FastQCFastQC Report
Thu 26 May 2016
SRR1545822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545822_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2010779
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA120240.5979772018705188No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99850.49657371595784516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC800.044.00000420
CTATGCG800.044.0000041
AGCCGAA800.044.00000411
GTACCGT207.85789E-444.00000428
ACAGTCG207.85789E-444.0000041
GTCGATA207.85789E-444.0000041
CGAGTTT207.85789E-444.00000417
ACGTTCG207.85789E-444.00000444
CACGAAC207.85789E-444.00000442
AAAGTCG207.85789E-444.00000419
GCCGAAT408.3182385E-944.00000440
TCGTGCA207.85789E-444.00000423
CTACGTA800.044.00000430
CGTAGGT408.3182385E-944.00000418
CTACCGA207.85789E-444.00000437
TACTCAC207.85789E-444.00000430
CGTGTAC207.85789E-444.00000413
TTACGCG207.85789E-444.0000041
ACGTATA207.85789E-444.00000413
CGGTCTA1750.044.00000431
Produced by FastQC (version 0.11.5)