FastQCFastQC Report
Thu 26 May 2016
SRR1545824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545824_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3216406
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210470.6543639080389727No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA114500.3559873971134241No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC54680.17000341374814No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT35120.10919019551636204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT650.044.00000425
AACGCGA650.044.00000423
CGAACAC254.4446315E-544.039
TCGATTG750.044.01
CACGAAG502.7284841E-1144.01
TATGCGA502.7284841E-1144.012
TTATCCG302.5291429E-644.023
CCCGTTA254.4446315E-544.019
TAATTCG207.858597E-444.020
CGCTAAT254.4446315E-544.035
CTACCGT207.858597E-444.040
TCACGAC2750.043.225
CGGTCTA2650.043.1698131
CGTAATC1050.041.90476233
CGTTTTT135650.041.5672721
TATCGTG900.041.5555571
CGATACA850.041.41176610
AACGAGC3800.041.10526315
TCGTTGA4800.040.79166424
AGTACGG2650.040.6792452
Produced by FastQC (version 0.11.5)