FastQCFastQC Report
Thu 26 May 2016
SRR1545817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545817_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2565999
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163150.6358147450564089No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA143510.5592753543551654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG302.528941E-644.00000422
TCGCCTA302.528941E-644.00000413
AAGTACG207.8582973E-444.00000443
ACAGTCG408.3200575E-944.00000432
TCCGCGA408.3200575E-944.00000419
GTCGACG302.528941E-644.0000048
CGTTCCG207.8582973E-444.00000426
CGTTAGT207.8582973E-444.00000414
CCCGTTA207.8582973E-444.00000412
CTACGTT207.8582973E-444.00000430
TGACACG207.8582973E-444.0000041
TCTAACG302.528941E-644.00000444
ACAGCGA302.528941E-644.00000425
CGTAAGT207.8582973E-444.00000435
CCGCGAA408.3200575E-944.00000420
TAGTGTC600.044.00000444
CGCGTGG408.3200575E-944.0000042
ATCGGCC302.528941E-644.00000436
TACCGTA207.8582973E-444.00000444
CTTACGA408.3200575E-944.00000433
Produced by FastQC (version 0.11.5)