##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545821_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1337139
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67493955377863	31.0	31.0	33.0	30.0	34.0
2	32.106557358659046	33.0	31.0	34.0	30.0	34.0
3	32.198232943620674	33.0	31.0	34.0	30.0	34.0
4	35.87671364009277	37.0	35.0	37.0	35.0	37.0
5	35.84169559036121	37.0	35.0	37.0	35.0	37.0
6	35.897807931710915	37.0	35.0	37.0	35.0	37.0
7	36.060748358996335	37.0	35.0	37.0	35.0	37.0
8	36.00152414969573	37.0	35.0	37.0	35.0	37.0
9	37.7362727435218	39.0	38.0	39.0	35.0	39.0
10	37.53691874965879	39.0	37.0	39.0	35.0	39.0
11	37.271997152128534	39.0	37.0	39.0	34.0	39.0
12	36.38749598957177	38.0	35.0	39.0	33.0	39.0
13	36.12589192297884	38.0	35.0	39.0	33.0	39.0
14	37.08606958588449	39.0	35.0	41.0	33.0	41.0
15	37.27236435404247	39.0	35.0	41.0	33.0	41.0
16	37.3685847170713	39.0	35.0	41.0	33.0	41.0
17	37.31037162179848	39.0	35.0	41.0	33.0	41.0
18	37.26048152061977	38.0	35.0	41.0	33.0	41.0
19	37.20229310490532	38.0	35.0	41.0	33.0	41.0
20	37.04345098004022	38.0	35.0	41.0	33.0	41.0
21	36.901542023678914	38.0	35.0	41.0	32.0	41.0
22	36.891365071245396	38.0	35.0	41.0	32.0	41.0
23	36.89786028228927	38.0	35.0	41.0	33.0	41.0
24	36.8391902412539	38.0	35.0	41.0	33.0	41.0
25	36.74131186062182	38.0	35.0	41.0	32.0	41.0
26	36.63345022469616	38.0	35.0	41.0	32.0	41.0
27	36.54834613304974	38.0	35.0	41.0	32.0	41.0
28	36.51735608639042	38.0	35.0	41.0	32.0	41.0
29	36.50886332684934	38.0	35.0	41.0	32.0	41.0
30	36.431615561284204	38.0	35.0	40.0	31.0	41.0
31	36.2927175110441	38.0	35.0	40.0	31.0	41.0
32	36.091288190681745	38.0	35.0	40.0	31.0	41.0
33	35.965429173780734	38.0	35.0	40.0	30.0	41.0
34	35.82577204015439	37.0	35.0	40.0	30.0	41.0
35	35.65346908586168	37.0	35.0	40.0	30.0	41.0
36	35.49877462253363	37.0	35.0	40.0	29.0	41.0
37	35.41729693023687	37.0	35.0	40.0	29.0	41.0
38	35.368512174127	37.0	35.0	40.0	29.0	41.0
39	35.320639813811425	37.0	35.0	40.0	28.0	41.0
40	35.15854073510682	37.0	34.0	40.0	27.0	41.0
41	35.10971110707264	36.0	34.0	40.0	27.0	41.0
42	35.08213730958412	36.0	34.0	40.0	27.0	41.0
43	34.99117743181524	36.0	34.0	40.0	27.0	41.0
44	34.86412033453515	36.0	34.0	40.0	27.0	41.0
45	34.74236485511229	36.0	34.0	40.0	26.0	41.0
46	34.71875698786738	36.0	34.0	40.0	26.0	41.0
47	34.68190517216235	35.0	34.0	40.0	26.0	41.0
48	34.61611096527736	35.0	34.0	40.0	26.0	41.0
49	34.56919288121878	36.0	34.0	40.0	26.0	41.0
50	34.40510522840184	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	8.0
12	8.0
13	15.0
14	13.0
15	46.0
16	104.0
17	237.0
18	557.0
19	919.0
20	1668.0
21	2660.0
22	4100.0
23	5839.0
24	8352.0
25	12237.0
26	17191.0
27	21552.0
28	23405.0
29	25207.0
30	28371.0
31	34059.0
32	41898.0
33	57258.0
34	117033.0
35	239547.0
36	73974.0
37	97043.0
38	164736.0
39	359097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.81578130620676	23.782269457401213	29.09473136300714	13.307217873384891
2	32.381973751420006	25.556056625377018	28.78459157948426	13.277378043718716
3	29.75980806782242	24.429920898276094	31.490518188460587	14.3197528454409
4	27.706992317178692	26.661252121133256	30.30163655386613	15.330119007821924
5	23.223314853579172	32.237560941682204	29.817842423263404	14.721281781475223
6	21.44900417981975	40.803611292468474	27.062033191762414	10.685351335949367
7	85.3945625697852	5.3480602988918875	7.2068049768946985	2.050572154428223
8	86.93479137172724	3.9755777073288567	6.833545353175698	2.256085567768198
9	82.38477824668938	6.033179796565653	8.486477471676467	3.0955644850684934
10	52.73759870888517	23.536670458344272	13.265561770317072	10.460169062453492
11	46.829162861901416	19.950132334783444	20.55657639183361	12.66412841148153
12	41.66440437381603	21.518705235581344	24.04634073196579	12.770549658636835
13	23.108816660048056	38.18578322822085	23.226979394064493	15.4784207176666
14	15.240674305363916	41.55932928439003	28.36571216605005	14.834284244196002
15	15.310151001503957	22.951241419179308	47.495585724446	14.243021854870735
16	17.29625715800676	17.868673339121813	45.67610398021447	19.158965522656956
17	17.256246358830307	19.038933125127606	29.595502038307163	34.109318477734924
18	22.07175170270256	23.378571711691905	33.24688009249599	21.30279649310954
19	29.150447335692103	24.043349270345118	26.24274664040163	20.563456753561148
20	31.193914768771236	22.319145578731906	25.41313954644955	21.073800106047315
21	23.298699686420036	26.45042886341659	28.103809701160465	22.14706174900291
22	21.95396290138871	25.242102728287787	26.62572851438781	26.178205855935694
23	20.97343656867386	28.443938887430555	26.69954282987782	23.883081714017766
24	20.578638421286044	23.20873147817841	37.921786740196794	18.290843360338755
25	19.084702487923842	24.142217076908235	35.184823716906024	21.5882567182619
26	17.070850524889334	32.67401519213784	28.488287305956973	21.766846977015852
27	18.49725421216493	32.004900014134655	29.644337649264585	19.85350812443583
28	16.003272659013014	28.439077762296964	36.718845235985185	18.838804342704833
29	17.575510100296228	24.69855415181219	36.64076808768572	21.085167660205858
30	19.00176421449079	28.959592084293405	31.719888508225395	20.318755192990405
31	28.634644565748214	26.536807317713418	24.879612366403194	19.948935750135178
32	29.7753636682499	26.330546038968272	26.77657296661005	17.117517326171775
33	27.5371520836652	26.309830167245142	26.833709883564833	19.319307865524827
34	20.189748410599048	26.91552635889014	29.96120822143397	22.933517009076844
35	21.230627481510897	25.117209205624846	31.737837277949417	21.914326034914843
36	28.503618546762905	26.30601605367879	26.562907820353754	18.627457579204556
37	21.158234110290703	33.035982048238814	27.657782773518687	18.1480010679518
38	21.60044692436613	33.43078019562663	23.51423449618925	21.454538383817987
39	21.54458137860013	31.757954857348413	26.841263324157026	19.85620043989443
40	23.917408736114947	27.09179823488807	25.0459376325124	23.944855396484584
41	17.97853476714089	26.118301836981793	26.76198959120929	29.141173804668025
42	22.61544985225919	26.64173283405839	23.992569209334256	26.750248104348163
43	22.606625040478214	26.656465782540185	25.84458309869056	24.89232607829104
44	20.68027332984828	29.9403427766298	28.213297196476955	21.166086697044957
45	18.869840757019276	35.08700292191014	23.345815206945574	22.69734111412501
46	21.10506087998331	33.74241570995985	26.07036366451057	19.082159745546274
47	22.74670023086605	27.144148813249778	27.218037915280313	22.891113040603855
48	22.81714915203281	23.756243741301393	31.566052594382484	21.860554512283315
49	20.404460568422582	25.359218450736986	31.980893534628784	22.25542744621165
50	19.75187321587359	31.83423712867548	27.7941934234212	20.61969623202973
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	661.0
1	1681.5
2	2702.0
3	7511.0
4	12320.0
5	9048.5
6	5777.0
7	5772.0
8	5767.0
9	5728.0
10	5689.0
11	5596.0
12	5503.0
13	5093.0
14	4683.0
15	4527.0
16	4371.0
17	3985.0
18	3599.0
19	3456.5
20	3314.0
21	3547.0
22	3780.0
23	5067.5
24	6355.0
25	6969.5
26	7584.0
27	9764.5
28	11945.0
29	15596.5
30	19248.0
31	20784.5
32	22321.0
33	26808.5
34	31296.0
35	30479.5
36	29663.0
37	33951.0
38	38239.0
39	48016.0
40	57793.0
41	81302.0
42	104811.0
43	120287.5
44	135764.0
45	141665.5
46	147567.0
47	133919.0
48	120271.0
49	112234.0
50	104197.0
51	100873.0
52	97549.0
53	88334.5
54	79120.0
55	72529.0
56	65938.0
57	61353.5
58	56769.0
59	54204.0
60	51639.0
61	44106.0
62	36573.0
63	29167.5
64	21762.0
65	17324.0
66	12886.0
67	10359.5
68	7833.0
69	6275.0
70	4717.0
71	3916.0
72	3115.0
73	2516.5
74	1918.0
75	1470.5
76	1023.0
77	772.0
78	521.0
79	383.0
80	245.0
81	186.0
82	127.0
83	105.5
84	84.0
85	58.5
86	33.0
87	28.5
88	24.0
89	22.0
90	20.0
91	12.0
92	4.0
93	4.5
94	5.0
95	5.0
96	5.0
97	7.0
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1337139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.265008061438614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23557656633925	12.247967230993439
2	6.583306142184123	2.0098844266271794
3	2.1202437044962656	0.970966117240499
4	1.1026919471154317	0.6733040584800202
5	0.7392257965147441	0.5642143871510473
6	0.5206847407129099	0.4768954058670387
7	0.4055930489097831	0.43339668128899134
8	0.35957621842191134	0.4391147098329671
9	0.3088673879610771	0.42433768504271974
>10	4.319382941292172	15.997860042590952
>50	1.3928720675989326	15.36443712225923
>100	1.9025681406993276	48.610296518964894
>500	0.007429971911088235	0.7620216403450185
>1k	0.0014859943822176469	0.5783591513196116
>5k	4.953314607392157E-4	0.4469448219963687
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5911	0.4420632409943917	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4566	0.3414753439993898	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2062	0.15420984654549752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013536363833528152	0.0
2	0.0	0.0	0.0	0.04719030706605671	0.0
3	0.0	0.0	0.0	0.074263034733113	0.0
4	0.0	0.0	0.0	0.12825891698619216	0.0
5	0.0	0.0	0.0	0.24073787392335427	0.0
6	0.0	0.0	0.0	0.3606954849121894	0.0
7	0.0	0.0	0.0	0.4473730853710796	0.0
8	0.0	0.0	0.0	0.7176516427985422	0.0
9	0.0	0.0	0.0	0.8518186964855561	0.0
10	7.478654051673013E-5	0.0	0.0	1.0603235714461996	0.0
11	7.478654051673013E-5	0.0	0.0	1.1872363307030906	0.0
12	7.478654051673013E-5	0.0	0.0	1.3396513002761867	0.0
13	7.478654051673013E-5	0.0	0.0	1.3906557209085966	0.0
14	7.478654051673013E-5	0.0	0.0	1.4041920847421248	0.0
15	7.478654051673013E-5	0.0	0.0	1.4302925873824637	0.0
16	7.478654051673013E-5	0.0	0.0	1.485634627364844	0.0
17	7.478654051673013E-5	0.0	0.0	1.5492031868040645	0.0
18	7.478654051673013E-5	0.0	0.0	1.6656458303886132	0.0
19	7.478654051673013E-5	0.0	0.0	1.7144066548055212	0.0
20	7.478654051673013E-5	0.0	0.0	1.7669068062482658	0.0
21	7.478654051673013E-5	0.0	0.0	1.8266612521211332	0.0
22	7.478654051673013E-5	0.0	0.0	1.8953900828560082	0.0
23	7.478654051673013E-5	0.0	0.0	1.965839004022768	0.0
24	7.478654051673013E-5	0.0	0.0	2.022078482491349	0.0
25	7.478654051673013E-5	0.0	0.0	2.06620254139622	0.0
26	7.478654051673013E-5	0.0	0.0	2.1111492522467747	0.0
27	7.478654051673013E-5	0.0	0.0	2.1557220303947457	0.0
28	1.4957308103346026E-4	0.0	0.0	2.206128158703022	0.0
29	1.4957308103346026E-4	0.0	0.0	2.260647546739718	0.0
30	1.4957308103346026E-4	0.0	0.0	2.334985368013348	0.0
31	1.4957308103346026E-4	0.0	0.0	2.4054342891801075	0.0
32	1.4957308103346026E-4	0.0	0.0	2.471769950618447	0.0
33	1.4957308103346026E-4	0.0	0.0	2.535936802381802	0.0
34	1.4957308103346026E-4	0.0	0.0	2.5992062156589553	0.0
35	1.4957308103346026E-4	0.0	0.0	2.685435096874745	0.0
36	1.4957308103346026E-4	0.0	0.0	2.760670356634576	0.0
37	1.4957308103346026E-4	0.0	0.0	2.834783818286655	0.0
38	1.4957308103346026E-4	0.0	0.0	2.9123374608025046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAA	25	4.4432323E-5	44.0	37
CGTATGT	30	2.5280297E-6	44.0	22
CGAAACG	20	7.856945E-4	44.0	32
CGACGGT	145	0.0	44.0	28
CGACGAA	25	4.4432323E-5	44.0	10
CGCATCC	25	4.4432323E-5	44.0	29
GAATCGT	20	7.856945E-4	44.0	12
GCACGAC	25	4.4432323E-5	44.0	13
ATCACGC	20	7.856945E-4	44.0	29
ATCGTCA	25	4.4432323E-5	44.0	20
CGTTACC	20	7.856945E-4	44.0	38
AACGCGT	20	7.856945E-4	44.0	19
CGAATTA	40	8.314601E-9	44.0	16
ATGACGG	60	0.0	44.0	2
CGTACGA	20	7.856945E-4	44.0	22
TCCGTAC	20	7.856945E-4	44.0	32
CGTAAGA	20	7.856945E-4	44.0	25
TAGGCGA	20	7.856945E-4	44.0	1
GACGAAT	20	7.856945E-4	44.0	25
TTACCCG	25	4.4432323E-5	44.0	29
>>END_MODULE