA	25	4.4267006E-5	44.0	8
ACCTAGG	20	7.8374264E-4	44.0	2
CGAGGGC	35	1.4363104E-7	44.0	4
ACCAGAA	45	4.7475623E-10	44.0	2
GACGGTC	20	7.8374264E-4	44.0	29
CGGGACT	20	7.8374264E-4	44.0	6
GCGATAA	25	4.4267006E-5	44.0	9
TATAGCG	20	7.8374264E-4	44.0	1
CGTAAAT	20	7.8374264E-4	44.0	16
CGGTCTA	20	7.8374264E-4	44.0	31
>>END_MODULE