##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545823_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2022962
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739955570099685	31.0	31.0	33.0	30.0	34.0
2	32.16654638099974	33.0	31.0	34.0	30.0	34.0
3	32.225847544343395	34.0	31.0	34.0	30.0	34.0
4	35.91160684184874	37.0	35.0	37.0	35.0	37.0
5	35.902852352145025	37.0	35.0	37.0	35.0	37.0
6	35.96322273972522	37.0	35.0	37.0	35.0	37.0
7	36.10524962900934	37.0	35.0	37.0	35.0	37.0
8	36.04324648708182	37.0	35.0	37.0	35.0	37.0
9	37.762753329029415	39.0	38.0	39.0	35.0	39.0
10	37.5855656211041	39.0	37.0	39.0	35.0	39.0
11	37.35453953163727	39.0	37.0	39.0	35.0	39.0
12	36.72713427142971	39.0	35.0	39.0	33.0	39.0
13	36.54062755504058	39.0	35.0	39.0	33.0	39.0
14	37.602270334291994	40.0	35.0	41.0	33.0	41.0
15	37.74027094923187	40.0	35.0	41.0	33.0	41.0
16	37.80382478761341	40.0	35.0	41.0	33.0	41.0
17	37.75091820805334	40.0	35.0	41.0	33.0	41.0
18	37.65636329303269	39.0	35.0	41.0	33.0	41.0
19	37.58332336445272	39.0	36.0	41.0	33.0	41.0
20	37.4447251110006	39.0	35.0	41.0	33.0	41.0
21	37.334742323385214	39.0	35.0	41.0	33.0	41.0
22	37.339308894581315	39.0	35.0	41.0	33.0	41.0
23	37.30690344158714	39.0	35.0	41.0	33.0	41.0
24	37.2825836570336	39.0	35.0	41.0	33.0	41.0
25	37.20071360707715	39.0	35.0	41.0	33.0	41.0
26	37.10424812725103	39.0	35.0	41.0	33.0	41.0
27	37.01060524122549	39.0	35.0	41.0	33.0	41.0
28	36.95546530285789	39.0	35.0	41.0	32.0	41.0
29	36.903897354473294	38.0	35.0	41.0	32.0	41.0
30	36.82677282123935	38.0	35.0	41.0	32.0	41.0
31	36.687833483772806	38.0	35.0	41.0	32.0	41.0
32	36.514659197750625	38.0	35.0	41.0	31.0	41.0
33	36.35947437470402	38.0	35.0	41.0	31.0	41.0
34	36.18577956481634	38.0	35.0	41.0	30.0	41.0
35	35.97549039477756	38.0	35.0	41.0	30.0	41.0
36	35.836507556741054	38.0	35.0	41.0	29.0	41.0
37	35.767832514896476	38.0	35.0	41.0	29.0	41.0
38	35.68923538850458	38.0	35.0	41.0	29.0	41.0
39	35.64294732179843	38.0	35.0	41.0	29.0	41.0
40	35.51548224830719	38.0	35.0	41.0	28.0	41.0
41	35.437129318296634	38.0	35.0	40.0	27.0	41.0
42	35.41915765100877	38.0	35.0	40.0	27.0	41.0
43	35.34237914503584	38.0	35.0	40.0	27.0	41.0
44	35.206528348036194	38.0	34.0	40.0	27.0	41.0
45	35.07680915410176	37.0	34.0	40.0	26.0	41.0
46	35.04860298908235	37.0	34.0	40.0	26.0	41.0
47	34.98445447813651	37.0	34.0	40.0	26.0	41.0
48	34.91612793517624	37.0	34.0	40.0	26.0	41.0
49	34.85200957803458	37.0	34.0	40.0	26.0	41.0
50	34.71057637266543	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	9.0
12	6.0
13	23.0
14	27.0
15	62.0
16	123.0
17	313.0
18	661.0
19	1266.0
20	2247.0
21	3745.0
22	5562.0
23	7985.0
24	11738.0
25	17700.0
26	25666.0
27	32302.0
28	34477.0
29	35691.0
30	39736.0
31	46298.0
32	58235.0
33	77724.0
34	153422.0
35	297704.0
36	114439.0
37	152192.0
38	267569.0
39	636034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.581519573773505	23.42560067860889	27.105551167051082	14.887328580566518
2	33.00541483231025	25.43888614813328	28.77972992077953	12.775969098776944
3	30.33077240205204	24.223292380183118	32.14430127703832	13.301633940726518
4	27.233581253627108	26.08249685362355	31.374143458947817	15.309778433801524
5	22.698844565543	32.137430164283856	30.59434631001472	14.569378960158422
6	21.65008536986854	41.071359719065406	27.359535176637028	9.91901973442902
7	84.68260896645612	5.092977525035073	7.9888796724802535	2.235533836028556
8	85.46977155280227	4.412292470150205	8.079835409661674	2.038100567385843
9	79.86818338653914	6.836361730966771	9.853917176892102	3.441537705601984
10	45.04750954293753	27.988711602096334	16.100203562894407	10.863575292071724
11	39.354619612231964	21.898681240675803	24.443266853257747	14.303432293834486
12	36.27176387890628	22.120286985123794	27.642783206011778	13.96516592995815
13	22.03521371137965	35.85776697733324	26.71142611675355	15.39559319453356
14	15.609438041841617	38.07753185675262	29.79210682158142	16.520923279824338
15	15.43825341257028	24.054332211875458	45.08057986259752	15.426834512956745
16	17.40803831213834	20.465040865819525	42.6784091841567	19.44851163788544
17	17.505815729608365	20.791641167753028	31.024557060389668	30.67798604224894
18	21.989587545391363	23.77953713416268	34.00948707884775	20.221388241598213
19	26.46510413937583	25.140758946534834	27.858555919488353	20.535580994600984
20	28.11140298235953	23.726990422954064	27.95920041997823	20.202406174708177
21	21.894430048611888	26.391449765245216	29.54178081446908	22.172339371673814
22	22.322614067886594	25.28035623012197	28.13162086089605	24.265408841095386
23	19.08903874615539	28.41135918519478	28.91942606929839	23.580175999351447
24	19.719450983261176	24.773624022596568	37.68661991673595	17.8203050774063
25	17.97053034115322	26.725761531852797	34.637625422524	20.66608270446998
26	18.127972744915624	31.3484385767009	29.2836939102168	21.23989476816668
27	19.152559464784805	30.176197081309486	30.8284090358593	19.842834418046408
28	16.902887943520444	27.600617312633652	35.95495120521295	19.541543538632954
29	17.060923536873158	25.221185568488185	36.29420621840648	21.423684676232178
30	18.953050032575995	29.075583228948442	32.79097679541187	19.180389943063687
31	26.328621101137838	27.113806388849614	27.527556128093362	19.030016381919186
32	26.223873705981624	27.008317506705517	28.480268042602873	18.287540744709986
33	24.887862451197797	28.30641406017513	27.312821496399835	19.49290199222724
34	19.302784728531726	27.50990873778153	30.788269873581413	22.39903666010533
35	21.375092562292323	26.242608610542362	30.983429248794593	21.398869578370725
36	25.877945309897072	28.161576935206888	27.24341831433314	18.717059440562895
37	20.87211722217224	32.33862030033189	29.082800368963923	17.706462108531944
38	20.56123644438205	31.79802685369275	26.72353707088912	20.917199631036073
39	21.602926797438606	30.25088953722314	27.742043597457588	20.404140067880665
40	22.02132318847314	27.9882667099036	27.443520936132266	22.546889165491
41	18.215369344555164	27.89988146094687	27.97585916097287	25.9088900335251
42	21.397880929053535	28.163949693568146	25.94853487114439	24.48963450623393
43	22.07446308927207	27.486280019100708	27.57199591490102	22.867260976726207
44	19.856181183828465	30.29463726950877	28.162961044250956	21.68622050241181
45	17.68629366246128	34.70411208910499	25.356877687272426	22.252716561161307
46	20.556985252318135	32.276681420609975	27.566212316395465	19.600121010676425
47	21.394371223977515	28.09019645450582	29.409054643636413	21.106377677880257
48	21.474896710862588	25.850955183537806	31.416358784791804	21.257789320807806
49	20.04965985520242	26.482158340097346	32.20698164374813	21.26120016095211
50	19.02388675615261	31.127722616638376	29.368421156699924	20.479969470509086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1116.0
1	2773.5
2	4431.0
3	14804.5
4	25178.0
5	17983.0
6	10788.0
7	10621.5
8	10455.0
9	10276.5
10	10098.0
11	9885.0
12	9672.0
13	9171.0
14	8670.0
15	8185.0
16	7700.0
17	7399.0
18	7098.0
19	7309.0
20	7520.0
21	8025.0
22	8530.0
23	9785.5
24	11041.0
25	12913.5
26	14786.0
27	20523.0
28	26260.0
29	33241.0
30	40222.0
31	42480.0
32	44738.0
33	51515.5
34	58293.0
35	62919.5
36	67546.0
37	71230.5
38	74915.0
39	88733.0
40	102551.0
41	125427.0
42	148303.0
43	173288.0
44	198273.0
45	200809.0
46	203345.0
47	187032.0
48	170719.0
49	160241.0
50	149763.0
51	144721.0
52	139679.0
53	128531.5
54	117384.0
55	105737.0
56	94090.0
57	84516.5
58	74943.0
59	69123.0
60	63303.0
61	55438.5
62	47574.0
63	36950.5
64	26327.0
65	21080.5
66	15834.0
67	12220.5
68	8607.0
69	6853.0
70	5099.0
71	4332.5
72	3566.0
73	2722.5
74	1879.0
75	1457.5
76	1036.0
77	857.5
78	679.0
79	597.0
80	515.0
81	360.5
82	206.0
83	141.0
84	76.0
85	70.0
86	64.0
87	47.0
88	30.0
89	36.5
90	43.0
91	24.5
92	6.0
93	5.5
94	5.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2022962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.615041881061034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.35490102503177	11.743902438293533
2	6.959591950701272	2.0342945566918864
3	2.2428852265405985	0.9833958456091172
4	1.1498403511631847	0.6721985955513547
5	0.7258925083331614	0.53044747052188
6	0.5030646426917325	0.44113864930924046
7	0.40290315505730867	0.41219125396219347
8	0.3345444159456313	0.3911504520096402
9	0.2960154747009127	0.3893650704176402
>10	3.7295181152853956	13.15517097075115
>50	1.28200712295603	13.599731524319699
>100	2.0044622003560786	52.78285625426027
>500	0.010267008026409756	0.9525848891792205
>1k	0.0034223360088032517	1.0059536514136247
>5k	3.422336008803252E-4	0.2760470466463701
>10k+	3.422336008803252E-4	0.6295713310632107
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12587	0.6222064477731168	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5519	0.2728177790784009	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3951	0.1953076726107559	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3390	0.16757605926359467	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3054	0.1509667507348136	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2053	0.10148485240948667	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02120652785371154	0.0
2	0.0	0.0	0.0	0.07622486235529881	0.0
3	0.0	0.0	0.0	0.1102838313324719	0.0
4	0.0	0.0	0.0	0.17355738763259024	0.0
5	0.0	0.0	0.0	0.33984820278383876	0.0
6	0.0	0.0	0.0	0.4644674492155562	0.0
7	0.0	0.0	0.0	0.5602675680512041	0.0
8	0.0	0.0	0.0	0.7763863087887959	0.0
9	0.0	0.0	0.0	0.8750040781784334	0.0
10	0.0	0.0	0.0	1.0853392204104675	0.0
11	0.0	0.0	0.0	1.2255296935879172	0.0
12	0.0	0.0	0.0	1.3899915075023654	0.0
13	0.0	0.0	0.0	1.4378915669201893	0.0
14	0.0	0.0	0.0	1.4566264714809274	0.0
15	0.0	0.0	0.0	1.4853961666111375	0.0
16	0.0	0.0	0.0	1.5475822086623476	0.0
17	0.0	0.0	0.0	1.6242519632103816	0.0
18	0.0	0.0	0.0	1.7483768849835044	0.0
19	0.0	0.0	0.0	1.8000832442725074	0.0
20	0.0	0.0	0.0	1.8582158241232412	0.0
21	0.0	0.0	0.0	1.930337791812204	0.0
22	0.0	0.0	0.0	2.0110610085607146	0.0
23	0.0	0.0	0.0	2.097963283541658	0.0
24	0.0	0.0	0.0	2.165488031905691	0.0
25	0.0	0.0	0.0	2.2191716898290723	0.0
26	0.0	0.0	0.0	2.273893429535503	0.0
27	0.0	0.0	0.0	2.3312845223983447	0.0
28	9.886493171893491E-5	0.0	0.0	2.391394400883457	0.0
29	9.886493171893491E-5	0.0	0.0	2.4564969584203755	0.0
30	9.886493171893491E-5	0.0	0.0	2.5408781776424867	0.0
31	9.886493171893491E-5	0.0	0.0	2.6194263658931805	0.0
32	9.886493171893491E-5	0.0	0.0	2.689818197277062	0.0
33	9.886493171893491E-5	0.0	0.0	2.7615447052391495	0.0
34	9.886493171893491E-5	0.0	0.0	2.8376212701968697	0.0
35	9.886493171893491E-5	0.0	0.0	2.9370299590402587	0.0
36	9.886493171893491E-5	0.0	0.0	3.024723153474954	0.0
37	9.886493171893491E-5	0.0	0.0	3.1101424544801137	0.0
38	9.886493171893491E-5	0.0	0.0	3.188591777799089	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	20	7.857901E-4	44.000004	41
GCGCATA	20	7.857901E-4	44.000004	36
CGCCTAC	20	7.857901E-4	44.000004	15
TACGCCC	20	7.857901E-4	44.000004	24
ACGGATC	20	7.857901E-4	44.000004	36
AGCGCAT	20	7.857901E-4	44.000004	35
AACGACA	20	7.857901E-4	44.000004	23
CGACTCG	20	7.857901E-4	44.000004	36
AACCGAT	35	1.446806E-7	44.0	8
TCGTTAC	30	2.5286754E-6	44.0	44
TAACGCC	25	4.4440443E-5	44.0	12
GCGCATC	30	2.5286754E-6	44.0	20
ATCGTAC	45	4.802132E-10	44.0	28
CGACTTA	50	2.7284841E-11	44.0	43
ATACGTC	30	2.5286754E-6	44.0	1
TAGTCCG	25	4.4440443E-5	44.0	34
AAGTCGT	30	2.5286754E-6	44.0	30
CGCATGG	310	0.0	43.290325	2
CTTAACG	105	0.0	41.904762	1
GCGTATG	95	0.0	41.68421	1
>>END_MODULE