##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545817_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2565999 Sequences flagged as poor quality 0 Sequence length 50 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71683543134662 31.0 31.0 33.0 30.0 34.0 2 32.12818672181868 33.0 31.0 34.0 30.0 34.0 3 32.16300785775832 33.0 31.0 34.0 30.0 34.0 4 35.842720905191314 37.0 35.0 37.0 35.0 37.0 5 35.81656773833505 37.0 35.0 37.0 35.0 37.0 6 35.870684283197306 37.0 35.0 37.0 35.0 37.0 7 36.11052342576907 37.0 35.0 37.0 35.0 37.0 8 36.08264695348673 37.0 35.0 37.0 35.0 37.0 9 37.91398827513183 39.0 39.0 39.0 35.0 39.0 10 37.63707546261709 39.0 37.0 39.0 35.0 39.0 11 37.2537518525923 39.0 37.0 39.0 34.0 39.0 12 35.99652571961252 37.0 35.0 39.0 33.0 39.0 13 35.55337589765234 37.0 35.0 39.0 32.0 39.0 14 36.364099908067 37.0 35.0 41.0 32.0 41.0 15 36.67857080224895 37.0 35.0 41.0 33.0 41.0 16 36.839906017110685 37.0 35.0 41.0 33.0 41.0 17 36.83096486007984 37.0 35.0 41.0 33.0 41.0 18 36.792568508405495 37.0 35.0 40.0 33.0 41.0 19 36.69717252422935 36.0 35.0 40.0 33.0 41.0 20 36.490245319659124 36.0 35.0 40.0 33.0 41.0 21 36.31574758992502 35.0 35.0 40.0 32.0 41.0 22 36.29981461411325 35.0 35.0 40.0 32.0 41.0 23 36.33719771519786 35.0 35.0 40.0 32.0 41.0 24 36.2822495254285 35.0 35.0 40.0 32.0 41.0 25 36.18113530052038 35.0 35.0 40.0 32.0 41.0 26 36.06340727334656 35.0 35.0 40.0 32.0 41.0 27 35.96809040065877 35.0 35.0 40.0 32.0 41.0 28 36.0091145787664 36.0 35.0 40.0 32.0 41.0 29 36.01363562495543 36.0 35.0 40.0 32.0 41.0 30 35.9689263323953 36.0 35.0 40.0 32.0 41.0 31 35.7476826764157 35.0 35.0 40.0 31.0 41.0 32 35.506039168370684 35.0 35.0 40.0 31.0 41.0 33 35.346642379829454 35.0 35.0 40.0 30.0 41.0 34 35.1725413766724 35.0 35.0 40.0 30.0 41.0 35 34.95869250143901 35.0 34.0 40.0 29.0 41.0 36 34.7498759742307 35.0 34.0 40.0 27.0 41.0 37 34.62070561991646 35.0 34.0 40.0 27.0 41.0 38 34.56700411808422 35.0 34.0 40.0 27.0 41.0 39 34.57855712336599 35.0 34.0 40.0 27.0 41.0 40 34.33887698319446 35.0 34.0 40.0 24.0 41.0 41 34.295372289700815 35.0 34.0 40.0 24.0 41.0 42 34.27018677715775 35.0 34.0 40.0 24.0 41.0 43 34.15984885418895 35.0 34.0 40.0 24.0 41.0 44 34.05464382488068 35.0 34.0 40.0 24.0 41.0 45 33.930985164062804 35.0 34.0 40.0 23.0 41.0 46 33.95711066138374 35.0 34.0 39.0 24.0 41.0 47 33.899098557715725 35.0 34.0 39.0 23.0 41.0 48 33.891523730133954 35.0 34.0 39.0 23.0 41.0 49 33.89292240565955 35.0 34.0 39.0 24.0 41.0 50 33.631767198662196 35.0 34.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 5.0 11 5.0 12 5.0 13 2.0 14 21.0 15 30.0 16 129.0 17 387.0 18 861.0 19 1860.0 20 3294.0 21 5409.0 22 8589.0 23 12633.0 24 18682.0 25 27705.0 26 39136.0 27 48806.0 28 51462.0 29 52444.0 30 58953.0 31 69571.0 32 87768.0 33 120465.0 34 263443.0 35 620044.0 36 126618.0 37 148870.0 38 244663.0 39 554137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.13485235185205 22.400983008956747 31.56384706307368 14.900317576117528 2 30.317977520645954 25.593190020728766 31.050323869962536 13.03850858866274 3 26.993697191620107 24.8672349443628 33.373512616333834 14.76555524768326 4 24.626626900478136 27.94642554420325 32.66365263587398 14.76329491944463 5 22.770741531855624 32.35500091777121 32.33991907245482 12.534338477918347 6 20.936835906794975 39.50644563774187 28.42089961843321 11.135818837029944 7 85.33892647658865 5.183634132359366 7.6900263795893915 1.7874130114625921 8 88.19816375610435 3.4272811485896915 6.94267612730948 1.43187896799648 9 85.45420321675886 4.171007081452487 8.242247950992967 2.132541750795694 10 62.414560566859144 18.36239998534684 11.812046692146023 7.410992755647995 11 56.12246926051023 15.670621851372507 18.900124279081947 9.306784609035311 12 50.01712783208411 20.083951708476892 20.116609554407464 9.78231090503153 13 19.81816828455506 49.50500760132798 21.012595873965655 9.664228240151301 14 11.731882982027662 49.877572048936884 27.116027714741897 11.274517254293553 15 10.175023450905476 21.04061614988938 57.44655395422992 11.337806444975232 16 11.521165830540074 15.117932625850594 55.43341988831641 17.92748165529293 17 11.985312542990078 15.30144789612155 30.01899065432216 42.694248906566216 18 19.98948557657271 20.468714134339102 38.1021582627273 21.439642026360882 19 29.219146227258857 22.59950218219103 27.55262180538652 20.628729785163596 20 32.350597174823534 20.75109927946192 25.968716277753813 20.929587267960745 21 20.074598626110145 27.521522806517073 30.247712489365743 22.156166078007043 22 21.64560469431204 23.996073264253027 26.165598661573913 28.19272337986102 23 16.05382543017359 31.44599822525262 25.046658241098303 27.45351810347549 24 17.60230615834223 21.611271087790758 45.212956045579126 15.573466708287883 25 13.136871838219735 24.22230094399881 41.65964211209747 20.981185105683984 26 13.152265452948345 36.80398940139883 29.344945185091653 20.69879996056117 27 14.908072840246625 37.61377147847681 30.10655888798086 17.37159679329571 28 10.825413415983405 29.29630915678455 44.273750691251244 15.6045267359808 29 11.83500071512109 23.79381285807204 42.63813041236571 21.73305601444116 30 15.09513448758164 32.04011381142393 34.23165792348321 18.633093777511213 31 30.674563785878327 26.57315922570508 25.384187601008417 17.36808938740818 32 31.110339481815853 25.84759386110439 27.33044712799966 15.711619529080098 33 28.637540388753074 27.923666377110823 25.243618567271458 18.19517466686464 34 17.58878316008697 27.275302913212357 29.744828427446773 25.391085499253897 35 18.736951962958674 25.455660738761004 33.004260718729824 22.803126579550497 36 32.37814979662891 23.186953697176033 27.180992666014287 17.253903840180765 37 18.647863853415377 32.9697322563259 31.20542915254449 17.17697473771424 38 18.80074777893522 34.53684120687498 24.395761650725508 22.26664936346429 39 19.380872712732934 33.00574162343789 27.889488655295658 19.72389700853352 40 24.028536254300956 26.00242634545064 24.772885725988203 25.196151674260197 41 15.316451799084879 24.32865328474407 27.854921221715205 32.49997369445584 42 22.022066259573755 24.219066336346977 24.088668779683857 29.67019862439541 43 22.630679123413532 25.564390321274484 25.708310876192858 26.096619679119126 44 17.937146507071905 32.29931110651251 28.11875608681063 21.64478629960495 45 14.581650265647026 42.72308757719703 20.90296995439203 21.792292202763914 46 22.22436563693127 34.57101892869015 25.755232172732722 17.449383261645853 47 20.387887914219764 28.61743126166456 27.1250300565199 23.86965076759578 48 23.321560140904186 24.562207545677143 31.473706731764118 20.642525581654553 49 20.18835549039575 23.015909203394077 33.5042609135857 23.29147439262447 50 18.097473927308624 35.9848542419541 26.851296512586327 19.06637531815094 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2464.0 1 3622.5 2 4781.0 3 15759.0 4 26737.0 5 19803.5 6 12870.0 7 13516.0 8 14162.0 9 15087.0 10 16012.0 11 15736.5 12 15461.0 13 14888.0 14 14315.0 15 12818.5 16 11322.0 17 10107.5 18 8893.0 19 8060.0 20 7227.0 21 8139.5 22 9052.0 23 9993.5 24 10935.0 25 10511.0 26 10087.0 27 12158.5 28 14230.0 29 20582.5 30 26935.0 31 31616.0 32 36297.0 33 44375.0 34 52453.0 35 56193.5 36 59934.0 37 69899.5 38 79865.0 39 103079.5 40 126294.0 41 182470.5 42 238647.0 43 283026.0 44 327405.0 45 332325.0 46 337245.0 47 292448.0 48 247651.0 49 239355.0 50 231059.0 51 210987.0 52 190915.0 53 167955.5 54 144996.0 55 118355.5 56 91715.0 57 78613.0 58 65511.0 59 56117.0 60 46723.0 61 39427.5 62 32132.0 63 27015.5 64 21899.0 65 17029.5 66 12160.0 67 9397.0 68 6634.0 69 5770.0 70 4906.0 71 3824.0 72 2742.0 73 2185.5 74 1629.0 75 1257.5 76 886.0 77 646.5 78 407.0 79 308.0 80 209.0 81 159.0 82 109.0 83 75.5 84 42.0 85 34.5 86 27.0 87 17.0 88 7.0 89 8.5 90 10.0 91 6.5 92 3.0 93 2.0 94 1.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2565999.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.331383946785817 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5213111832935 10.601291313363184 2 9.180606853988916 2.4478038967003934 3 3.2288878763666764 1.2913663200289816 4 1.4515614576814388 0.7740529245882941 5 0.8686057824423017 0.5789858592068317 6 0.5639836739859886 0.4511209738575656 7 0.40815895392430845 0.3808926608258393 8 0.29966769633228973 0.31959880930036594 9 0.2555833347550281 0.30665516094172507 >10 2.0844876483255153 6.1181970734845255 >50 0.592484979282125 5.639229477819036 >100 1.230226592774377 42.87412311178433 >500 0.30289781942785265 25.149501699621947 >1k 0.010944550116045455 1.8578960191607645 >5k 0.0 0.0 >10k+ 5.915973035700247E-4 1.2092846993162119 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16315 0.6358147450564089 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 14351 0.5592753543551654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03803586829145296 0.0 2 0.0 0.0 0.0 0.1566641296430747 0.0 3 0.0 0.0 0.0 0.21656282796680748 0.0 4 0.0 0.0 0.0 0.3181217139991091 0.0 5 0.0 0.0 0.0 0.5445052784510048 0.0 6 0.0 0.0 0.0 0.7338272540246509 0.0 7 0.0 0.0 0.0 0.8787220883562309 0.0 8 0.0 0.0 0.0 1.2402576930076745 0.0 9 0.0 0.0 0.0 1.3769685802683478 0.0 10 0.0 0.0 0.0 1.6122375729686567 0.0 11 0.0 0.0 0.0 1.826618015049889 0.0 12 0.0 0.0 0.0 2.0277872282880858 0.0 13 0.0 0.0 0.0 2.0982471154509414 0.0 14 0.0 0.0 0.0 2.1204996572485024 0.0 15 0.0 0.0 0.0 2.1690967143790783 0.0 16 0.0 0.0 0.0 2.2684732145258044 0.0 17 0.0 0.0 0.0 2.40222229237034 0.0 18 0.0 0.0 0.0 2.628138202703898 0.0 19 0.0 0.0 0.0 2.708107056939617 0.0 20 0.0 0.0 0.0 2.7890501905885388 0.0 21 0.0 0.0 0.0 2.905924748996395 0.0 22 0.0 0.0 0.0 3.0181617373974037 0.0 23 0.0 0.0 0.0 3.158574886428249 0.0 24 0.0 0.0 0.0 3.2561587124546816 0.0 25 3.897117652812803E-5 0.0 0.0 3.340102626696269 0.0 26 3.897117652812803E-5 0.0 0.0 3.4330488827158545 0.0 27 7.794235305625606E-5 0.0 0.0 3.512394198127123 0.0 28 7.794235305625606E-5 0.0 0.0 3.59968963355013 0.0 29 7.794235305625606E-5 0.0 0.0 3.6910770425085904 0.0 30 7.794235305625606E-5 0.0 0.0 3.8247481780000694 0.0 31 1.1691352958438409E-4 0.0 0.0 3.955574417604995 0.0 32 1.1691352958438409E-4 0.0 0.0 4.061342190702335 0.0 33 1.1691352958438409E-4 0.0 0.0 4.168629839684271 0.0 34 1.1691352958438409E-4 0.0 0.0 4.284880859267677 0.0 35 1.1691352958438409E-4 0.0 0.0 4.441817787146449 0.0 36 1.1691352958438409E-4 0.0 0.0 4.5669932061547955 0.0 37 1.1691352958438409E-4 0.0 0.0 4.692831135164122 0.0 38 1.1691352958438409E-4 0.0 0.0 4.812823387694228 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCCG 30 2.528941E-6 44.000004 22 TCGCCTA 30 2.528941E-6 44.000004 13 AAGTACG 20 7.8582973E-4 44.000004 43 ACAGTCG 40 8.3200575E-9 44.000004 32 TCCGCGA 40 8.3200575E-9 44.000004 19 GTCGACG 30 2.528941E-6 44.000004 8 CGTTCCG 20 7.8582973E-4 44.000004 26 CGTTAGT 20 7.8582973E-4 44.000004 14 CCCGTTA 20 7.8582973E-4 44.000004 12 CTACGTT 20 7.8582973E-4 44.000004 30 TGACACG 20 7.8582973E-4 44.000004 1 TCTAACG 30 2.528941E-6 44.000004 44 ACAGCGA 30 2.528941E-6 44.000004 25 CGTAAGT 20 7.8582973E-4 44.000004 35 CCGCGAA 40 8.3200575E-9 44.000004 20 TAGTGTC 60 0.0 44.000004 44 CGCGTGG 40 8.3200575E-9 44.000004 2 ATCGGCC 30 2.528941E-6 44.000004 36 TACCGTA 20 7.8582973E-4 44.000004 44 CTTACGA 40 8.3200575E-9 44.000004 33 >>END_MODULE