##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545825_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1573475
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70451198779771	31.0	31.0	33.0	30.0	34.0
2	32.123506887621346	33.0	31.0	34.0	30.0	34.0
3	32.173850871478734	33.0	31.0	34.0	30.0	34.0
4	35.873896312302385	37.0	35.0	37.0	35.0	37.0
5	35.85132525143393	37.0	35.0	37.0	35.0	37.0
6	35.89635361222771	37.0	35.0	37.0	35.0	37.0
7	36.06909102464291	37.0	35.0	37.0	35.0	37.0
8	36.000008261967935	37.0	35.0	37.0	35.0	37.0
9	37.72255485470058	39.0	38.0	39.0	35.0	39.0
10	37.56432609351912	39.0	37.0	39.0	35.0	39.0
11	37.29755604633058	39.0	37.0	39.0	34.0	39.0
12	36.36774591270913	38.0	35.0	39.0	33.0	39.0
13	36.08299845882521	38.0	35.0	39.0	33.0	39.0
14	37.03473585535201	39.0	35.0	41.0	33.0	41.0
15	37.24382402008294	39.0	35.0	41.0	33.0	41.0
16	37.34184909197795	39.0	35.0	41.0	33.0	41.0
17	37.28250337628497	38.0	35.0	41.0	33.0	41.0
18	37.22649549563864	38.0	35.0	41.0	33.0	41.0
19	37.12473537870001	38.0	35.0	41.0	33.0	41.0
20	36.94623174820064	38.0	35.0	41.0	33.0	41.0
21	36.79676512178459	38.0	35.0	41.0	32.0	41.0
22	36.78921050541	38.0	35.0	41.0	33.0	41.0
23	36.7837760371153	38.0	35.0	41.0	33.0	41.0
24	36.76431465387121	38.0	35.0	41.0	33.0	41.0
25	36.67722652091708	38.0	35.0	41.0	33.0	41.0
26	36.56762897408602	37.0	35.0	41.0	32.0	41.0
27	36.465083334657365	37.0	35.0	41.0	32.0	41.0
28	36.464593971941085	37.0	35.0	41.0	32.0	41.0
29	36.435637680929155	37.0	35.0	41.0	32.0	41.0
30	36.3618163618742	37.0	35.0	40.0	32.0	41.0
31	36.165728085924464	37.0	35.0	40.0	31.0	41.0
32	35.97149875276061	37.0	35.0	40.0	31.0	41.0
33	35.811366561273616	37.0	35.0	40.0	30.0	41.0
34	35.62914695181048	37.0	35.0	40.0	30.0	41.0
35	35.4463820524635	37.0	35.0	40.0	29.0	41.0
36	35.25999459794404	36.0	35.0	40.0	28.0	41.0
37	35.188902905988336	36.0	34.0	40.0	28.0	41.0
38	35.13282003209457	36.0	34.0	40.0	28.0	41.0
39	35.09424553933174	36.0	35.0	40.0	27.0	41.0
40	34.918420693052006	36.0	34.0	40.0	26.0	41.0
41	34.869294396161365	36.0	34.0	40.0	26.0	41.0
42	34.84108613101574	36.0	34.0	40.0	26.0	41.0
43	34.741704825307046	35.0	34.0	40.0	26.0	41.0
44	34.616262095044405	35.0	34.0	40.0	26.0	41.0
45	34.50131651281399	35.0	34.0	40.0	25.0	41.0
46	34.46128282940625	35.0	34.0	40.0	25.0	41.0
47	34.41587505362335	35.0	34.0	40.0	25.0	41.0
48	34.365302276807704	35.0	34.0	40.0	24.0	41.0
49	34.31889670951239	36.0	34.0	40.0	24.0	41.0
50	34.1416978344111	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	8.0
13	10.0
14	19.0
15	43.0
16	98.0
17	244.0
18	599.0
19	1370.0
20	2380.0
21	3349.0
22	4932.0
23	7453.0
24	10452.0
25	15513.0
26	21973.0
27	27464.0
28	29096.0
29	30012.0
30	33319.0
31	39753.0
32	49968.0
33	67627.0
34	138851.0
35	301659.0
36	85412.0
37	107888.0
38	181813.0
39	412162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.383371200686376	23.137450547355375	29.32731692591239	14.151861326045854
2	32.634264923179586	25.09935016444494	29.269483150351927	12.996901762023546
3	28.65644512941102	24.529020162379446	32.12685298463592	14.68768172357362
4	27.134463528178077	27.340377190613136	31.16986288310904	14.355296398099748
5	23.45204086496449	31.96701568185068	30.27007102114746	14.31087243203737
6	22.224375983094742	40.606237785792594	26.960580879899588	10.20880535121308
7	84.40451866092566	5.466562862454123	8.302705794499436	1.8262126821207834
8	85.13208026819619	4.331876896677735	8.422758543987035	2.1132842911390393
9	80.0748025866315	6.780469978868428	9.643432529909912	3.501294904590159
10	52.71281717218259	22.06657239549405	15.011360205913663	10.209250226409699
11	48.07880646340107	18.829152036098446	20.967222231049114	12.124819269451374
12	43.628656318022216	20.374521361953637	23.659162045790367	12.337660274233782
13	21.168115159122326	40.85702028948665	23.513497195697422	14.461367355693607
14	14.14093010692893	42.672492413289056	28.907608954702173	14.278968525079838
15	14.206962296827086	21.776132445701393	50.064983555506124	13.951921701965395
16	15.720618376523301	16.729913090452662	48.400006355359956	19.149462177664088
17	15.383657191884206	17.547212380241188	30.000413098396862	37.06871732947775
18	20.965252069464086	22.141629196523617	36.47499960279001	20.418119131222294
19	29.3064077916713	22.728991563259665	27.01542763628275	20.949173008786286
20	30.62425523125566	21.555347240979362	26.490284243473837	21.330113284291137
21	21.277554457490584	27.595608446273374	29.664850092947137	21.4619870032889
22	22.304898393682773	24.320882123961297	26.47776418436899	26.896455297986936
23	18.40258027614039	29.060963790336675	27.80387359189056	24.732582341632376
24	19.00799186513926	22.462447766885397	41.63809402755049	16.891466340424856
25	16.63375649438345	24.08706843133828	38.31646514879486	20.962709925483406
26	15.638507125947346	33.967174565849476	29.327444033111426	21.066874275091756
27	16.746055704729976	33.91245491666534	30.289899744196763	19.05158963440792
28	14.654570298225266	27.539681278698424	40.24944787810419	17.556300544972117
29	15.386008675066334	24.554632262984793	38.79635837874768	21.263000683201195
30	17.863455091437743	31.176536011058325	31.97934508015698	18.980663817346954
31	29.325855193123502	27.11603298431815	24.73201035923672	18.826101463321628
32	29.94168957244316	26.440712435850582	27.056101939973626	16.56149605173263
33	27.943882171626495	27.800695911914712	25.600470296636423	18.65495161982237
34	18.716344396955783	27.920303786205693	29.510287738921814	23.853064077916713
35	20.699598023483055	26.136608462161774	32.20114714247128	20.962646371883885
36	29.027280382592668	26.320341918365404	26.148810753269036	18.503566945772892
37	20.340837954209633	33.50660163014983	28.55386961979059	17.59869079584995
38	20.50544177695864	33.93927453566151	24.62447766885397	20.930806018525875
39	21.513783186895246	31.201512575668506	27.003098237976452	20.281605999459796
40	24.38252911549278	26.350402770936938	25.789288040801413	23.477780072768873
41	17.05626082397242	25.595767330272167	27.748899728308363	29.59907211744705
42	21.942833537234467	25.271262651138404	24.66693147333132	28.118972338295812
43	22.18535407299131	26.812246778626925	26.190120592955086	24.812278555426683
44	19.82224058215097	30.08811706573031	28.99118193806702	21.0984604140517
45	17.39557349179364	38.09104053130809	22.69607079870986	21.817315178188405
46	21.032237563354993	33.71683693735204	26.076041881822082	19.174883617470883
47	22.660035907783726	27.821033063760147	26.651329064649897	22.867601963806226
48	22.35485152290313	24.481545623540253	32.39587537139135	20.76772748216527
49	20.347129760561813	25.26884761435676	33.024484024213926	21.359538600867506
50	19.40424855812771	33.722111886111946	27.331161918683165	19.54247763707717
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1025.0
1	2294.5
2	3564.0
3	11285.0
4	19006.0
5	13953.5
6	8901.0
7	8668.5
8	8436.0
9	8586.5
10	8737.0
11	8386.0
12	8035.0
13	7395.5
14	6756.0
15	6453.5
16	6151.0
17	5345.5
18	4540.0
19	4564.0
20	4588.0
21	5468.0
22	6348.0
23	5525.5
24	4703.0
25	7300.5
26	9898.0
27	13402.0
28	16906.0
29	20316.0
30	23726.0
31	26079.0
32	28432.0
33	32875.5
34	37319.0
35	40038.0
36	42757.0
37	45561.0
38	48365.0
39	58215.5
40	68066.0
41	100048.0
42	132030.0
43	152939.0
44	173848.0
45	173545.5
46	173243.0
47	155887.0
48	138531.0
49	128849.0
50	119167.0
51	113549.5
52	107932.0
53	97283.0
54	86634.0
55	79747.5
56	72861.0
57	66637.0
58	60413.0
59	54868.0
60	49323.0
61	41698.0
62	34073.0
63	28512.5
64	22952.0
65	18034.0
66	13116.0
67	10504.5
68	7893.0
69	6951.5
70	6010.0
71	4809.5
72	3609.0
73	2922.5
74	2236.0
75	1937.5
76	1639.0
77	1248.5
78	858.0
79	616.0
80	374.0
81	319.5
82	265.0
83	175.5
84	86.0
85	64.5
86	43.0
87	34.5
88	26.0
89	24.5
90	23.0
91	20.0
92	17.0
93	12.0
94	7.0
95	6.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1573475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.46294984753865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.81652248434375	11.688453115435435
2	7.27466202924765	2.1042614417360506
3	2.304002164684616	0.9996800326936226
4	1.117926777211564	0.6467407564812546
5	0.7177565848293047	0.5190438744563428
6	0.4921715388334535	0.42709513695204954
7	0.35970358523632007	0.3641662439276919
8	0.3013444827188202	0.34866641123158193
9	0.26660839138867354	0.34703494142286073
>10	3.3290936741477095	11.637216751362727
>50	1.0854289554696346	11.35362572033103
>100	1.9139227656961193	55.947711660881836
>500	0.016418998917402366	1.531919889636995
>1k	0.0035500538199788904	0.9763751008021954
>5k	8.875134549947226E-4	1.1080089226483338
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9643	0.6128473601423601	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7621	0.4843419819190009	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3853	0.24487201893897267	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	2395	0.15221087084319737	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2014	0.12799694942722317	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	1679	0.10670649358903064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02980663817346955	0.0
2	0.0	0.0	0.0	0.10613451119337772	0.0
3	0.0	0.0	0.0	0.15189310284561242	0.0
4	0.0	0.0	0.0	0.2123961295857894	0.0
5	0.0	0.0	0.0	0.3941594242043884	0.0
6	0.0	0.0	0.0	0.5559033349751347	0.0
7	0.0	0.0	0.0	0.6528225742385484	0.0
8	0.0	0.0	0.0	0.8856829628688094	0.0
9	0.0	0.0	0.0	0.9839368277220801	0.0
10	0.0	0.0	0.0	1.148667757670125	0.0
11	0.0	0.0	0.0	1.4448275314193109	0.0
12	0.0	0.0	0.0	1.580323805589539	0.0
13	0.0	0.0	0.0	1.668472648119608	0.0
14	0.0	0.0	0.0	1.6904621935524873	0.0
15	0.0	0.0	0.0	1.728340138864615	0.0
16	0.0	0.0	0.0	1.8106420502391205	0.0
17	0.0	0.0	0.0	1.90088816155325	0.0
18	0.0	0.0	0.0	2.0521457284036924	0.0
19	0.0	0.0	0.0	2.098666963250131	0.0
20	0.0	0.0	0.0	2.158598007594655	0.0
21	0.0	0.0	0.0	2.2293967174565847	0.0
22	0.0	0.0	0.0	2.3055339296779422	0.0
23	0.0	0.0	0.0	2.391966825021052	0.0
24	0.0	0.0	0.0	2.454376459746739	0.0
25	0.0	0.0	0.0	2.506172643353088	0.0
26	0.0	0.0	0.0	2.5619727037290074	0.0
27	0.0	0.0	0.0	2.6163745849155533	0.0
28	6.355359951699264E-5	0.0	0.0	2.676114968461526	0.0
29	6.355359951699264E-5	0.0	0.0	2.740240550374172	0.0
30	6.355359951699264E-5	0.0	0.0	2.8212078361588206	0.0
31	6.355359951699264E-5	0.0	0.0	2.8972179411811436	0.0
32	6.355359951699264E-5	0.0	0.0	2.9687792942372773	0.0
33	6.355359951699264E-5	0.0	0.0	3.0373536281161124	0.0
34	6.355359951699264E-5	0.0	0.0	3.109614070766933	0.0
35	6.355359951699264E-5	0.0	0.0	3.2114269371931554	0.0
36	6.355359951699264E-5	0.0	0.0	3.29417372376428	0.0
37	6.355359951699264E-5	0.0	0.0	3.3746325807527926	0.0
38	6.355359951699264E-5	0.0	0.0	3.453439044153863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	25	4.4435914E-5	44.0	34
ACTATCG	35	1.4465331E-7	44.0	29
ATTACTC	20	7.85737E-4	44.0	43
CTATACG	30	2.5283152E-6	44.0	1
CACGCTA	20	7.85737E-4	44.0	30
GTTAGTC	25	4.4435914E-5	44.0	43
CGCGATT	45	4.802132E-10	44.0	27
CCGCTTA	25	4.4435914E-5	44.0	16
TATGCGT	20	7.85737E-4	44.0	19
TCGATGC	25	4.4435914E-5	44.0	32
ACTTACG	85	0.0	44.0	1
CCGCTAA	20	7.85737E-4	44.0	36
CGTTACA	20	7.85737E-4	44.0	16
ACGATTG	80	0.0	44.0	1
TACGCCA	30	2.5283152E-6	44.0	14
TGCGCGT	50	2.7284841E-11	44.0	37
CGCTCAC	20	7.85737E-4	44.0	30
TACGATG	30	2.5283152E-6	44.0	35
TACGATA	35	1.4465331E-7	44.0	31
TGCGATA	30	2.5283152E-6	44.0	19
>>END_MODULE