##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545819_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2768858
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.690491170005828	31.0	31.0	33.0	30.0	34.0
2	32.10449867779424	33.0	31.0	34.0	30.0	34.0
3	32.14660520691202	33.0	31.0	34.0	30.0	34.0
4	35.83300227025005	37.0	35.0	37.0	35.0	37.0
5	35.817857037088935	37.0	35.0	37.0	35.0	37.0
6	35.862757497856514	37.0	35.0	37.0	35.0	37.0
7	36.09696849748163	37.0	35.0	37.0	35.0	37.0
8	36.060307173571196	37.0	35.0	37.0	35.0	37.0
9	37.87802985924161	39.0	38.0	39.0	35.0	39.0
10	37.55132910391215	39.0	37.0	39.0	35.0	39.0
11	37.27500940821089	39.0	37.0	39.0	34.0	39.0
12	36.23156622694266	37.0	35.0	39.0	33.0	39.0
13	35.88917127566672	37.0	35.0	39.0	32.0	39.0
14	36.79176360795678	38.0	35.0	41.0	32.0	41.0
15	37.05188637337126	38.0	35.0	41.0	33.0	41.0
16	37.202469754678646	38.0	35.0	41.0	33.0	41.0
17	37.15392880386065	38.0	35.0	41.0	33.0	41.0
18	37.09940488100148	38.0	35.0	41.0	33.0	41.0
19	37.012788665940974	37.0	35.0	41.0	33.0	41.0
20	36.83749473609698	37.0	35.0	41.0	33.0	41.0
21	36.69390412942809	37.0	35.0	41.0	32.0	41.0
22	36.65565153575951	37.0	35.0	41.0	32.0	41.0
23	36.690922033560405	37.0	35.0	41.0	33.0	41.0
24	36.63112842912132	37.0	35.0	41.0	33.0	41.0
25	36.54871828024406	37.0	35.0	41.0	32.0	41.0
26	36.44111110067761	37.0	35.0	40.0	32.0	41.0
27	36.35038272096294	37.0	35.0	40.0	32.0	41.0
28	36.35690345983795	36.0	35.0	40.0	32.0	41.0
29	36.364277980308124	36.0	35.0	40.0	32.0	41.0
30	36.284675487150295	36.0	35.0	40.0	32.0	41.0
31	36.09694502209936	36.0	35.0	40.0	31.0	41.0
32	35.8833403518707	36.0	35.0	40.0	31.0	41.0
33	35.70302160674184	36.0	35.0	40.0	30.0	41.0
34	35.532008864304345	36.0	35.0	40.0	30.0	41.0
35	35.326819215719986	36.0	35.0	40.0	29.0	41.0
36	35.15004886491109	36.0	35.0	40.0	28.0	41.0
37	35.060134900381314	36.0	34.0	40.0	27.0	41.0
38	35.01594556311664	36.0	34.0	40.0	27.0	41.0
39	35.03179794702365	36.0	34.0	40.0	27.0	41.0
40	34.802054132064555	36.0	34.0	40.0	26.0	41.0
41	34.79708060146096	36.0	34.0	40.0	26.0	41.0
42	34.789377786798745	36.0	34.0	40.0	27.0	41.0
43	34.69546903452615	35.0	34.0	40.0	26.0	41.0
44	34.553943178017796	35.0	34.0	40.0	26.0	41.0
45	34.46457239771776	35.0	34.0	40.0	25.0	41.0
46	34.43304026425335	35.0	34.0	40.0	25.0	41.0
47	34.375676903618746	35.0	34.0	40.0	25.0	41.0
48	34.33416809384952	35.0	34.0	40.0	24.0	41.0
49	34.31970509141314	36.0	34.0	40.0	24.0	41.0
50	34.10115939495633	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	5.0
12	7.0
13	12.0
14	22.0
15	42.0
16	143.0
17	353.0
18	845.0
19	1729.0
20	3370.0
21	5443.0
22	8567.0
23	12607.0
24	19174.0
25	28811.0
26	41231.0
27	49316.0
28	52271.0
29	53516.0
30	59652.0
31	71076.0
32	90522.0
33	121564.0
34	250455.0
35	565935.0
36	146301.0
37	178525.0
38	298911.0
39	708449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.18933582003844	23.880639599430523	29.386953032622117	14.54307154790892
2	31.020659058716625	25.346875860011604	30.735812381855627	12.89665269941615
3	27.621748749845604	24.77165676246308	33.63845310954914	13.96814137814218
4	25.03573675500874	26.860351812913486	31.90654775362261	16.19736367845516
5	22.16892307225578	32.38197841853934	31.840816683267974	13.608281825936903
6	20.038766885120147	39.58101137725373	29.348995145290946	11.031226592335178
7	85.86453332023527	4.962479115938773	7.637228055754394	1.5357595080715587
8	87.33777607952449	3.6084912985786923	7.423385381265489	1.6303472406313362
9	84.01590114047018	4.658310393671326	8.950115896156467	2.3756725697020213
10	55.10813483392793	19.915683650082453	15.41025216894474	9.565929347044882
11	49.53424841577286	19.4662564855258	20.09261580044914	10.906879298252203
12	44.42611358184493	20.77307684251052	23.696989878137483	11.103819697507058
13	19.965090300766597	45.273972157474304	22.900199287937482	11.860738253821612
14	13.314225576031708	46.07336309771032	27.4585406691134	13.15387065714457
15	12.300125177961457	22.92284400283438	52.00660344445255	12.77042737475161
16	12.64550222510508	17.666019709208634	51.08037320801573	18.60810485767056
17	13.461867672520583	18.36280517094051	30.767666669796718	37.40766048674219
18	20.320760400136084	23.596262430214914	36.14717692276022	19.93580024688879
19	27.27976660413788	24.997309360032187	27.500001805798636	20.222922230031298
20	30.79236999513879	21.77208076398284	27.313751734469587	20.12179750640878
21	20.403104817942992	28.833584098570604	29.732330079765738	21.030981003720665
22	20.809445627041907	25.404697532340048	26.454588859378127	27.33126798123992
23	16.640037156112736	31.08100884913564	26.36498513105403	25.913968863697594
24	18.007243419489193	23.65206160807091	43.3809895632062	14.959705409233697
25	13.932205985283463	25.954202057310273	38.44772826919979	21.665863688206475
26	14.024554527534455	35.54447356997	30.3823453568222	20.048626545673343
27	15.66743401070044	36.58002685583732	30.32416252476653	17.428376608695714
28	12.280767016582288	29.74901565916345	41.3139640963892	16.65625322786506
29	13.081169204054524	23.901478515691306	39.89482306423804	23.122529216016133
30	15.987674340829324	32.07622059347211	34.43087366704974	17.505231398648828
31	28.009923224665183	27.805542935029532	26.058649450423243	18.12588438988204
32	28.31546435389608	27.60293232805727	27.881855985391812	16.199747332654834
33	27.423074783900077	28.35605870723598	26.243274302979785	17.977592205884157
34	18.3440248651249	28.920804172695025	28.969741315733778	23.765429646446297
35	18.377793299620276	26.610032005975025	32.016882050289325	22.995292644115374
36	29.783073021440607	25.50827814210768	27.583176890978155	17.12547194547355
37	18.101975615939857	33.674171806571515	31.32992013313792	16.893932444350703
38	18.954565383995856	33.32352182741044	26.507859919143563	21.214052869450146
39	18.958032517377198	32.48646192762504	29.33527107565646	19.220234479341304
40	22.827172791092934	27.643996189042557	26.03102073129066	23.497810288573845
41	15.495016356924046	25.086588044601783	29.471031017119692	29.94736458135448
42	21.4286178633935	24.871589658985762	25.53395659871326	28.165835878907476
43	22.400823733105852	26.865335817149166	26.12156347490554	24.612276974839446
44	18.24351411303866	32.13321159842795	28.553649193999835	21.06962509453356
45	14.840522699250016	41.2919694690013	22.436289618319176	21.431218213429506
46	21.296577867120668	35.04181146162064	26.023544724937143	17.63806594632155
47	20.73797211702442	28.656976991958416	27.556270491300022	23.04878039971714
48	22.14299180384115	24.984668769579372	32.48606465192509	20.38627477465439
49	20.682497982922925	24.85002842327053	32.69857103542327	21.768902558383278
50	17.91789250297415	34.73605363655341	28.10747246698819	19.238581393484246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2815.0
1	4180.0
2	5545.0
3	16653.5
4	27762.0
5	20790.0
6	13818.0
7	14347.5
8	14877.0
9	15613.5
10	16350.0
11	16145.0
12	15940.0
13	15007.0
14	14074.0
15	12960.0
16	11846.0
17	11026.0
18	10206.0
19	10342.5
20	10479.0
21	10355.0
22	10231.0
23	10553.0
24	10875.0
25	11072.0
26	11269.0
27	17909.0
28	24549.0
29	31653.5
30	38758.0
31	45484.0
32	52210.0
33	61414.0
34	70618.0
35	80677.5
36	90737.0
37	98206.5
38	105676.0
39	129290.0
40	152904.0
41	205425.5
42	257947.0
43	306691.0
44	355435.0
45	350796.0
46	346157.0
47	302436.0
48	258715.0
49	244887.5
50	231060.0
51	200691.5
52	170323.0
53	153508.0
54	136693.0
55	122119.5
56	107546.0
57	87298.5
58	67051.0
59	59076.0
60	51101.0
61	42403.5
62	33706.0
63	26879.0
64	20052.0
65	14527.0
66	9002.0
67	6854.5
68	4707.0
69	4058.0
70	3409.0
71	2753.0
72	2097.0
73	1592.0
74	1087.0
75	823.5
76	560.0
77	432.0
78	304.0
79	237.5
80	171.0
81	135.0
82	99.0
83	70.0
84	41.0
85	30.5
86	20.0
87	14.5
88	9.0
89	12.5
90	16.0
91	11.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2768858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.411426330020387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.00964760214934	10.59632068177098
2	9.223658839813263	2.4740484204679376
3	3.3299303148514245	1.3397734530519438
4	1.5604367096294953	0.8371072789542156
5	0.8380037333558719	0.5619412667092162
6	0.58332794945462	0.46939558922114955
7	0.41211840661598076	0.3868966954702929
8	0.3084802656960267	0.3309728286117902
9	0.2321381454792451	0.2801973272834206
>10	2.2170015091040245	6.557226736509349
>50	0.6318730120844575	6.1038810272163015
>100	1.395621349255073	47.215873988768564
>500	0.24795303159115167	20.156149561323552
>1k	0.009264179202306766	1.6449786021900552
>5k	0.0	0.0
>10k+	5.44951717782751E-4	1.0452365424512307
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15037	0.543075881825648	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	13566	0.48994928595110326	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	4334	0.15652662577857007	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04431429853029661	0.0
2	0.0	0.0	0.0	0.1809410233388639	0.0
3	0.0	0.0	0.0	0.25949326400992756	0.0
4	0.0	0.0	0.0	0.3737642017033737	0.0
5	0.0	0.0	0.0	0.6474149270204539	0.0
6	0.0	0.0	0.0	0.8636773716817547	0.0
7	0.0	0.0	0.0	1.01438932585203	0.0
8	0.0	0.0	0.0	1.364533681395001	0.0
9	0.0	0.0	0.0	1.4911201657867612	0.0
10	3.611597272232812E-5	0.0	0.0	1.7375033316984836	0.0
11	3.611597272232812E-5	0.0	0.0	1.963517088994813	0.0
12	3.611597272232812E-5	0.0	0.0	2.165080332758126	0.0
13	3.611597272232812E-5	0.0	0.0	2.2456911838743627	0.0
14	3.611597272232812E-5	0.0	0.0	2.2729948592524427	0.0
15	3.611597272232812E-5	0.0	0.0	2.3237378009273137	0.0
16	3.611597272232812E-5	0.0	0.0	2.4501798214281845	0.0
17	3.611597272232812E-5	0.0	0.0	2.6068509110976437	0.0
18	3.611597272232812E-5	0.0	0.0	2.852114481854974	0.0
19	3.611597272232812E-5	0.0	0.0	2.940815310861012	0.0
20	3.611597272232812E-5	0.0	0.0	3.0302384593214966	0.0
21	3.611597272232812E-5	0.0	0.0	3.167876431366289	0.0
22	3.611597272232812E-5	0.0	0.0	3.2993385720755635	0.0
23	3.611597272232812E-5	0.0	0.0	3.4665555257799423	0.0
24	7.223194544465625E-5	0.0	0.0	3.583751857263897	0.0
25	7.223194544465625E-5	0.0	0.0	3.684118145459247	0.0
26	7.223194544465625E-5	0.0	0.0	3.78856553857222	0.0
27	7.223194544465625E-5	0.0	0.0	3.884959069768114	0.0
28	7.223194544465625E-5	0.0	0.0	3.989623158717421	0.0
29	7.223194544465625E-5	0.0	0.0	4.098910092175186	0.0
30	7.223194544465625E-5	0.0	0.0	4.246479956718618	0.0
31	7.223194544465625E-5	0.0	0.0	4.389607556617205	0.0
32	7.223194544465625E-5	0.0	0.0	4.511607312473229	0.0
33	7.223194544465625E-5	0.0	0.0	4.635701794747148	0.0
34	7.223194544465625E-5	0.0	0.0	4.763082830538799	0.0
35	7.223194544465625E-5	0.0	0.0	4.94272367885966	0.0
36	7.223194544465625E-5	0.0	0.0	5.083720436367629	0.0
37	7.223194544465625E-5	0.0	0.0	5.22692026821166	0.0
38	7.223194544465625E-5	0.0	0.0	5.378751817536327	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	145	0.0	44.000004	1
TCACGGT	45	4.802132E-10	44.0	3
GCCCCGT	45	4.802132E-10	44.0	42
TATTTCG	70	0.0	44.0	1
AACGTAC	70	0.0	44.0	26
CGAACAC	30	2.5290155E-6	44.0	15
GATCGCG	105	0.0	44.0	1
AGTCACG	60	0.0	44.0	19
CTCCGAT	25	4.444472E-5	44.0	17
GGTCGAC	55	1.8189894E-12	44.0	9
CGACGTA	25	4.444472E-5	44.0	22
ACGCATG	75	0.0	44.0	1
ACGTTAC	20	7.858406E-4	44.0	23
TCCGAAC	20	7.858406E-4	44.0	27
TTCGTCG	40	8.3200575E-9	44.0	17
TGTATCG	75	0.0	44.0	3
ACTTACG	95	0.0	44.0	1
CCGCTCG	50	2.7284841E-11	44.0	19
CCTCGGT	75	0.0	44.0	40
TCGATAC	25	4.444472E-5	44.0	23
>>END_MODULE