FastQCFastQC Report
Thu 26 May 2016
SRR1545821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545821_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1337139
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59110.4420632409943917No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45660.3414753439993898No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20620.15420984654549752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA254.4432323E-544.037
CGTATGT302.5280297E-644.022
CGAAACG207.856945E-444.032
CGACGGT1450.044.028
CGACGAA254.4432323E-544.010
CGCATCC254.4432323E-544.029
GAATCGT207.856945E-444.012
GCACGAC254.4432323E-544.013
ATCACGC207.856945E-444.029
ATCGTCA254.4432323E-544.020
CGTTACC207.856945E-444.038
AACGCGT207.856945E-444.019
CGAATTA408.314601E-944.016
ATGACGG600.044.02
CGTACGA207.856945E-444.022
TCCGTAC207.856945E-444.032
CGTAAGA207.856945E-444.025
TAGGCGA207.856945E-444.01
GACGAAT207.856945E-444.025
TTACCCG254.4432323E-544.029
Produced by FastQC (version 0.11.5)