##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545822_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2010779
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78350927675294	31.0	31.0	33.0	30.0	34.0
2	32.19907458751061	33.0	31.0	34.0	30.0	34.0
3	32.22996012988001	34.0	31.0	34.0	30.0	34.0
4	35.90176742446584	37.0	35.0	37.0	35.0	37.0
5	35.89339753399056	37.0	35.0	37.0	35.0	37.0
6	35.949978093067415	37.0	35.0	37.0	35.0	37.0
7	36.15238472253788	37.0	35.0	37.0	35.0	37.0
8	36.11396677606042	37.0	35.0	37.0	35.0	37.0
9	37.933699824794274	39.0	39.0	39.0	35.0	39.0
10	37.682598137338815	39.0	38.0	39.0	35.0	39.0
11	37.36500580123425	39.0	37.0	39.0	35.0	39.0
12	36.20704164903254	37.0	35.0	39.0	33.0	39.0
13	35.826501072469924	37.0	35.0	39.0	33.0	39.0
14	36.71326834028006	38.0	35.0	41.0	33.0	41.0
15	36.972818494722695	38.0	35.0	41.0	33.0	41.0
16	37.14121641413601	38.0	35.0	41.0	33.0	41.0
17	37.06403935986998	38.0	35.0	41.0	33.0	41.0
18	37.04452105378065	38.0	35.0	41.0	33.0	41.0
19	36.96604350851088	37.0	35.0	41.0	33.0	41.0
20	36.788459099682264	36.0	35.0	41.0	33.0	41.0
21	36.638806154231766	36.0	35.0	41.0	33.0	41.0
22	36.62263480969316	36.0	35.0	41.0	33.0	41.0
23	36.6226417721689	36.0	35.0	41.0	33.0	41.0
24	36.57371247660733	36.0	35.0	41.0	33.0	41.0
25	36.5033884877453	36.0	35.0	40.0	33.0	41.0
26	36.39860074130474	36.0	35.0	40.0	33.0	41.0
27	36.32447126213273	36.0	35.0	40.0	32.0	41.0
28	36.33616474013305	36.0	35.0	40.0	32.0	41.0
29	36.33656508248793	36.0	35.0	40.0	32.0	41.0
30	36.280395806799255	36.0	35.0	40.0	32.0	41.0
31	36.09852450219542	36.0	35.0	40.0	32.0	41.0
32	35.88452087474556	35.0	35.0	40.0	31.0	41.0
33	35.72151191155269	36.0	35.0	40.0	31.0	41.0
34	35.548139800544966	36.0	35.0	40.0	30.0	41.0
35	35.35234752302466	36.0	35.0	40.0	30.0	41.0
36	35.17053937802215	35.0	35.0	40.0	29.0	41.0
37	35.06512301948648	35.0	34.0	40.0	29.0	41.0
38	35.05651491287705	35.0	34.0	40.0	29.0	41.0
39	35.04147646260479	35.0	35.0	40.0	29.0	41.0
40	34.825239372402436	35.0	34.0	40.0	27.0	41.0
41	34.818907498039316	35.0	34.0	40.0	27.0	41.0
42	34.78952485578972	35.0	34.0	40.0	27.0	41.0
43	34.69399272620213	35.0	34.0	40.0	27.0	41.0
44	34.55891075051012	35.0	34.0	40.0	27.0	41.0
45	34.44044024728724	35.0	34.0	40.0	26.0	41.0
46	34.432352834399005	35.0	34.0	40.0	27.0	41.0
47	34.401486190178034	35.0	34.0	40.0	26.0	41.0
48	34.380449069738646	35.0	34.0	40.0	26.0	41.0
49	34.36456070010677	35.0	34.0	40.0	26.0	41.0
50	34.14330664881621	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	8.0
14	9.0
15	44.0
16	106.0
17	246.0
18	550.0
19	1237.0
20	2231.0
21	3662.0
22	5769.0
23	8692.0
24	12605.0
25	19353.0
26	27667.0
27	34065.0
28	35824.0
29	36694.0
30	41342.0
31	49916.0
32	64435.0
33	87456.0
34	191417.0
35	454711.0
36	101218.0
37	122671.0
38	210157.0
39	498683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.51595525913091	22.91017560855768	29.932926492667768	14.64094263964364
2	31.22580850506197	25.60380827530027	30.164876398649476	13.005506820988282
3	28.862843703858054	24.561824049286372	33.354834121502165	13.220498125353409
4	25.303725571034906	27.078659564278322	32.227111979983874	15.390502884702894
5	22.392217145693287	33.09100602303883	30.488631520420697	14.02814531084719
6	20.559444871863093	39.80362834503444	28.43176699179771	11.205159791304762
7	85.64034138013177	4.939677607534194	7.542947285604236	1.8770337267297896
8	88.30731771119551	3.280519639403435	6.974709801524683	1.4374528478763704
9	84.87243003830854	4.572009156650234	8.186478971582655	2.369081833458575
10	57.953957147951115	20.71654816367189	12.956371635072776	8.373123053304218
11	52.952761094083435	16.913444988235902	19.776216083418415	10.357577834262244
12	46.768143092801346	20.941784253764336	22.186774379481783	10.103298273952532
13	19.830374198258486	47.02933539687852	22.233721358737085	10.906569046125904
14	12.79116203222731	47.1758955111427	28.096722712938615	11.936219743691376
15	11.101219974945034	22.12719547996075	56.096816209041364	10.674768336052844
16	13.258940937815641	15.64677172379461	52.52218170171859	18.57210563667116
17	13.250685430870323	16.678511164081183	30.068346645752715	40.002456759295775
18	19.74985813955686	22.36650571743588	36.834778958801536	21.048857184205723
19	28.965788880826782	21.997892359130468	28.2702872866685	20.766031473374248
20	32.0598136344173	20.565213780330907	26.663596546413103	20.71137603883868
21	20.265528931821947	27.625512301451327	30.539457593300906	21.56950117342582
22	21.889178273693926	23.772378764647932	26.925634293972635	27.412808667685507
23	16.991524180429572	30.184271866774022	25.86216585711309	26.962038095683315
24	17.894209159733617	22.13102484161611	43.62851412313337	16.346251875516902
25	14.003975573645835	24.314656160622324	40.669014347175896	21.01235391855594
26	14.068179546335028	35.27239940341529	30.02940651359498	20.630014536654702
27	15.754640365748799	37.05782684223378	30.028660534051728	17.158872257965694
28	11.942137848067839	28.848520896627626	42.49487387723862	16.714467378065915
29	12.44139709038139	23.589365116703526	42.259293537479756	21.70994425543533
30	15.179738797749529	31.68383994461848	35.348141193040114	17.788280064591884
31	27.969756994677187	27.68499173703326	26.507637089903962	17.837614178385593
32	30.494052305101654	26.599840161449865	27.568469732377356	15.337637801071127
33	27.67529400297099	28.441862581616377	25.465752327829165	18.41709108758347
34	18.273763551340053	28.09771735232962	30.50971787550994	23.11880122082039
35	19.481753091712218	26.147627362330716	31.988199598265147	22.382419947691915
36	30.75574192887433	24.53939493101927	27.87128769496797	16.833575445138425
37	19.023870848064355	32.379689662563614	31.483768231118386	17.11267125825364
38	19.425705162029246	33.008102829798794	25.465553399950963	22.100638608220994
39	19.666954946316825	31.971688584374512	28.839121554382658	19.522234914926006
40	21.990084439911097	26.708454782947307	26.391512941004457	24.90994783613714
41	16.094160521867394	24.83231623166942	28.01511255090689	31.058410695556297
42	22.821851630636683	24.48986188934736	24.839378171345533	27.848908308670424
43	22.518387152441914	26.756396401593612	25.94601395777457	24.7792024881899
44	18.573000812123063	31.679115407511215	28.414311070485613	21.3335727098801
45	15.323364725810245	40.6466846928479	22.87879473577156	21.151155845570297
46	20.96704809429579	34.69620480420772	26.212278922745863	18.124468178750625
47	21.363859479336117	28.744978936024296	27.86541932256106	22.025742262078527
48	22.850049657371596	24.006815269107147	31.419017206764146	21.724117866757112
49	20.814172019898756	24.191917659772656	32.83762163818103	22.156288682147565
50	18.72622501030695	34.60385253675317	27.615615639510853	19.054306813429026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1468.0
1	2458.5
2	3449.0
3	11343.0
4	19237.0
5	14425.5
6	9614.0
7	10383.0
8	11152.0
9	11210.0
10	11268.0
11	10982.5
12	10697.0
13	9747.5
14	8798.0
15	8083.0
16	7368.0
17	7024.5
18	6681.0
19	6436.0
20	6191.0
21	6869.0
22	7547.0
23	8384.5
24	9222.0
25	11698.5
26	14175.0
27	15412.5
28	16650.0
29	21332.5
30	26015.0
31	30465.0
32	34915.0
33	38161.5
34	41408.0
35	49585.5
36	57763.0
37	62814.0
38	67865.0
39	86134.0
40	104403.0
41	147630.5
42	190858.0
43	222435.0
44	254012.0
45	251580.0
46	249148.0
47	222671.0
48	196194.0
49	181399.0
50	166604.0
51	149157.5
52	131711.0
53	122242.5
54	112774.0
55	93214.5
56	73655.0
57	62881.5
58	52108.0
59	45922.5
60	39737.0
61	33355.5
62	26974.0
63	21653.0
64	16332.0
65	13310.0
66	10288.0
67	7742.0
68	5196.0
69	4543.0
70	3890.0
71	3062.0
72	2234.0
73	1862.0
74	1490.0
75	1171.5
76	853.0
77	628.5
78	404.0
79	303.5
80	203.0
81	168.5
82	134.0
83	97.0
84	60.0
85	39.5
86	19.0
87	12.5
88	6.0
89	5.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2010779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.874680241373806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6436366869312	10.38261035845761
2	8.500394506095548	2.1887972238302162
3	2.7546606463873	1.0639612498719773
4	1.2724231523072722	0.6552816487070805
5	0.7181144589795968	0.4622747018034729
6	0.4753271068583782	0.36718107065153627
7	0.36477933442535	0.3287492102571279
8	0.2610339175738421	0.2688582576733075
9	0.21027208153983554	0.24364672321621167
>10	2.0896191779992326	6.25334282711288
>50	0.7610529910939515	7.106289969851587
>100	1.8195020731799132	59.63796516702886
>500	0.1252810307787971	9.429997794968232
>1k	0.0031222686798454105	0.505140767479169
>5k	3.902835849806763E-4	0.5017239195543124
>10k+	3.902835849806763E-4	0.6041791095364099
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	12024	0.5979772018705188	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9985	0.49657371595784516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041625658513441806	0.0
2	0.0	0.0	0.0	0.16610477829736633	0.0
3	0.0	0.0	0.0	0.22871732796095443	0.0
4	0.0	0.0	0.0	0.3255952046445681	0.0
5	0.0	0.0	0.0	0.5539644088186718	0.0
6	0.0	0.0	0.0	0.7173836607603322	0.0
7	0.0	0.0	0.0	0.8405200173664038	0.0
8	0.0	0.0	0.0	1.1111116636885505	0.0
9	0.0	0.0	0.0	1.2272855445576067	0.0
10	0.0	0.0	0.0	1.5348777762250352	0.0
11	0.0	0.0	0.0	1.7231630129417503	0.0
12	0.0	0.0	0.0	1.9463103603130927	0.0
13	0.0	0.0	0.0	2.024787408263166	0.0
14	0.0	0.0	0.0	2.0496533930382204	0.0
15	0.0	0.0	0.0	2.102070888944036	0.0
16	0.0	0.0	0.0	2.2065577569688166	0.0
17	0.0	0.0	0.0	2.33501543431675	0.0
18	0.0	0.0	0.0	2.555725915180137	0.0
19	0.0	0.0	0.0	2.6284837866319473	0.0
20	0.0	0.0	0.0	2.7084030616989736	0.0
21	0.0	0.0	0.0	2.8068226294386402	0.0
22	0.0	0.0	0.0	2.9006668559796975	0.0
23	0.0	0.0	0.0	3.016940200787854	0.0
24	0.0	0.0	0.0	3.0999428579669868	0.0
25	0.0	0.0	0.0	3.1698660071544413	0.0
26	0.0	0.0	0.0	3.2402864760373964	0.0
27	0.0	0.0	0.0	3.3095631096206994	0.0
28	0.0	0.0	0.0	3.377148856239298	0.0
29	0.0	0.0	0.0	3.454084213133318	0.0
30	0.0	0.0	0.0	3.558869472975399	0.0
31	0.0	0.0	0.0	3.660272958888073	0.0
32	0.0	4.973196955010968E-5	0.0	3.7498402360478202	0.0
33	0.0	4.973196955010968E-5	0.0	3.8311520062622497	0.0
34	0.0	4.973196955010968E-5	0.0	3.9249465008337565	0.0
35	0.0	4.973196955010968E-5	0.0	4.060565581796905	0.0
36	0.0	4.973196955010968E-5	0.0	4.162068531648679	0.0
37	0.0	4.973196955010968E-5	0.0	4.254719190920534	0.0
38	0.0	4.973196955010968E-5	0.0	4.340506838394473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	80	0.0	44.000004	20
CTATGCG	80	0.0	44.000004	1
AGCCGAA	80	0.0	44.000004	11
GTACCGT	20	7.85789E-4	44.000004	28
ACAGTCG	20	7.85789E-4	44.000004	1
GTCGATA	20	7.85789E-4	44.000004	1
CGAGTTT	20	7.85789E-4	44.000004	17
ACGTTCG	20	7.85789E-4	44.000004	44
CACGAAC	20	7.85789E-4	44.000004	42
AAAGTCG	20	7.85789E-4	44.000004	19
GCCGAAT	40	8.3182385E-9	44.000004	40
TCGTGCA	20	7.85789E-4	44.000004	23
CTACGTA	80	0.0	44.000004	30
CGTAGGT	40	8.3182385E-9	44.000004	18
CTACCGA	20	7.85789E-4	44.000004	37
TACTCAC	20	7.85789E-4	44.000004	30
CGTGTAC	20	7.85789E-4	44.000004	13
TTACGCG	20	7.85789E-4	44.000004	1
ACGTATA	20	7.85789E-4	44.000004	13
CGGTCTA	175	0.0	44.000004	31
>>END_MODULE