FastQCFastQC Report
Thu 26 May 2016
SRR1545829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545829_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1921395
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113100.5886348200135839No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA58090.3023324199344747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC454.802132E-1044.012
TCACGCA207.857803E-444.043
CGAACGA207.857803E-444.022
GCGACTA600.044.09
CGTATAG207.857803E-444.012
CTAAGCG1050.044.01
TAACGGC207.857803E-444.010
CGTTAGT254.4439614E-544.016
CCGTACA408.3182385E-944.039
CGCTATA254.4439614E-544.025
CGTAAAT207.857803E-444.016
ACCGTTG302.528608E-644.042
ACCAACG302.528608E-644.018
CGGTAAG302.528608E-644.030
GCTAACG302.528608E-644.01
CAATCGA254.4439614E-544.025
TATCGTA207.857803E-444.026
ACGAACG207.857803E-444.021
TTTAGCG1450.042.4827581
TAATGCG1100.042.01
Produced by FastQC (version 0.11.5)