##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545818_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1455943
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.628324048400245	31.0	31.0	33.0	30.0	34.0
2	32.031439417614564	33.0	31.0	34.0	30.0	34.0
3	32.073066734068576	33.0	31.0	34.0	30.0	34.0
4	35.785285550327174	37.0	35.0	37.0	35.0	37.0
5	35.75454190170906	37.0	35.0	37.0	35.0	37.0
6	35.80513797586856	37.0	35.0	37.0	35.0	37.0
7	35.99636936336107	37.0	35.0	37.0	35.0	37.0
8	35.9264188227149	37.0	35.0	37.0	35.0	37.0
9	37.68732361088312	39.0	38.0	39.0	35.0	39.0
10	37.45560506146188	39.0	37.0	39.0	35.0	39.0
11	37.17183845796161	39.0	37.0	39.0	34.0	39.0
12	36.32319603171278	38.0	35.0	39.0	33.0	39.0
13	36.01012127535213	38.0	35.0	39.0	32.0	39.0
14	37.00530240538263	39.0	35.0	41.0	33.0	41.0
15	37.186434496405425	39.0	35.0	41.0	33.0	41.0
16	37.3141627110402	39.0	35.0	41.0	33.0	41.0
17	37.22705284478857	38.0	35.0	41.0	33.0	41.0
18	37.16010448211228	38.0	35.0	41.0	33.0	41.0
19	37.073197920522986	38.0	35.0	41.0	32.0	41.0
20	36.9153057502938	38.0	35.0	41.0	32.0	41.0
21	36.815297714264915	38.0	35.0	41.0	32.0	41.0
22	36.81443779049042	38.0	35.0	41.0	32.0	41.0
23	36.824783662547226	38.0	35.0	41.0	32.0	41.0
24	36.79913980148948	38.0	35.0	41.0	32.0	41.0
25	36.726786694259324	38.0	35.0	41.0	32.0	41.0
26	36.6038814706345	38.0	35.0	41.0	32.0	41.0
27	36.503376849231046	38.0	35.0	41.0	32.0	41.0
28	36.48694282674528	38.0	35.0	41.0	32.0	41.0
29	36.45954614981493	37.0	35.0	41.0	31.0	41.0
30	36.378770322739285	37.0	35.0	40.0	31.0	41.0
31	36.222462005724125	37.0	35.0	40.0	31.0	41.0
32	36.03508447789508	37.0	35.0	40.0	31.0	41.0
33	35.86483399418796	37.0	35.0	40.0	30.0	41.0
34	35.68294431856192	37.0	35.0	40.0	30.0	41.0
35	35.471995126182826	37.0	35.0	40.0	29.0	41.0
36	35.3149354061251	37.0	35.0	40.0	28.0	41.0
37	35.24204931099638	37.0	34.0	40.0	27.0	41.0
38	35.15246475995283	37.0	34.0	40.0	27.0	41.0
39	35.13563855178396	37.0	34.0	40.0	27.0	41.0
40	34.96109188340478	37.0	34.0	40.0	26.0	41.0
41	34.85771077576526	36.0	34.0	40.0	25.0	41.0
42	34.831518129487215	36.0	34.0	40.0	25.0	41.0
43	34.731121341975616	36.0	34.0	40.0	25.0	41.0
44	34.61750631721159	36.0	34.0	40.0	24.0	41.0
45	34.51490408621766	36.0	34.0	40.0	24.0	41.0
46	34.44266842864041	35.0	34.0	40.0	24.0	41.0
47	34.379143276900265	35.0	34.0	40.0	24.0	41.0
48	34.33252881465827	35.0	34.0	40.0	23.0	41.0
49	34.27639749633056	36.0	34.0	40.0	24.0	41.0
50	34.112895903205	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	15.0
13	11.0
14	14.0
15	32.0
16	67.0
17	197.0
18	498.0
19	1034.0
20	1824.0
21	2982.0
22	4654.0
23	6783.0
24	10190.0
25	15219.0
26	21373.0
27	26059.0
28	27903.0
29	29099.0
30	32683.0
31	38768.0
32	48827.0
33	64388.0
34	126327.0
35	259367.0
36	81376.0
37	100284.0
38	166529.0
39	389437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.37215605281251	23.274468849398637	28.49905525147619	13.854319846312665
2	31.01536255196804	26.634078394552535	29.678909133118538	12.671649920360895
3	28.535389091468556	25.62943741616258	32.712200958416645	13.12297253395222
4	25.167331413386375	27.061567657525053	33.1169558148911	14.654145114197464
5	23.561293264914905	30.483885701569363	31.68036111303808	14.274459920477655
6	20.48857681928482	40.01303622463242	29.13211574903688	10.36627120704588
7	84.27452173608445	4.589946172343285	9.172268419848855	1.9632636717234122
8	85.19378849309348	4.710417921580722	7.925859734893467	2.1699338504323316
9	80.52066598761077	5.476381973744851	9.682453227907962	4.320498810736409
10	50.63082826731541	25.406145707627292	14.143960306138359	9.819065718918942
11	44.17947680644091	19.36813460417063	23.443912296017082	13.008476293371373
12	41.817021682854346	20.488645503292368	25.050293864526292	12.644038949327
13	20.742020807133247	40.3041190486166	25.495640969461032	13.458219174789123
14	14.714037568778448	41.83632188897505	27.944363206526628	15.505277335719875
15	13.127643046465417	23.04211085186714	49.562791949959575	14.267454151707861
16	15.12840818630949	19.007337512526245	45.88057362135743	19.983680679806834
17	15.440302264580414	19.53977593903058	30.348921626739507	34.671000169649496
18	19.887729121263675	23.212790610621433	36.53989201500333	20.359588253111557
19	27.362953082641283	24.832290824572116	26.729892585080595	21.074863507706002
20	28.687180748147423	22.492020635423227	28.403653164993408	20.417145451435943
21	20.299764482538123	28.541295916117594	30.4726902083392	20.68624939300508
22	21.5005669864823	24.500547068120113	26.794112132137045	27.204773813260548
23	17.79918581977454	31.30981089232202	27.508975282686205	23.382028005217236
24	17.889848709736576	23.364650951307848	41.01197643039597	17.733523908559608
25	15.61956752427808	27.964762356768087	36.64037671804459	19.775293400909238
26	16.325982541898963	32.95966943760848	28.802157776781094	21.912190243711464
27	17.452056845632004	33.185433770415464	29.00044850656928	20.362060877383247
28	13.057722726782572	27.712348629032867	40.36421755522023	18.865711088964336
29	15.016453254007883	23.297890095972164	38.51146645163993	23.174190198380018
30	17.06632745924806	31.18391310648837	33.11001873012886	18.63974070413471
31	26.33468480565517	28.209002687605217	25.989272931701308	19.46703957503831
32	27.875541830964533	25.431764842442323	28.34410413045016	18.34858919614298
33	25.886727708433643	27.81317675211186	26.855103530838775	19.44499200861572
34	18.21678458566029	25.999644216840906	30.564864146467276	25.21870705103153
35	18.645372792753562	24.534133547810594	33.160776211706086	23.659717447729754
36	27.376758568158234	26.3201237960552	28.271505134472985	18.03161250131358
37	18.541728625365142	31.957088979444936	30.84523226527412	18.655950129915798
38	18.700732102836444	32.8366563800918	27.05222663249866	21.41038488457309
39	19.45714907795154	31.606594488932604	28.618359372585328	20.31789706053053
40	22.53062104766464	26.268473422379863	26.724672600507027	24.476232929448475
41	16.548381358336144	25.888032704577036	27.984062562888795	29.57952337419803
42	20.187534814206327	26.25686582510442	25.799705070871592	27.75589428981766
43	21.101650270649333	27.40931478773551	27.163219988694614	24.325814952920545
44	18.898610728579346	29.38294974459852	29.339541451828815	22.37889807499332
45	16.498860188894756	36.60878207457298	23.91474116775176	22.97761656878051
46	21.043406232249477	34.041854660518986	26.244227967715766	18.670511139515764
47	20.223250498130767	26.46470363194163	30.035585184310097	23.2764606856175
48	21.326109607312922	24.39051528802982	32.08181913715028	22.20155596750697
49	19.53565489857776	24.0591836356231	33.980519841779525	22.42464162401962
50	18.23320006346402	32.841876364665374	28.36793748106897	20.556986090801633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	838.0
1	1642.5
2	2447.0
3	9255.5
4	16064.0
5	11706.5
6	7349.0
7	7295.5
8	7242.0
9	7458.5
10	7675.0
11	7457.5
12	7240.0
13	6776.5
14	6313.0
15	5888.0
16	5463.0
17	5294.0
18	5125.0
19	4623.0
20	4121.0
21	4669.0
22	5217.0
23	5610.0
24	6003.0
25	7046.5
26	8090.0
27	10350.5
28	12611.0
29	15694.5
30	18778.0
31	23025.5
32	27273.0
33	29499.5
34	31726.0
35	39694.0
36	47662.0
37	49968.0
38	52274.0
39	66666.0
40	81058.0
41	108226.0
42	135394.0
43	157586.0
44	179778.0
45	172513.0
46	165248.0
47	152132.0
48	139016.0
49	127409.5
50	115803.0
51	104557.0
52	93311.0
53	84446.5
54	75582.0
55	66930.5
56	58279.0
57	50996.5
58	43714.0
59	38767.5
60	33821.0
61	28846.0
62	23871.0
63	18547.5
64	13224.0
65	10368.0
66	7512.0
67	5677.0
68	3842.0
69	3309.5
70	2777.0
71	2262.0
72	1747.0
73	1445.5
74	1144.0
75	882.5
76	621.0
77	490.5
78	360.0
79	265.0
80	170.0
81	129.0
82	88.0
83	61.5
84	35.0
85	24.5
86	14.0
87	10.0
88	6.0
89	7.5
90	9.0
91	7.0
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1455943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.520200535274704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3111046195644	13.8954645760892
2	8.042081162466348	2.8179774937473097
3	2.7051513156682585	1.4218438058631024
4	1.3416092983734675	0.9402105578996938
5	0.856363777057822	0.750183255259916
6	0.5891439534872337	0.6193152125544532
7	0.47461508474241065	0.5820746023227402
8	0.33377847487509305	0.46782926513358236
9	0.2935190392222135	0.4628261185284907
>10	3.472776229939487	13.990784518221764
>50	1.0171962475839589	12.867790809294238
>100	1.5523540540658336	47.89582383530533
>500	0.005549784667242511	0.609166876964975
>1k	0.0035677187146558997	1.2266680402411798
>5k	0.0011892395715519665	1.4520410325740203
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8692	0.5970013935985131	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC	6894	0.4735075480290094	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5321	0.36546760415758034	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT	4479	0.3076356698030074	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3286	0.2256956487994379	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATG	2540	0.17445737916937681	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTC	1469	0.10089680708654117	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05542799409042799	0.0
2	0.0	0.0	0.0	0.25090267956918644	0.0
3	0.0	0.0	0.0	0.3395737333123618	0.0
4	0.0	0.0	0.0	0.5582636133420058	0.0
5	0.0	0.0	0.0	1.259252594366675	0.0
6	0.0	0.0	0.0	1.5543190907885818	0.0
7	0.0	0.0	0.0	1.867586849210443	0.0
8	0.0	0.0	0.0	2.4282544028165938	0.0
9	0.0	0.0	0.0	2.6137699072010374	0.0
10	0.0	0.0	0.0	3.443610086383876	0.0
11	0.0	0.0	0.0	3.736203958534091	0.0
12	0.0	0.0	0.0	4.204422837981982	0.0
13	0.0	0.0	0.0	4.34680478562691	0.0
14	0.0	0.0	0.0	4.390075710381519	0.0
15	0.0	0.0	0.0	4.490903833460513	0.0
16	0.0	0.0	0.0	4.6437944342601325	0.0
17	0.0	0.0	0.0	4.8136499849238605	0.0
18	0.0	0.0	0.0	5.065995028651534	0.0
19	0.0	0.0	0.0	5.177400488892766	0.0
20	0.0	0.0	0.0	5.285509116771742	0.0
21	0.0	0.0	0.0	5.430569740711003	0.0
22	0.0	0.0	0.0	5.586688489865331	0.0
23	0.0	0.0	0.0	5.77323425436298	0.0
24	0.0	0.0	0.0	5.906687281026798	0.0
25	0.0	0.0	0.0	6.019603789434065	0.0
26	0.0	0.0	0.0	6.134786870090381	0.0
27	0.0	0.0	0.0	6.246535750369349	0.0
28	0.0	0.0	0.0	6.364878295372827	0.0
29	6.868400754699875E-5	0.0	0.0	6.491050817236664	0.0
30	6.868400754699875E-5	0.0	0.0	6.657197431492854	0.0
31	6.868400754699875E-5	0.0	0.0	6.8056235718019185	0.0
32	6.868400754699875E-5	0.0	0.0	6.940656330639317	0.0
33	6.868400754699875E-5	0.0	0.0	7.077474873672939	0.0
34	6.868400754699875E-5	0.0	0.0	7.225145489898987	0.0
35	6.868400754699875E-5	0.0	0.0	7.422818063619249	0.0
36	6.868400754699875E-5	0.0	0.0	7.575639980411321	0.0
37	6.868400754699875E-5	0.0	0.0	7.728393213195846	0.0
38	6.868400754699875E-5	0.0	0.0	7.858412039482315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	7.857174E-4	44.000004	29
CGAGTTA	20	7.857174E-4	44.000004	16
CCGGCTA	20	7.857174E-4	44.000004	18
TGGCGTA	20	7.857174E-4	44.000004	27
TAGCGAA	20	7.857174E-4	44.000004	15
AAGCGTA	20	7.857174E-4	44.000004	10
TAACGCG	20	7.857174E-4	44.000004	1
CGCTAAT	40	8.314601E-9	44.000004	12
TATTCGT	40	8.314601E-9	44.000004	34
GTCACGA	20	7.857174E-4	44.000004	9
TCGCTAA	40	8.314601E-9	44.000004	11
AACGCAT	20	7.857174E-4	44.000004	18
TATTACG	40	8.314601E-9	44.000004	1
CGTCGTG	20	7.857174E-4	44.000004	15
ACTCCGT	20	7.857174E-4	44.000004	16
CACGTAG	40	8.314601E-9	44.000004	1
ATCGGCG	20	7.857174E-4	44.000004	34
ATCGGCC	20	7.857174E-4	44.000004	33
CCTATCG	20	7.857174E-4	44.000004	1
TTACCCG	20	7.857174E-4	44.000004	38
>>END_MODULE