FastQCFastQC Report
Thu 26 May 2016
SRR1545825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545825_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1573475
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96430.6128473601423601No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA76210.4843419819190009No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA38530.24487201893897267No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC23950.15221087084319737No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20140.12799694942722317No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA16790.10670649358903064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA254.4435914E-544.034
ACTATCG351.4465331E-744.029
ATTACTC207.85737E-444.043
CTATACG302.5283152E-644.01
CACGCTA207.85737E-444.030
GTTAGTC254.4435914E-544.043
CGCGATT454.802132E-1044.027
CCGCTTA254.4435914E-544.016
TATGCGT207.85737E-444.019
TCGATGC254.4435914E-544.032
ACTTACG850.044.01
CCGCTAA207.85737E-444.036
CGTTACA207.85737E-444.016
ACGATTG800.044.01
TACGCCA302.5283152E-644.014
TGCGCGT502.7284841E-1144.037
CGCTCAC207.85737E-444.030
TACGATG302.5283152E-644.035
TACGATA351.4465331E-744.031
TGCGATA302.5283152E-644.019
Produced by FastQC (version 0.11.5)