FastQCFastQC Report
Thu 26 May 2016
SRR1545830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545830_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5984288
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433910.7250820816110454No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC126030.21060149511520834No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA100190.16742175510269558No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT79020.13204578389275382No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC254.4450935E-544.032
CGTTTTT258950.041.9185181
ACGGGTA7850.039.796185
CTATGCG1000.039.61
CACGACG9500.039.36842326
TACGGGA14900.039.2751664
CGACGGT9650.038.9844628
CGGTCTA9700.038.78350431
GCGCGAC17500.037.7142879
ATAGCGG10300.037.592232
GGGCGAT74450.037.5580947
TCGTCCC22200.037.16216338
CTTAACG2250.037.1555561
TCGCTAT2850.037.05262823
AGGGCGA40200.036.995026
TAGGGCG23500.036.6978725
TAGCGGG40650.036.693733
GTACGTC301.30175E-436.66666410
CACGCTA1150.036.34782436
ACGGTCT10350.036.34782430
Produced by FastQC (version 0.11.5)