FastQCFastQC Report
Thu 26 May 2016
SRR1545819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545819_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2768858
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150370.543075881825648No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA135660.48994928595110326No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA43340.15652662577857007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG1450.044.0000041
TCACGGT454.802132E-1044.03
GCCCCGT454.802132E-1044.042
TATTTCG700.044.01
AACGTAC700.044.026
CGAACAC302.5290155E-644.015
GATCGCG1050.044.01
AGTCACG600.044.019
CTCCGAT254.444472E-544.017
GGTCGAC551.8189894E-1244.09
CGACGTA254.444472E-544.022
ACGCATG750.044.01
ACGTTAC207.858406E-444.023
TCCGAAC207.858406E-444.027
TTCGTCG408.3200575E-944.017
TGTATCG750.044.03
ACTTACG950.044.01
CCGCTCG502.7284841E-1144.019
CCTCGGT750.044.040
TCGATAC254.444472E-544.023
Produced by FastQC (version 0.11.5)