##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545820_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	6301960
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.663466604040647	31.0	31.0	33.0	30.0	34.0
2	32.080000031736155	33.0	31.0	34.0	30.0	34.0
3	32.1202435432786	33.0	31.0	34.0	30.0	34.0
4	35.84090029133793	37.0	35.0	37.0	35.0	37.0
5	35.83889218592311	37.0	35.0	37.0	35.0	37.0
6	35.892098172632004	37.0	35.0	37.0	35.0	37.0
7	36.08100590927267	37.0	35.0	37.0	35.0	37.0
8	36.02569629131254	37.0	35.0	37.0	35.0	37.0
9	37.78166062621788	39.0	38.0	39.0	35.0	39.0
10	37.48900484928498	39.0	37.0	39.0	35.0	39.0
11	37.3155661095913	39.0	37.0	39.0	34.0	39.0
12	36.84529939891716	39.0	35.0	39.0	33.0	39.0
13	36.67879247091381	39.0	35.0	39.0	33.0	39.0
14	37.785347891767	40.0	36.0	41.0	33.0	41.0
15	37.905811366622444	40.0	36.0	41.0	33.0	41.0
16	37.93010983884379	40.0	35.0	41.0	33.0	41.0
17	37.8258424363214	40.0	35.0	41.0	33.0	41.0
18	37.7155881662213	39.0	36.0	41.0	33.0	41.0
19	37.641831112860125	39.0	36.0	41.0	33.0	41.0
20	37.50079848174219	39.0	35.0	41.0	33.0	41.0
21	37.386021809087964	39.0	35.0	41.0	33.0	41.0
22	37.41081980844055	39.0	35.0	41.0	33.0	41.0
23	37.387784276637746	39.0	35.0	41.0	33.0	41.0
24	37.35098556639522	39.0	35.0	41.0	33.0	41.0
25	37.26275714222242	39.0	35.0	41.0	33.0	41.0
26	37.162605602066655	39.0	35.0	41.0	33.0	41.0
27	37.05984344553123	39.0	35.0	41.0	32.0	41.0
28	36.99587176053164	39.0	35.0	41.0	32.0	41.0
29	36.91496756564624	39.0	35.0	41.0	32.0	41.0
30	36.76851122507918	39.0	35.0	41.0	31.0	41.0
31	36.629875784676514	39.0	35.0	41.0	31.0	41.0
32	36.44407120959194	39.0	35.0	41.0	31.0	41.0
33	36.25194304629036	39.0	35.0	41.0	30.0	41.0
34	36.01870862398365	39.0	35.0	41.0	29.0	41.0
35	35.79283714907743	38.0	35.0	41.0	28.0	41.0
36	35.64529971627874	38.0	35.0	41.0	27.0	41.0
37	35.590120692609915	38.0	35.0	41.0	27.0	41.0
38	35.49159832813918	38.0	35.0	41.0	27.0	41.0
39	35.42801604580163	38.0	35.0	41.0	26.0	41.0
40	35.2844522973805	38.0	35.0	41.0	25.0	41.0
41	35.14671832255362	38.0	34.0	40.0	25.0	41.0
42	35.10990913937886	38.0	34.0	40.0	24.0	41.0
43	35.034437857428486	38.0	34.0	40.0	24.0	41.0
44	34.89958727126164	38.0	34.0	40.0	24.0	41.0
45	34.77588797770852	38.0	34.0	40.0	23.0	41.0
46	34.72659934369625	37.0	34.0	40.0	23.0	41.0
47	34.65245669601203	37.0	34.0	40.0	23.0	41.0
48	34.56943554068893	37.0	34.0	40.0	23.0	41.0
49	34.489887273165806	37.0	34.0	40.0	24.0	41.0
50	34.366318097861615	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	7.0
11	28.0
12	36.0
13	61.0
14	100.0
15	207.0
16	441.0
17	1013.0
18	2275.0
19	4484.0
20	8105.0
21	12835.0
22	19567.0
23	28558.0
24	42419.0
25	64348.0
26	94631.0
27	117030.0
28	122175.0
29	124454.0
30	133783.0
31	154233.0
32	186463.0
33	241866.0
34	434802.0
35	721353.0
36	391063.0
37	528174.0
38	912642.0
39	1954802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.09750934629861	21.911690965985187	27.385511809024493	15.605287878691708
2	33.70819554551282	23.675761191756216	29.183174758329155	13.432868504401805
3	29.11151451294518	23.933331852312616	33.13408844232588	13.821065192416327
4	26.07804556042882	26.62201918133406	32.40715586896775	14.89277938926937
5	22.975534595586137	30.882804714723672	31.36078299449695	14.780877695193242
6	19.89371560593847	40.4195520123898	29.277034446426192	10.409697935245543
7	84.41583253464002	4.3655307237748255	9.283556226951616	1.9350805146335426
8	85.4914344108817	3.2373896375096	9.233651118064856	2.03752483354385
9	80.8737916457737	5.01437647969838	10.873982062723343	3.2378498118045815
10	41.02553808656354	26.972925883375964	19.126367035017676	12.875168995042813
11	35.35579407041619	24.867739560390735	25.011647170086764	14.764819199106311
12	32.6816419018845	22.927232162692242	28.538867273038864	15.8522586623844
13	22.840846339868868	30.536563227948132	29.220353667747812	17.40223676443519
14	18.463049590920917	32.17713219379368	31.446248468730364	17.913569746555037
15	18.54864200978743	24.346536633047496	39.8314651314829	17.273356225682168
16	20.67147363677332	21.888380757732513	38.25238497229434	19.18776063319983
17	20.573329567309216	22.05563031183949	31.560720791626736	25.81031932922456
18	23.03018426013494	23.634313769049626	33.53950199620436	19.79599997461107
19	25.252112041333174	25.525947483005286	29.36997378593327	19.851966689728275
20	27.126481285187467	24.28779300408127	29.357961650026343	19.227764060704924
21	23.70898894946969	26.050197081542887	31.386346469987114	18.85446749900031
22	23.393356987349968	23.25444782258218	31.038629251851805	22.313565938216048
23	21.175602510964843	26.562450412252698	30.232530831677764	22.029416245104695
24	20.78559686192867	24.583034484509582	35.6223936680017	19.008974985560048
25	20.205221867482496	25.912700175818316	33.29341982494335	20.588658131755835
26	19.814105452906713	27.97532831055735	30.570362236510544	21.64020400002539
27	19.484604789621006	28.11176205497972	31.866641489314436	20.536991666084838
28	18.178598404305962	27.183812655110472	34.80974173114396	19.827847209439604
29	19.508914686859328	25.251858152066976	34.706361195564554	20.532865965509142
30	21.05302159962932	27.080432119531068	32.87891386171921	18.987632419120402
31	25.831487346793697	25.430532723152798	29.349503963846168	19.388475966207338
32	25.84678417508204	25.351541425207397	29.785733327409254	19.015941072301317
33	24.605519552647113	26.00048238960577	29.492681641901886	19.90131641584523
34	20.96701661070524	26.46340186227777	31.421383188722242	21.14819833829475
35	20.91412830294067	26.811547518549784	31.172825597115818	21.101498581393724
36	25.127293730839295	27.5872903033342	28.148607734736498	19.13680823109001
37	21.252118388564828	29.590270328596187	29.354153311033393	19.803457971805596
38	21.159512278719635	30.373915416790965	27.786990079276926	20.679582225212474
39	21.57838196370653	28.774904950205965	28.725539356009875	20.921173730077626
40	23.367476150277056	26.484823769113103	28.70692609918184	21.440773981428
41	19.860503716304134	26.445312252061264	29.725244209737923	23.96893982189668
42	21.90021834476893	26.427301982240447	27.650651543329374	24.021828129661248
43	21.619638969463466	26.533887869805582	28.305320884296314	23.541152276434634
44	20.810652558886442	28.11895029482891	29.51169160070835	21.5587055455763
45	20.45922221023301	30.443481075728823	27.397603285327104	21.69969342871107
46	21.741267796050753	29.072494906346595	28.59719833194752	20.589038965655128
47	21.22398428425442	27.70025515871253	29.74096947616297	21.334791080870076
48	21.71886206830891	26.358656671892554	31.008543373807512	20.913937885991025
49	21.496899377336575	26.22434925007458	31.32333432773296	20.95541704485589
50	20.944737827596494	28.711115272074082	29.546363353623317	20.797783546706103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3507.0
1	9354.5
2	15202.0
3	58198.5
4	101195.0
5	72028.0
6	42861.0
7	40493.5
8	38126.0
9	37789.5
10	37453.0
11	36121.0
12	34789.0
13	33164.0
14	31539.0
15	29981.0
16	28423.0
17	26716.5
18	25010.0
19	25748.0
20	26486.0
21	28921.5
22	31357.0
23	32391.0
24	33425.0
25	41895.5
26	50366.0
27	57116.0
28	63866.0
29	74816.0
30	85766.0
31	104196.5
32	122627.0
33	140993.0
34	159359.0
35	186948.0
36	214537.0
37	224126.0
38	233715.0
39	269025.0
40	304335.0
41	363329.5
42	422324.0
43	466076.5
44	509829.0
45	521918.5
46	534008.0
47	519940.0
48	505872.0
49	507831.0
50	509790.0
51	478677.0
52	447564.0
53	423385.0
54	399206.0
55	366724.5
56	334243.0
57	304979.5
58	275716.0
59	255521.0
60	235326.0
61	202049.0
62	168772.0
63	137471.0
64	106170.0
65	87975.0
66	69780.0
67	54562.0
68	39344.0
69	31566.0
70	23788.0
71	19486.5
72	15185.0
73	12451.5
74	9718.0
75	7446.0
76	5174.0
77	4179.5
78	3185.0
79	2368.5
80	1552.0
81	1123.0
82	694.0
83	545.0
84	396.0
85	287.5
86	179.0
87	136.0
88	93.0
89	71.5
90	50.0
91	38.0
92	26.0
93	22.5
94	19.0
95	14.0
96	9.0
97	6.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	6301960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.24790766312384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56202205750459	11.478402514298715
2	7.790526859214002	2.2199741467433456
3	2.5503901693444155	1.0901317091327403
4	1.2539188088609916	0.7146287762282256
5	0.7829148985900276	0.5577449591597339
6	0.49989966620519544	0.42735145709508326
7	0.38561933447288765	0.384598806947948
8	0.3127021174683528	0.35642807166019164
9	0.2525436616812345	0.32383968952872744
>10	2.8323306056106703	9.615294807044927
>50	1.0018296253786962	10.253651880228734
>100	1.7063321697564482	55.42659494904114
>500	0.06601579086526045	5.5596779901649365
>1k	0.0027269861975320877	0.6229243107018902
>5k	1.1362442489717033E-4	0.15548014657676992
>10k+	1.1362442489717033E-4	0.8132757854467529
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50236	0.7971488235406128	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	9604	0.15239703203447816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0225009362166691	0.0
2	0.0	0.0	0.0	0.07515122279417832	0.0
3	0.0	0.0	0.0	0.1087598144069464	0.0
4	0.0	0.0	0.0	0.15538023091228761	0.0
5	0.0	0.0	0.0	0.27984944366514547	0.0
6	0.0	0.0	0.0	0.40525487308710306	0.0
7	1.5868079137284275E-5	0.0	0.0	0.49805139988194147	0.0
8	1.5868079137284275E-5	0.0	0.0	0.7127782467676723	0.0
9	3.173615827456855E-5	0.0	0.0	0.8132866600232309	0.0
10	3.173615827456855E-5	0.0	0.0	0.9938495325263886	0.0
11	3.173615827456855E-5	0.0	0.0	1.1472462535465158	0.0
12	3.173615827456855E-5	0.0	0.0	1.2795066931557801	0.0
13	3.173615827456855E-5	0.0	0.0	1.330966873797993	0.0
14	3.173615827456855E-5	0.0	0.0	1.3498657560504985	0.0
15	4.760423741185282E-5	0.0	0.0	1.3816971227998909	0.0
16	4.760423741185282E-5	0.0	0.0	1.4507867393636265	0.0
17	6.34723165491371E-5	0.0	0.0	1.5355857542732738	0.0
18	6.34723165491371E-5	0.0	0.0	1.6557071133425156	0.0
19	6.34723165491371E-5	0.0	0.0	1.7092936165891246	0.0
20	6.34723165491371E-5	0.0	0.0	1.7691797472532356	0.0
21	6.34723165491371E-5	0.0	0.0	1.848139309040362	0.0
22	6.34723165491371E-5	0.0	0.0	1.9328431154751855	0.0
23	6.34723165491371E-5	0.0	0.0	2.0277977010326946	0.0
24	6.34723165491371E-5	0.0	0.0	2.105630629201074	0.0
25	7.934039568642137E-5	0.0	0.0	2.171610102253902	0.0
26	7.934039568642137E-5	0.0	0.0	2.2385099238966926	0.0
27	7.934039568642137E-5	0.0	0.0	2.3098052034605105	0.0
28	7.934039568642137E-5	0.0	0.0	2.380814857599858	0.0
29	7.934039568642137E-5	0.0	0.0	2.460409142552476	0.0
30	7.934039568642137E-5	0.0	0.0	2.5549035538150036	0.0
31	7.934039568642137E-5	0.0	0.0	2.6403214238110047	0.0
32	7.934039568642137E-5	0.0	0.0	2.7237399158357083	0.0
33	7.934039568642137E-5	0.0	0.0	2.8152352601412893	0.0
34	7.934039568642137E-5	0.0	0.0	2.9068892852382433	0.0
35	9.520847482370564E-5	0.0	0.0	3.0119677052853397	0.0
36	9.520847482370564E-5	0.0	0.0	3.1017334289649567	0.0
37	9.520847482370564E-5	0.0	0.0	3.1975131546376048	0.0
38	9.520847482370564E-5	0.0	0.0	3.3088753340230657	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	30595	0.0	41.770878	1
CGGTCTA	865	0.0	41.202312	31
CTATACG	175	0.0	40.22857	1
ACTATCG	55	7.8216544E-11	40.0	16
TACGGGT	665	0.0	38.706764	4
AATGCGG	740	0.0	37.459457	2
AGGGCGA	4030	0.0	37.44913	6
GGGCGAT	7105	0.0	37.28079	7
CGTTAGG	980	0.0	37.040817	2
ACGGGCC	610	0.0	36.786884	5
ACGGGTG	1000	0.0	36.52	5
CGGGATT	1375	0.0	36.48	6
CGTAATC	145	0.0	36.41379	33
GCGCGAC	950	0.0	36.357895	9
TAGGACG	315	0.0	36.317463	1
CGACGGT	970	0.0	36.28866	28
CGTCGAA	55	3.430614E-9	36.0	30
CGTTAGT	80	0.0	35.75	2
TAGGGAC	4185	0.0	35.74671	5
GGCGATA	1460	0.0	35.712326	8
>>END_MODULE