FastQCFastQC Report
Thu 26 May 2016
SRR1545823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545823_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2022962
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125870.6222064477731168No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA55190.2728177790784009No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA39510.1953076726107559No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA33900.16757605926359467No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA30540.1509667507348136No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20530.10148485240948667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG207.857901E-444.00000441
GCGCATA207.857901E-444.00000436
CGCCTAC207.857901E-444.00000415
TACGCCC207.857901E-444.00000424
ACGGATC207.857901E-444.00000436
AGCGCAT207.857901E-444.00000435
AACGACA207.857901E-444.00000423
CGACTCG207.857901E-444.00000436
AACCGAT351.446806E-744.08
TCGTTAC302.5286754E-644.044
TAACGCC254.4440443E-544.012
GCGCATC302.5286754E-644.020
ATCGTAC454.802132E-1044.028
CGACTTA502.7284841E-1144.043
ATACGTC302.5286754E-644.01
TAGTCCG254.4440443E-544.034
AAGTCGT302.5286754E-644.030
CGCATGG3100.043.2903252
CTTAACG1050.041.9047621
GCGTATG950.041.684211
Produced by FastQC (version 0.11.5)