##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545827_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2591777 Sequences flagged as poor quality 0 Sequence length 50 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.726477239361255 31.0 31.0 33.0 30.0 34.0 2 32.15736268976845 33.0 31.0 34.0 30.0 34.0 3 32.22829356075002 34.0 31.0 34.0 30.0 34.0 4 35.916458862008575 37.0 35.0 37.0 35.0 37.0 5 35.90212969711514 37.0 35.0 37.0 35.0 37.0 6 35.95029742142167 37.0 35.0 37.0 35.0 37.0 7 36.11331453284754 37.0 35.0 37.0 35.0 37.0 8 36.057015707755724 37.0 35.0 37.0 35.0 37.0 9 37.81939804234701 39.0 38.0 39.0 35.0 39.0 10 37.55901337190661 39.0 37.0 39.0 35.0 39.0 11 37.33160260315606 39.0 37.0 39.0 35.0 39.0 12 36.63873743767307 39.0 35.0 39.0 33.0 39.0 13 36.42042930391002 39.0 35.0 39.0 33.0 39.0 14 37.45816441769489 40.0 35.0 41.0 33.0 41.0 15 37.63137646487333 40.0 35.0 41.0 33.0 41.0 16 37.69399836482845 40.0 35.0 41.0 33.0 41.0 17 37.62157971152611 39.0 35.0 41.0 33.0 41.0 18 37.54426518948197 39.0 35.0 41.0 33.0 41.0 19 37.484271602070706 39.0 35.0 41.0 33.0 41.0 20 37.348760329303026 39.0 35.0 41.0 33.0 41.0 21 37.238871631317046 39.0 35.0 41.0 33.0 41.0 22 37.26306892915556 39.0 35.0 41.0 33.0 41.0 23 37.25774208197696 39.0 35.0 41.0 33.0 41.0 24 37.21871519038868 39.0 35.0 41.0 33.0 41.0 25 37.124333613578635 39.0 35.0 41.0 33.0 41.0 26 37.0196444370021 38.0 35.0 41.0 33.0 41.0 27 36.938195299981444 38.0 35.0 41.0 33.0 41.0 28 36.92619079496423 38.0 35.0 41.0 33.0 41.0 29 36.88164259502264 38.0 35.0 41.0 32.0 41.0 30 36.78771437511792 38.0 35.0 41.0 32.0 41.0 31 36.64137385276588 38.0 35.0 41.0 32.0 41.0 32 36.46945126837687 38.0 35.0 41.0 31.0 41.0 33 36.310539062581384 38.0 35.0 41.0 31.0 41.0 34 36.118913780004995 38.0 35.0 41.0 30.0 41.0 35 35.913666183471804 38.0 35.0 41.0 30.0 41.0 36 35.77921441543775 38.0 35.0 41.0 30.0 41.0 37 35.73537075141881 38.0 35.0 41.0 29.0 41.0 38 35.67111252241223 38.0 35.0 41.0 29.0 41.0 39 35.64928155470166 38.0 35.0 41.0 29.0 41.0 40 35.48789729980627 38.0 35.0 40.0 28.0 41.0 41 35.406179621163396 38.0 35.0 40.0 28.0 41.0 42 35.394792453208744 38.0 35.0 40.0 28.0 41.0 43 35.314646283225755 38.0 35.0 40.0 27.0 41.0 44 35.19015679203882 37.0 34.0 40.0 27.0 41.0 45 35.066517682655565 37.0 34.0 40.0 27.0 41.0 46 35.02296532456303 37.0 34.0 40.0 27.0 41.0 47 34.96218193154735 37.0 34.0 40.0 26.0 41.0 48 34.908669997457345 37.0 34.0 40.0 26.0 41.0 49 34.853228499211156 36.0 34.0 40.0 26.0 41.0 50 34.715787662287305 36.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 9.0 13 12.0 14 25.0 15 68.0 16 135.0 17 324.0 18 679.0 19 1476.0 20 2644.0 21 4300.0 22 6914.0 23 10201.0 24 15339.0 25 22893.0 26 32822.0 27 41143.0 28 43560.0 29 45577.0 30 50978.0 31 60817.0 32 75867.0 33 101588.0 34 203812.0 35 405363.0 36 144907.0 37 191505.0 38 331324.0 39 797490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.0020765675442 23.4577666211252 28.693209330895364 13.846947480435237 2 32.016064653710565 25.11639697396805 29.558098555547023 13.30943981677436 3 28.402327823728662 24.137879146238276 33.15177193099561 14.308021099037454 4 25.648232853366626 27.149480838822164 32.53169543521684 14.670590872594364 5 23.370992180268598 31.707704790960022 30.895713635856787 14.025589392914592 6 21.550619517034068 40.131384760340104 27.778933141238614 10.539062581387212 7 85.98822352386027 4.526122424884548 7.466383103175929 2.0192709480792526 8 86.60752834831084 4.040123822381323 7.340484925979357 2.0118629033284883 9 81.98919119970583 5.910037784886586 8.983797602957354 3.116973412450222 10 46.466266194969705 26.50023516683727 15.98339671970235 11.050101918490672 11 41.1374898380532 20.84357566256665 24.34368388947043 13.675250609909725 12 39.19743095181414 20.695762019649067 26.726836452364537 13.379970576172257 13 22.03488185904883 36.53774996845793 25.560802491881052 15.866565680612183 14 15.218670433451642 38.34608455897247 29.17565824528885 17.259586762287032 15 15.395460334743305 24.16415455496364 44.443947145144044 15.996437965149008 16 17.265104212283696 20.598454265162474 42.28469501812849 19.851746504425343 17 16.50284727428324 20.86606988178381 31.588635905018066 31.042446938914885 18 21.26093410042608 23.092843250017268 34.70190529509291 20.944317354463752 19 25.601778239408713 26.212401761416977 27.900239874032373 20.285580125141937 20 28.69872678089203 22.746555741485473 28.384116380383034 20.170601097239462 21 21.526581955160495 27.287918675102063 29.531475894723968 21.654023475013474 22 21.705686870436768 25.09216649426243 27.94395505477516 25.25819158052564 23 19.43211163614771 28.316363637766674 28.708874258857918 23.542650467227695 24 19.865636588333025 23.245016835939204 39.10035469872601 17.788991877001763 25 17.593527529567552 26.295549347031017 34.206337968119946 21.90458515528149 26 16.88405291041629 31.39355739324795 30.091979363965343 21.630410332370417 27 17.239137472089613 31.560199816573725 30.52627598747886 20.6743867238578 28 15.646060598577732 27.9897537481041 36.73093788547394 19.633247767844225 29 16.6158585402988 23.960819159981742 36.55931818208125 22.864004117638206 30 18.597201842596796 29.92464243644418 32.259604124891915 19.218551596067098 31 26.15560675166112 27.774496031101442 27.070461694814018 18.99943552242342 32 27.14600831784525 26.13342120097524 28.30841542308617 18.412155058093347 33 25.86221731267775 27.972468310352316 26.94317450922668 19.222139867743252 34 19.384190846666204 26.717769314258135 30.697857107305143 23.200182731770518 35 20.23144738146839 26.81970709671395 31.381364986262323 21.567480535555337 36 25.74882021099809 27.573591400803387 28.00356666487896 18.674021723319562 37 19.098981123761803 32.47933753559816 30.47333933436403 17.948342006276004 38 19.861855398824822 31.100399455663048 28.653854093156934 20.3838910523552 39 20.685768875948817 31.061352886455897 29.080781255486098 19.172096982109185 40 22.541445502448706 27.609319783299256 27.868292681044704 21.98094203320733 41 17.463346576499443 26.36843370397993 29.737859391452275 26.430360328068346 42 21.010333836591652 26.409525202206822 26.679224331414314 25.900916629787208 43 21.573846824013025 26.194383235903395 27.574440239264415 24.657329700819165 44 19.57008646963068 29.600193226500583 28.689582475652802 22.14013782821593 45 17.91257504021372 35.20576808884407 24.74780816405115 22.13384870689106 46 20.84697101641075 32.815207481199195 27.08404311019042 19.25377839219964 47 20.20933899791533 28.34086420243717 28.873471753163948 22.576325046483554 48 21.226131723524052 24.839984304205185 32.3368870084116 21.59699696385916 49 20.043622580183403 26.249673486569254 32.21747087037195 21.489233062875392 50 18.7349065911149 30.55166397417679 30.07075840243972 20.642671032268595 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1321.0 1 3551.5 2 5782.0 3 17512.0 4 29242.0 5 21040.5 6 12839.0 7 12520.0 8 12201.0 9 12152.0 10 12103.0 11 11587.5 12 11072.0 13 10320.0 14 9568.0 15 9179.0 16 8790.0 17 8624.5 18 8459.0 19 8304.0 20 8149.0 21 8525.5 22 8902.0 23 12730.0 24 16558.0 25 16574.5 26 16591.0 27 22482.5 28 28374.0 29 35162.5 30 41951.0 31 48722.5 32 55494.0 33 67603.5 34 79713.0 35 87539.0 36 95365.0 37 102620.5 38 109876.0 39 122735.5 40 135595.0 41 170235.5 42 204876.0 43 233042.0 44 261208.0 45 261049.5 46 260891.0 47 238491.0 48 216091.0 49 205530.5 50 194970.0 51 188182.5 52 181395.0 53 160034.5 54 138674.0 55 127186.5 56 115699.0 57 105561.5 58 95424.0 59 84535.5 60 73647.0 61 65578.0 62 57509.0 63 46381.5 64 35254.0 65 27707.0 66 20160.0 67 15892.5 68 11625.0 69 8730.0 70 5835.0 71 4989.0 72 4143.0 73 3508.5 74 2874.0 75 2261.5 76 1649.0 77 1200.0 78 751.0 79 621.5 80 492.0 81 355.5 82 219.0 83 188.5 84 158.0 85 124.5 86 91.0 87 92.5 88 94.0 89 74.0 90 54.0 91 43.5 92 33.0 93 20.0 94 7.0 95 6.5 96 6.0 97 4.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2591777.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.218664248769356 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25633421386212 11.411378700239263 2 7.656501270358143 2.1773044176699696 3 2.520489559783183 1.0751398437925668 4 1.2454946622185679 0.7083708170288086 5 0.7297097396932951 0.5187748893877924 6 0.4908206670772619 0.41872885629171547 7 0.3973366489800469 0.3954717483904888 8 0.2943518066265879 0.3348231607553709 9 0.25474950917640937 0.32599779646663374 >10 3.125595658584147 10.617678332051558 >50 1.1079587968842532 11.354171931478966 >100 1.8871496430839458 56.540241837750415 >500 0.03048662645579827 2.616222137386274 >1k 0.0024718886315512113 0.655412597312463 >5k 2.74654292394579E-4 0.26809315858607274 >10k+ 2.74654292394579E-4 0.5821897754116784 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14908 0.5752038080436704 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 6865 0.2648761834062113 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA 4228 0.16313131878244155 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 3301 0.12736435272016072 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03144560662433535 0.0 2 0.0 0.0 0.0 0.10162911392453904 0.0 3 0.0 0.0 0.0 0.14866248137860627 0.0 4 0.0 0.0 0.0 0.2196176600070145 0.0 5 0.0 0.0 0.0 0.3697463169092094 0.0 6 0.0 0.0 0.0 0.5091873259157713 0.0 7 0.0 0.0 0.0 0.6155236349423581 0.0 8 0.0 0.0 0.0 0.9084886546952149 0.0 9 0.0 0.0 0.0 1.0388625255953734 0.0 10 0.0 0.0 0.0 1.2623385422434106 0.0 11 0.0 0.0 0.0 1.4404788683594305 0.0 12 0.0 0.0 0.0 1.606118118958537 0.0 13 0.0 0.0 0.0 1.6664628168241327 0.0 14 0.0 0.0 0.0 1.6888027017756544 0.0 15 0.0 0.0 0.0 1.7256885912638318 0.0 16 0.0 0.0 0.0 1.8116913607922287 0.0 17 0.0 0.0 0.0 1.9157126558342017 0.0 18 0.0 0.0 0.0 2.0735966095848526 0.0 19 0.0 0.0 0.0 2.1436643661858255 0.0 20 0.0 0.0 0.0 2.219249572783461 0.0 21 0.0 0.0 0.0 2.315939990207491 0.0 22 0.0 0.0 0.0 2.4191896139212594 0.0 23 0.0 0.0 0.0 2.53798841489835 0.0 24 0.0 0.0 0.0 2.626769201208283 0.0 25 0.0 0.0 0.0 2.7030489120013024 0.0 26 0.0 0.0 0.0 2.7768592745440674 0.0 27 0.0 0.0 0.0 2.8490877108640134 3.858356641022742E-5 28 0.0 0.0 0.0 2.928917109766774 3.858356641022742E-5 29 0.0 0.0 0.0 3.0152285478264527 3.858356641022742E-5 30 0.0 0.0 0.0 3.1246515421658576 3.858356641022742E-5 31 0.0 0.0 0.0 3.2211876253242466 3.858356641022742E-5 32 0.0 0.0 0.0 3.316604785056739 3.858356641022742E-5 33 0.0 0.0 0.0 3.4129865339494874 3.858356641022742E-5 34 0.0 0.0 0.0 3.5104100391353112 3.858356641022742E-5 35 0.0 0.0 0.0 3.6405138250705984 3.858356641022742E-5 36 0.0 0.0 0.0 3.742065771862317 3.858356641022742E-5 37 0.0 0.0 0.0 3.851295848369671 3.858356641022742E-5 38 0.0 0.0 0.0 3.9592912507518974 3.858356641022742E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACG 80 0.0 44.000004 1 TCGTTAG 80 0.0 44.000004 1 GCGACTA 20 7.8583125E-4 44.000004 9 GGTCGAA 20 7.8583125E-4 44.000004 9 CGCGGAC 20 7.8583125E-4 44.000004 4 CTATCGT 55 1.8189894E-12 44.000004 39 ATATCGT 30 2.5289519E-6 44.000004 14 ACGTCGT 20 7.8583125E-4 44.000004 24 ACGTATA 20 7.8583125E-4 44.000004 13 TAGTGCG 160 0.0 44.000004 1 TCGACCA 20 7.8583125E-4 44.000004 33 CGTATTG 25 4.4443932E-5 44.0 39 GTCGACC 25 4.4443932E-5 44.0 12 CGCTAGC 35 1.4470243E-7 44.0 43 TGCTCGA 25 4.4443932E-5 44.0 2 CGTAAAC 25 4.4443932E-5 44.0 41 CACGTTA 25 4.4443932E-5 44.0 42 ATCGCTA 85 0.0 44.0 28 GCGTAAA 25 4.4443932E-5 44.0 19 ACACGTA 45 4.802132E-10 43.999996 38 >>END_MODULE