FastQCFastQC Report
Thu 26 May 2016
SRR1545818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545818_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1455943
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86920.5970013935985131No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC68940.4735075480290094No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA53210.36546760415758034No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT44790.3076356698030074No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA32860.2256956487994379No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATG25400.17445737916937681No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTC14690.10089680708654117No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA207.857174E-444.00000429
CGAGTTA207.857174E-444.00000416
CCGGCTA207.857174E-444.00000418
TGGCGTA207.857174E-444.00000427
TAGCGAA207.857174E-444.00000415
AAGCGTA207.857174E-444.00000410
TAACGCG207.857174E-444.0000041
CGCTAAT408.314601E-944.00000412
TATTCGT408.314601E-944.00000434
GTCACGA207.857174E-444.0000049
TCGCTAA408.314601E-944.00000411
AACGCAT207.857174E-444.00000418
TATTACG408.314601E-944.0000041
CGTCGTG207.857174E-444.00000415
ACTCCGT207.857174E-444.00000416
CACGTAG408.314601E-944.0000041
ATCGGCG207.857174E-444.00000434
ATCGGCC207.857174E-444.00000433
CCTATCG207.857174E-444.0000041
TTACCCG207.857174E-444.00000438