FastQCFastQC Report
Thu 26 May 2016
SRR1545828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545828_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3046151
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183860.6035813720331001No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA77720.255141652531342No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA40430.13272487148535972No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA33820.11102535626106519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATC207.8585313E-444.022
CCGTACC254.444577E-544.032
ATGCGTC254.444577E-544.029
TTACGCA502.7284841E-1144.01
ACCCGTA302.5290992E-644.034
TCGACGA302.5290992E-644.05
TTCGATC254.444577E-544.031
TAATGCG900.041.5555571
TAAGACG850.041.4117661
TAGGGAC27400.041.2700735
CGTTTTT112500.041.2035561
TATACGG3700.041.0270272
ACGGGTA2900.040.965525
ACGTATG1450.040.965521
CGTAAGG3950.040.6582262
ACTACGG1300.040.6153832
TCACGAC6850.040.1459925
GCGGCTA10350.039.96135324
AGGGCGA21650.039.9353376
ATATACG1600.039.8751
Produced by FastQC (version 0.11.5)