FastQCFastQC Report
Thu 26 May 2016
SRR1545827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545827_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2591777
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149080.5752038080436704No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA68650.2648761834062113No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA42280.16313131878244155No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA33010.12736435272016072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG800.044.0000041
TCGTTAG800.044.0000041
GCGACTA207.8583125E-444.0000049
GGTCGAA207.8583125E-444.0000049
CGCGGAC207.8583125E-444.0000044
CTATCGT551.8189894E-1244.00000439
ATATCGT302.5289519E-644.00000414
ACGTCGT207.8583125E-444.00000424
ACGTATA207.8583125E-444.00000413
TAGTGCG1600.044.0000041
TCGACCA207.8583125E-444.00000433
CGTATTG254.4443932E-544.039
GTCGACC254.4443932E-544.012
CGCTAGC351.4470243E-744.043
TGCTCGA254.4443932E-544.02
CGTAAAC254.4443932E-544.041
CACGTTA254.4443932E-544.042
ATCGCTA850.044.028
GCGTAAA254.4443932E-544.019
ACACGTA454.802132E-1043.99999638
Produced by FastQC (version 0.11.5)