FastQCFastQC Report
Thu 26 May 2016
SRR1545826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545826_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences715937
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.6092714861782531No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA19950.27865580351343766No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17430.24345717570121392No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12770.17836764966749868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG302.5263726E-644.0000041
CCATGCG302.5263726E-644.0000041
ACATAGC302.5263726E-644.00000432
ACAACGT254.4411536E-544.010
ACAACGC207.8544905E-444.010
CGGAATA254.4411536E-544.031
AACGTCG207.8544905E-444.014
AACGTAA207.8544905E-444.016
CGGAACA254.4411536E-544.014
CTCACCG207.8544905E-444.019
AAATCGT207.8544905E-444.012
AGTTTCG207.8544905E-444.010
CTCGTAG207.8544905E-444.01
GATCGAC207.8544905E-444.09
CGAAATC254.4411536E-544.016
GGTCGGA207.8544905E-444.09
CCGATCA207.8544905E-444.038
TAGACCC207.8544905E-444.030
GCGCGAC502.7284841E-1144.09
CGTGATG254.4411536E-544.02
Produced by FastQC (version 0.11.5)