##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545830_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5984288
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.668819415108363	31.0	31.0	33.0	30.0	34.0
2	32.089106005593315	33.0	31.0	34.0	30.0	34.0
3	32.14174117288473	33.0	31.0	34.0	30.0	34.0
4	35.85236856915977	37.0	35.0	37.0	35.0	37.0
5	35.844702828473494	37.0	35.0	37.0	35.0	37.0
6	35.89949898801662	37.0	35.0	37.0	35.0	37.0
7	36.071518616751064	37.0	35.0	37.0	35.0	37.0
8	36.004613247223396	37.0	35.0	37.0	35.0	37.0
9	37.80933354143384	39.0	38.0	39.0	35.0	39.0
10	37.483444479944815	39.0	37.0	39.0	35.0	39.0
11	37.294441711361486	39.0	37.0	39.0	34.0	39.0
12	36.80701898036993	39.0	35.0	39.0	33.0	39.0
13	36.62738390933057	39.0	35.0	39.0	33.0	39.0
14	37.71188635974739	40.0	35.0	41.0	33.0	41.0
15	37.82287667304782	40.0	35.0	41.0	33.0	41.0
16	37.87275963322621	40.0	35.0	41.0	33.0	41.0
17	37.77780080103096	40.0	35.0	41.0	33.0	41.0
18	37.68581592329781	39.0	36.0	41.0	33.0	41.0
19	37.61268558598784	39.0	36.0	41.0	33.0	41.0
20	37.484213995048364	39.0	35.0	41.0	33.0	41.0
21	37.40485969258164	39.0	35.0	41.0	33.0	41.0
22	37.43854356608505	39.0	35.0	41.0	33.0	41.0
23	37.43295693656455	39.0	35.0	41.0	33.0	41.0
24	37.401097507339216	39.0	35.0	41.0	33.0	41.0
25	37.317517973733885	39.0	35.0	41.0	33.0	41.0
26	37.21989048655412	39.0	35.0	41.0	33.0	41.0
27	37.1226570312124	39.0	35.0	41.0	32.0	41.0
28	37.075390422386086	39.0	35.0	41.0	32.0	41.0
29	36.99490281884829	39.0	35.0	41.0	32.0	41.0
30	36.86971917126983	39.0	35.0	41.0	32.0	41.0
31	36.73187353282462	39.0	35.0	41.0	31.0	41.0
32	36.58243587207033	39.0	35.0	41.0	31.0	41.0
33	36.38954325059222	39.0	35.0	41.0	30.0	41.0
34	36.17771270366667	39.0	35.0	41.0	30.0	41.0
35	35.93995743520365	39.0	35.0	41.0	29.0	41.0
36	35.81986779379602	38.0	35.0	41.0	29.0	41.0
37	35.77047962932265	38.0	35.0	41.0	29.0	41.0
38	35.65682734520799	38.0	35.0	41.0	28.0	41.0
39	35.564638767385524	38.0	35.0	41.0	27.0	41.0
40	35.441960848141	38.0	35.0	41.0	26.0	41.0
41	35.32225170312659	38.0	35.0	40.0	26.0	41.0
42	35.3013461250528	38.0	35.0	40.0	26.0	41.0
43	35.230383631269085	38.0	35.0	40.0	26.0	41.0
44	35.10311636070991	38.0	34.0	40.0	25.0	41.0
45	34.98929613681695	38.0	34.0	40.0	24.0	41.0
46	34.91777250693817	38.0	34.0	40.0	24.0	41.0
47	34.83850760524894	37.0	34.0	40.0	24.0	41.0
48	34.764072685004464	37.0	34.0	40.0	24.0	41.0
49	34.700531625483265	37.0	34.0	40.0	24.0	41.0
50	34.55239253191023	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	5.0
12	19.0
13	32.0
14	43.0
15	124.0
16	306.0
17	858.0
18	1980.0
19	3843.0
20	7001.0
21	11332.0
22	17436.0
23	25598.0
24	37448.0
25	56440.0
26	83141.0
27	104043.0
28	109467.0
29	113308.0
30	124795.0
31	144665.0
32	176819.0
33	231443.0
34	413786.0
35	713920.0
36	366988.0
37	498558.0
38	857403.0
39	1883480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.48050762262778	22.807909646059816	28.61276061579924	15.098822115513158
2	32.328891924987566	24.974834098893638	29.5356774272896	13.1605965488292
3	28.712722382345234	24.27867776417178	33.52661168713805	13.481988166344935
4	25.413833692496084	26.4932937719575	33.0794072745162	15.013465261030218
5	22.608570977867377	31.10667467875878	31.996588399488797	14.288165943885053
6	19.865504467699417	41.42920594730735	28.30816297611345	10.397126608879786
7	85.76046139490613	3.8415263436519096	8.578831767455043	1.8191804939869205
8	86.90153281392874	3.0825722291440516	8.138111000005347	1.8777839569218593
9	82.46105468186023	4.325209615579999	9.746038292274703	3.4676974102850666
10	42.01283427535573	24.423072552657892	19.85511058291312	13.708982589073255
11	35.74131124705228	22.869871904560743	25.53869399333722	15.850122855049756
12	33.12161780983803	21.80125689137956	28.516742509718785	16.560382789063627
13	22.498633087177623	29.835596147778986	29.366484367062544	18.299286397980847
14	18.299620606494873	31.765382949483715	31.522346518082017	18.41264992593939
15	18.17880422867349	23.54704519568577	41.006148099824074	17.268002475816672
16	20.612627600810658	21.20985821538001	38.07401983327006	20.10349435053928
17	20.227903469886478	21.651982658588622	31.13125571496559	26.98885815655931
18	22.369294392248502	23.11533134768915	34.41582356998861	20.099550690073738
19	26.058455074354708	25.005664834312785	28.634300354528392	20.30157973680411
20	26.814651968621835	24.232339753701694	29.36685199642798	19.586156281248496
21	23.242079926634545	26.29174932757247	31.037627199760436	19.428543546032547
22	22.25718414621756	22.9554125737264	30.029503927618457	24.757899352437583
23	19.983095733360425	26.98127162329086	30.718424648011595	22.317207995337125
24	21.00462076691496	23.798303156532576	35.919862145672134	19.277213930880333
25	20.667270692854355	25.968669957060893	32.34170882150057	21.022350528584184
26	19.939932703773618	28.54474918319439	29.09519060579972	22.42012750723227
27	18.907997075007085	28.761633798373342	30.80262848312113	21.52774064349844
28	17.551812345929875	26.418397643963658	35.081299563122634	20.948490446983836
29	19.204256212267858	25.113129581998727	34.34923252356838	21.33338168216503
30	20.960404980508958	27.377208449860703	32.60663591057115	19.05575065905919
31	25.34067544877519	25.65613486516692	29.241206305578878	19.761983380479016
32	26.407084685763788	25.48393392831361	28.74124707901759	19.367734306905014
33	25.040990674245624	25.890782662866492	28.01145265735874	21.056774005529146
34	20.126020004384813	26.58877714441551	31.298092605168737	21.987110246030937
35	20.83038115812608	26.202632627306706	31.522262965953512	21.444723248613702
36	25.48390050746221	27.82553246100455	27.242455577004314	19.448111454528927
37	21.1620831082996	29.437386703313745	29.848730542380313	19.551799646006344
38	21.939903293424383	29.209222550786322	27.318621697351464	21.532252458437828
39	21.034114668277997	28.284300488211798	28.648036324454974	22.03354851905523
40	22.950366025164566	25.764752632226255	29.330439978824547	21.95444136378463
41	19.08925506259057	25.59275222048137	29.225247849034	26.09274486789406
42	21.316220074969657	26.315544973771317	27.81306648343128	24.55516846782775
43	21.185761781518536	25.62154428396494	28.924142688319815	24.268551246196708
44	20.623823586030618	27.412200081279508	29.596486666417128	22.367489666272746
45	20.013776074948264	30.5231466132646	27.417731232186686	22.04534607960045
46	21.578339812522394	29.80306094893829	27.64840194856932	20.970197289970002
47	20.90561149463395	27.61733726719035	29.50929166510703	21.967759573068676
48	21.04043120919314	26.577681421749755	30.862217861172457	21.519669507884647
49	20.95679552855745	25.925423375345574	31.629293242571215	21.48848785352577
50	20.417700485003394	28.990432947077416	29.31080522862536	21.28106133929383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3015.0
1	8114.0
2	13213.0
3	49811.0
4	86409.0
5	60727.0
6	35045.0
7	33909.0
8	32773.0
9	32151.0
10	31529.0
11	30476.5
12	29424.0
13	27955.0
14	26486.0
15	25271.0
16	24056.0
17	22524.5
18	20993.0
19	19843.0
20	18693.0
21	20811.0
22	22929.0
23	25827.5
24	28726.0
25	31333.5
26	33941.0
27	44661.0
28	55381.0
29	65371.0
30	75361.0
31	93346.5
32	111332.0
33	131520.0
34	151708.0
35	168962.0
36	186216.0
37	203879.5
38	221543.0
39	256315.5
40	291088.0
41	348174.0
42	405260.0
43	466768.5
44	528277.0
45	536848.0
46	545419.0
47	529930.0
48	514441.0
49	496671.0
50	478901.0
51	457008.5
52	435116.0
53	400473.0
54	365830.0
55	339404.0
56	312978.0
57	291883.0
58	270788.0
59	243163.5
60	215539.0
61	186334.5
62	157130.0
63	129921.0
64	102712.0
65	79920.5
66	57129.0
67	46478.5
68	35828.0
69	29910.5
70	23993.0
71	19780.0
72	15567.0
73	12309.5
74	9052.0
75	7002.0
76	4952.0
77	3611.0
78	2270.0
79	1914.0
80	1558.0
81	1259.0
82	960.0
83	663.5
84	367.0
85	260.5
86	154.0
87	119.0
88	84.0
89	78.0
90	72.0
91	49.5
92	27.0
93	19.0
94	11.0
95	10.5
96	10.0
97	5.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5984288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.48422479833762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27158507199718	11.481874583033518
2	8.328118084030999	2.4125266895241153
3	2.7880843639609036	1.2114972205301966
4	1.4399402243120856	0.8342567162041977
5	0.8859374958940668	0.6416058923902986
6	0.5628026043765361	0.4891055663327772
7	0.4439031983180514	0.4500715599197792
8	0.33650203830833536	0.3899176934365397
9	0.28367963302448485	0.3697991617892903
>10	3.000203273262194	10.152818419226849
>50	0.9718830571831335	10.09698039494551
>100	1.6061048421656694	52.54675324239631
>500	0.07644180588349207	6.533927425637579
>1k	0.004109774509865064	0.9513588817801297
>5k	3.5226638655986263E-4	0.31477823594376964
>10k+	3.5226638655986263E-4	1.122728316909232
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43391	0.7250820816110454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	12603	0.21060149511520834	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	10019	0.16742175510269558	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	7902	0.13204578389275382	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033337299274366475	0.0
2	0.0	0.0	0.0	0.14218901229352598	0.0
3	0.0	0.0	0.0	0.20119352544529942	0.0
4	1.671042570143683E-5	0.0	0.0	0.31555967894593306	0.0
5	1.671042570143683E-5	0.0	0.0	0.649166617649418	0.0
6	1.671042570143683E-5	0.0	0.0	0.8451297798501677	0.0
7	1.671042570143683E-5	0.0	0.0	1.012735349635579	0.0
8	1.671042570143683E-5	0.0	0.0	1.337853392082734	0.0
9	1.671042570143683E-5	0.0	0.0	1.4740600719751455	0.0
10	1.671042570143683E-5	0.0	0.0	1.925893272516296	0.0
11	1.671042570143683E-5	0.0	0.0	2.1438473549401365	0.0
12	1.671042570143683E-5	0.0	0.0	2.4218419969092397	0.0
13	1.671042570143683E-5	0.0	0.0	2.5073826660748946	0.0
14	1.671042570143683E-5	0.0	0.0	2.5391157644819233	0.0
15	1.671042570143683E-5	0.0	0.0	2.5952795052644526	0.0
16	1.671042570143683E-5	0.0	0.0	2.7010230791031447	0.0
17	1.671042570143683E-5	0.0	0.0	2.820068151800181	0.0
18	1.671042570143683E-5	0.0	0.0	2.99694800784989	0.0
19	1.671042570143683E-5	0.0	0.0	3.0815194723248616	0.0
20	1.671042570143683E-5	0.0	0.0	3.1739448368795085	0.0
21	1.671042570143683E-5	0.0	0.0	3.292856226170933	0.0
22	1.671042570143683E-5	0.0	0.0	3.4164465346587596	0.0
23	1.671042570143683E-5	0.0	0.0	3.555042805426477	0.0
24	1.671042570143683E-5	0.0	0.0	3.6636605724858162	0.0
25	1.671042570143683E-5	0.0	0.0	3.7552504157553916	0.0
26	1.671042570143683E-5	0.0	0.0	3.8468402590249666	0.0
27	1.671042570143683E-5	0.0	0.0	3.951046473699127	0.0
28	1.671042570143683E-5	0.0	0.0	4.05633886604388	0.0
29	3.342085140287366E-5	0.0	0.0	4.178675892604099	0.0
30	3.342085140287366E-5	0.0	0.0	4.3101033907459	0.0
31	3.342085140287366E-5	0.0	0.0	4.420576015058098	0.0
32	5.013127710431049E-5	0.0	0.0	4.5280240523183375	0.0
33	5.013127710431049E-5	0.0	0.0	4.642256522413359	0.0
34	5.013127710431049E-5	0.0	0.0	4.766782614740467	0.0
35	5.013127710431049E-5	0.0	0.0	4.9109935885438665	0.0
36	5.013127710431049E-5	0.0	0.0	5.034065873834949	0.0
37	5.013127710431049E-5	0.0	0.0	5.165409819848243	0.0
38	5.013127710431049E-5	0.0	0.0	5.31750811458272	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	25	4.4450935E-5	44.0	32
CGTTTTT	25895	0.0	41.918518	1
ACGGGTA	785	0.0	39.79618	5
CTATGCG	100	0.0	39.6	1
CACGACG	950	0.0	39.368423	26
TACGGGA	1490	0.0	39.275166	4
CGACGGT	965	0.0	38.98446	28
CGGTCTA	970	0.0	38.783504	31
GCGCGAC	1750	0.0	37.714287	9
ATAGCGG	1030	0.0	37.59223	2
GGGCGAT	7445	0.0	37.558094	7
TCGTCCC	2220	0.0	37.162163	38
CTTAACG	225	0.0	37.155556	1
TCGCTAT	285	0.0	37.052628	23
AGGGCGA	4020	0.0	36.99502	6
TAGGGCG	2350	0.0	36.697872	5
TAGCGGG	4065	0.0	36.69373	3
GTACGTC	30	1.30175E-4	36.666664	10
CACGCTA	115	0.0	36.347824	36
ACGGTCT	1035	0.0	36.347824	30
>>END_MODULE