FastQCFastQC Report
Thu 26 May 2016
SRR1545820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545820_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6301960
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502360.7971488235406128No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA96040.15239703203447816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT305950.041.7708781
CGGTCTA8650.041.20231231
CTATACG1750.040.228571
ACTATCG557.8216544E-1140.016
TACGGGT6650.038.7067644
AATGCGG7400.037.4594572
AGGGCGA40300.037.449136
GGGCGAT71050.037.280797
CGTTAGG9800.037.0408172
ACGGGCC6100.036.7868845
ACGGGTG10000.036.525
CGGGATT13750.036.486
CGTAATC1450.036.4137933
GCGCGAC9500.036.3578959
TAGGACG3150.036.3174631
CGACGGT9700.036.2886628
CGTCGAA553.430614E-936.030
CGTTAGT800.035.752
TAGGGAC41850.035.746715
GGCGATA14600.035.7123268
Produced by FastQC (version 0.11.5)